Glucose effect on degradation kinetics of methyl parathion by filamentous fungi species Aspergilus Niger AN400

Abstract

This study evaluated the glucose effect on the removal of methyl parathion by Aspergillus niger AN400. The study was conducted in two stages: toxicity tests on plates and assays in flasks, under an agitation of 200 rpm. The methyl parathion concentrations in the toxicity test ranged from 0.075 to 60 mg/L. The second stage consisted on evaluating reactors: six control reactors with methyl parathion solution; six reactors with fungi and methyl parathion, and six reactors containing fungi, methyl parathion, and glucose. The reaction times studied ranged from 1 to 27 days. Methyl parathion concentrations of up to 60 mg/L were not toxic for Aspergillus niger AN400. The first-order kinetic model served as a good representation of the methyl parathion conversion rate. The first-order kinetic constant was 0.063 ± 0.005 h -l for flasks without addition of glucose, while a value of 0.162 ± 0.014 h -l was obtained when glucose was added.Keywords: Aspergillus niger; biological reactors; glucose; methyl parathion; toxicity. ResumoEste estudo avaliou o efeito da glicose na remoção de metil paration por Aspergillus niger AN400. O estudo foi realizado em duas etapas: testes de toxicidade em placas e ensaios em batelada, sob agitação de 200 rpm. As concentrações de metil paration no ensaio de toxicidade variaram de 0,075 a 60 mg/L. A segunda etapa consistiu na avaliação dos reatores divididos em lotes: seis reatores controle, com solução de metil paration; seis reatores com fungos e paration metílico e seis reatores com fungos, paration metílico e glicose. Os tempos de reação estudados variaram de 1 a 27 dias. Concentrações de paration metílico de até 60 mg/L não foram tóxicas para Aspergillus niger AN400. O modelo de primeira ordem representou bem a cinética de degradação do metil paration. A constante cinética foi de 0,063 ± 0,005 h l para reatores sem adição de glicose, enquanto o valor de 0,162 ± 0,014 h l foi obtido quando a glicose foi adicionada.

Biodegradation of Paracetamol by Native Fungal Species Inhabiting Wastewater of a Pharmaceutical Factory in Sana’a, Yemen

“…The optimum pH for paracetamol degradation was 6.0 for both fungal species. This result is consistent with that by Marinho et al, 28 who reported that the optimum pH for atrazine degradation by A. niger was 5. Also, Govarthanan et al, 26 recorded that decane degradation by Penicillium sp.…”

Optimization of aqueous methylparathion biodegradation by Fusarium sp in batch scale process using response surface methodology

“…Biodegradation of organophosphorus pesticides by organophosphorus hydrolase has been reported by Richins et al (1997). Marinho et al (2011) reported that the presence of glucose in the medium helped the removal of the pollutant. Fungus possesses several enzymatic systems such as glucose oxidase, catalase, lactanase, cytochrome P450 monooxygenase and ligninolytic enzymes.…”

“…Its system plays a central role in the oxidative metabolism as well as in the detoxification of xenobiotics. The enzyme catalyses the epoxidation of the aromatic ring, producing arene oxides that are formed through the epoxide hydrolase trans-dihydrodiols or rearranged nonenzymatically to form phenols (Marinho et al 2011) Therefore, this enzymatic system must have been activated, promoting the partial degradation of methylparathion. The enzymatic action of the fungus may have been responsible for the degradation of methylparathion.…”

Review on Performance of Aspergillus and Penicillium Species in Biodegradation of Organochlorine and Organophosphorus Pesticides

“…Marinho et al [ 52 ] also hypothesized that the addition of glucose should improve the efficiency of A. niger to biodegrade methyl parathion in batch reactors. Since the presence of glucose increased the conversion rate, it proved indispensable for the efficient removal of methyl parathion by submerged culture of fungus.…”