Glucose‐induced and fructose‐induced deboronation reaction of 4‐mercaptophenylboronic acid assembled on silver investigated by surface‐enhanced Raman scattering

Abstract

We examined the deboronation reaction of 4-mercaptophenylboronic acid (4MPBA) via fructose and glucose on silver surfaces by means of surface-enhanced Raman scattering (SERS) at the excitation wavelengths of 488, 514, and 633 nm. The SERS spectra on silver nanoparticles clearly exhibited specific spectral signatures of thiophenol (TP) peaks, indicating a deboronation reaction of 4MPBA on the surfaces, whereas no strong TP peaks were observed on gold nanoparticles. The vibrational bands at 417, 999, 1021, and 1574 cm À1 in the Ag SERS spectra could correspond to the in-plane aromatic ring modes in TP. X-ray photoelectron spectroscopy also supported the surface reaction on Ag by referring the B1s peaks at~193 eV. The ratiometric Raman measurements of the band at 1574 cm À1 , with respect to that at 1587 cm À1 , revealed fructose and glucose quantification in the concentration range of 1-10 mM. We did not identify such changes for mannose, sucrose, and sialic acid. The SERS peaks of 4MPBA on roughened Ag plates also exhibited TP bands to show the time-dependent spectral change. Our findings indicate that the deboronation of 4MPBA and conjugation with fructose and glucose may be facilitated efficiently on silver surfaces for their quantification.

An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

“…[35][36][37] In the latter case, the intensity ratios of 1574 cm À1 and 1586 cm À1 peaks (I 1574 /I 1586 ) were used to monitor glycoprotein recognition. It should be noted that a laser wavelength of 780 nm and a power of 30 mW were employed in the whole measurements to avoid the possible deboronation reaction of 4-MPBA when binding with glucose/ glycoprotein on silver surfaces, 38 and the exposure time for spectra collection was limited to 9 s. Fig. 2B shows the extinction spectra of the silver nanoparticle film before and after AFP binding to the 4-MPBA/AgNF substrate.…”

Vinyl‐functionalized polyborosiloxane for improving mechanical and flame‐retardancy performances of silicone rubber foam composites

“…Figure 2(d) presents the XPS survey spectrum of pBS. As shown, pBS possesses obvious C1s (287.5 eV), O1s (535.2 eV), B1s (193.4 eV), Si2s (156.2 eV) and Si2p (105.5 eV) peaks, [26][27][28] and the C, O, Si and B element contents are 52.6%, 33.5%, 11.2% and 2.7%, respectively (Table S1). In addition, SEM-EDS mapping was carried out to analyze the element content and distribution of pBS (Fig.…”

Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering

“…The pair of intense bands at 1586/1573 cm −1 is ascribed to the original and OH - -associated forms of C-C stretching. The SERS spectra at 824 and 417 cm −1 were assigned as the C-C out-plane bending and the C-S stretching with weak intensities [ 18 , 34 , 45 , 46 ].…”

“…When SiO 2 @Au@Ag@4-MPBA was incubated with H 2 O 2 , the new bands could be observed clearly at 390 cm −1 , 1170 cm −1 , and 1597 cm −1 . According to previous report, boronated molecules reacts selectively with H 2 O 2 to convert to its corresponding phenol form, so 4-MPBA on the surface of SiO 2 @Au@Ag can converted to 4-MPheOH or thiophenol [ 34 , 46 , 47 ]. Thus, we observed the SERS band of both 4-MPheOH and thiophenol in ethanol to compare with 4-MPBA in the presence of H 2 O 2 ( Figure S2b ).…”