Glucose-induced expression of the homeotic transcription factor Prep1 is associated with histone post-translational modifications in skeletal muscle

Ciccarelli, Marco; Vastolo, Viviana; Albano, Luigi; Lecce, Manuela; Cabaro, Serena; Liotti, Antonietta; Longo, Michele; Oriente, Francesco; Russo, Gian Luigi; Macchia, Paolo Emidio; Formisano, Pietro; Béguinot, Francesco; Ungaro, Paola

doi:10.1007/s00125-015-3774-6

Cited by 28 publications

(26 citation statements)

References 54 publications

(57 reference statements)

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“…This is intriguing because human endothelial cells stimulated by hyperglycemia alters the subcellular locality and this is associated with distinguishable histone and non-histone regulatory events. While these (33,51) and other studies indicate biochemical properties as a glycemic sensor (52), nuclear accumulation with specific nucleosomal preferences highlights dynamic Set7 activity. This fascinating link with extracellular signaling also carries with it precise instructions for Set7 activity that include broad substrate specificity (30).…”

Section: Discussionmentioning

confidence: 81%

Set7 mediated interactions regulate transcriptional networks in embryonic stem cells

Tuano¹,

Okabe²,

Ziemann³

et al. 2016

Nucleic Acids Res

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Histone methylation by lysine methyltransferase enzymes regulate the expression of genes implicated in lineage specificity and cellular differentiation. While it is known that Set7 catalyzes mono-methylation of histone and non-histone proteins, the functional importance of this enzyme in stem cell differentiation remains poorly understood. We show Set7 expression is increased during mouse embryonic stem cell (mESC) differentiation and is regulated by the pluripotency factors, Oct4 and Sox2. Transcriptional network analyses reveal smooth muscle (SM) associated genes are subject to Set7-mediated regulation. Furthermore, pharmacological inhibition of Set7 activity confirms this regulation. We observe Set7-mediated modification of serum response factor (SRF) and mono-methylation of histone H4 lysine 4 (H3K4me1) regulate gene expression. We conclude the broad substrate specificity of Set7 serves to control key transcriptional networks in embryonic stem cells.

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Section: Discussionmentioning

confidence: 81%

Set7 mediated interactions regulate transcriptional networks in embryonic stem cells

Tuano¹,

Okabe²,

Ziemann³

et al. 2016

Nucleic Acids Res

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“…Knockdown of SET7 in a hyperglycemia model reduced the induction of oxidant and inflammatory pathways. In a related publication on Prep1 , a gene exerting major effects on the insulin sensitivity of glucose transport, it was shown that high glucose (HG) exposure induces NF-κB recruitment and histone modification at the Prep1 5′ region 168 . HG exposure also resulted in the recruitment of EP300 and SET7 (which was confirmed by an increase in H3K9/14 acetylation and H3K4me/me2) at the Prep1 promoter.…”

Section: Metabolic Diseasesmentioning

confidence: 94%

“…SET7 is reported to have multiple roles in diabetes and related diseases 168-172,180 . Perhaps one of the underlying mechanisms is due to SET7 methylation of HIF-1α at K32 and HIF-2α at K29 180 .…”

Section: Metabolic Diseasesmentioning

confidence: 99%

Chemical probes targeting epigenetic proteins: Applications beyond oncology

Ackloo

Brown

Müller³

2017

Epigenetics

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Epigenetic chemical probes are potent, cell-active, small molecule inhibitors or antagonists of specific domains in a protein; they have been indispensable for studying bromodomains and protein methyltransferases. The Structural Genomics Consortium (SGC), comprising scientists from academic and pharmaceutical laboratories, has generated most of the current epigenetic chemical probes. Moreover, the SGC has shared about 4 thousand aliquots of these probes, which have been used primarily for phenotypic profiling or to validate targets in cell lines or primary patient samples cultured in vitro. Epigenetic chemical probes have been critical tools in oncology research and have uncovered mechanistic insights into well-established targets, as well as identify new therapeutic starting points. Indeed, the literature primarily links epigenetic proteins to oncology, but applications in inflammation, viral, metabolic and neurodegenerative diseases are now being reported. We summarize the literature of these emerging applications and provide examples where existing probes might be used.

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“…Accumulation of lipids that occurs in AT during obesity triggers an inflammatory response that results in an increased secretion of several inflammatory cytokines (Haase et al, 2014;Raciti et al, 2017). Such molecules can also activate JNK and NF-κB signaling pathways in the liver and skeletal muscle, thus inhibiting systemic insulin signaling (Hotamisligil et al, 1993;Ciccarelli et al, 2016).…”

Section: Molecular Pathways Linking Obesity-induced Inflammation and Irmentioning

confidence: 99%

Chronic Adipose Tissue Inflammation Linking Obesity to Insulin Resistance and Type 2 Diabetes

et al. 2020

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Glucose-induced expression of the homeotic transcription factor Prep1 is associated with histone post-translational modifications in skeletal muscle

Cited by 28 publications

References 54 publications

Set7 mediated interactions regulate transcriptional networks in embryonic stem cells

Set7 mediated interactions regulate transcriptional networks in embryonic stem cells

Chemical probes targeting epigenetic proteins: Applications beyond oncology

Chronic Adipose Tissue Inflammation Linking Obesity to Insulin Resistance and Type 2 Diabetes

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