Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus

Çalı́k, Pınar; Angardi, Vahideh; Haykır, Nazife Işık; Boyaci, İsmail Hakkı

doi:10.1016/j.bej.2008.08.003

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…9, Srih-Belghith Medium [ 19 ]; Medium No. 10, Hasal Medium [ 6 ]; Medium No. 11, Dhungel Medium [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Xylose-independent GI producer or an agro-residue rich in xylose which can substitute for pure and expensive xylose can be useful in making the production technology economic. There are reports on Bacillus being used for GI production on xylan containing media also [ 6 ]. Most of the researchers have suggested the requirement of cobalt and magnesium in the production medium as cobalt provides thermostability and magnesium is required for optimum activity of the enzyme.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Fermentation Medium for the Production of Glucose Isomerase UsingStreptomyces sp. SB-P1

Bhasin

Modi

2012

Biotechnology Research International

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The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries.Streptomycesare well-known producers of numerous enzymes including glucose isomerase. An array of 75 isolates was screened for the production of glucose isomerase. The isolateStreptomyces sp. SB-P1was found to produce maximum amount of extracellular GI. Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers. Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production. Quantitative effect of carbon, nitrogen, and inducer on GI was also determined. Plackett-Burman design was used to study the effect of different medium ingredients. Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.

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“…9, Srih-Belghith Medium [ 19 ]; Medium No. 10, Hasal Medium [ 6 ]; Medium No. 11, Dhungel Medium [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Medium for the Production of Glucose Isomerase UsingStreptomyces sp. SB-P1

Bhasin

Modi

2012

Biotechnology Research International

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“…Intracellular soluble GI activity was determined by measuring the conversion of D‐glucose into D‐fructose. The same procedure as described previously was followed throughout sample preparation . The enzymatic activity was determined by cysteine–carbazole–sulfuric acid method .…”

Section: Experimental Workmentioning

confidence: 99%

Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3)

Angardi

Çalı́k

2012

J of Chemical Tech & Biotech

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BACKGROUND: The effects of pretreated beet molasses based feeding strategies on thermostable glucose isomerase (GI) production by recombinant Escherichia coli BL21 (DE3) pLysS were investigated. RESULTS: The thermostable GI encoding gene of Thermus thermophilus (xylA) was recombined with pRSETA vector, and the pRSETA::xylA obtained was transferred into E.coli BL21 (DE3) pLysS and used for GI production. The highest soluble GI activity was obtained at t = 30 h, as A = 16 400 U L−1 (20.6 U mg−1 protein) under molasses based fed‐batch operation, with a specific growth rate µ = 0.1 h−1 (M‐0.1); on the other hand, the highest cell concentration was obtained at µ = 0.15 h−1 operation as 9.6 g L−1 at t = 32 h. The highest oxygen uptake was 4.57 mol m−3 s−1 at M‐0.1 operation. CONCLUSIONS: Molasses based fed‐batch operations were more successful in terms of cell concentration and thermostable enzyme production due to the existence of a natural sugar inducer, galactose, in the molasses composition. This study demonstrates the significance of proper feeding strategy development for over‐production of enzymes by recombinant E. coli strains. © 2012 Society of Chemical Industry

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Application of Microbial Enzymes in the Food Industry

Vermelho

Cardoso

Nascimento

et al. 2015

Advances in Food Biotechnology

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Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus

Cited by 8 publications

References 22 publications

Optimization of Fermentation Medium for the Production of Glucose Isomerase UsingStreptomyces sp. SB-P1

Optimization of Fermentation Medium for the Production of Glucose Isomerase UsingStreptomyces sp. SB-P1

Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3)

Application of Microbial Enzymes in the Food Industry

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