Glucose-driven TOR–FIE–PRC2 signalling controls plant development

Ye, Ruiqiang; Wang, Meiyue; Du, Hao; Chhajed, Shweta; Koh, Jin; Liu, Kun-Hsiang; Shin, Jinwoo; Wu, Yue; Shi, Lin; Xu, Lin; Chen, Sixue; Zhang, Yijing; Sheen, Jen

doi:10.1038/s41586-022-05171-5

Cited by 52 publications

(18 citation statements)

References 86 publications

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“…GhMSI1-A/D and GhFIE-A/D showed strong fluorescent signals in the nucleus and detectable fluorescent signals in the cytoplasm, in line with their Arabidopsis homologs [ 53 ]. However, it remains unclear whether GhFIE-A/D dynamically translocate from the cytoplasm to the nucleus driven by the direct phosphorylation by TOR kinase as Arabidopsis FIE [ 54 ]. Unfortunately, we have not successfully cloned the full-length CDS of GhVRN2-A/D.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification and characterization of polycomb repressive complex 2 core components in upland cotton (Gossypium hirsutum L.)

et al. 2023

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Background The evolutionarily conserved Polycomb Repressive Complex 2 (PRC2) plays a vital role in epigenetic gene repression by depositing tri-methylation on lysine residue K27 of histone H3 (H3K27me3) at the target loci, thus participating in diverse biological processes. However, few reports about PRC2 are available in plant species with large and complicated genomes, like cotton. Results Here, we performed a genome-wide identification and comprehensive analysis of cotton PRC2 core components, especially in upland cotton (Gossypium hirsutum). Firstly, a total of 8 and 16 PRC2 core components were identified in diploid and tetraploid cotton species, respectively. These components were classified into four groups, E(z), Su(z)12, ESC and p55, and the members in the same group displayed good collinearity, similar gene structure and domain organization. Next, we cloned G. hirsutum PRC2 (GhPRC2) core components, and found that most of GhPRC2 proteins were localized in the nucleus, and interacted with each other to form multi-subunit complexes. Moreover, we analyzed the expression profile of GhPRC2 genes. The transcriptome data and quantitative real-time PCR (qRT-PCR) assays indicated that GhPRC2 genes were ubiquitously but differentially expressed in various tissues, with high expression levels in reproductive organs like petals, stamens and pistils. And the expressions of several GhPRC2 genes, especially E(z) group genes, were responsive to various abiotic and biotic stresses, including drought, salinity, extreme temperature, and Verticillium dahliae (Vd) infection. Conclusion We identified PRC2 core components in upland cotton, and systematically investigated their classifications, phylogenetic and synteny relationships, gene structures, domain organizations, subcellular localizations, protein interactions, tissue-specific and stresses-responsive expression patterns. Our results will provide insights into the evolution and composition of cotton PRC2, and lay the foundation for further investigation of their biological functions and regulatory mechanisms.

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Section: Resultsmentioning

confidence: 99%

Genome-wide identification and characterization of polycomb repressive complex 2 core components in upland cotton (Gossypium hirsutum L.)

et al. 2023

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“…In general, the question remains how the epigenetic pathways corporate with each other and which upstream signals modulate their spatiotemporal specificity. Most recently, it has been shown that glucose-activated TOR kinase controls genome-wide H3K27me3, which limits SAM size ( Ye et al., 2022 ) indicating that glucose signaling controls stem-cell fate also epigenetically.…”

Section: Discussionmentioning

confidence: 99%

The plant stem-cell niche and pluripotency: 15 years of an epigenetic perspective

Müller-Xing

Xing

2022

Front. Plant Sci.

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Pluripotent stem-cells are slowly dividing cells giving rise to daughter cells that can either differentiate to new tissues and organs, or remain stem-cells. In plants, stem-cells are located in specific niches of the shoot and root apical meristems (SAMs and RAMs). After ablation of stem-cell niches, pluripotent meristematic cells can establish new stem-cells, whereas the removal of the whole meristem destructs the regeneration process. In tissue cultures, after detached plant organs are transferred to rooting or callus induction medium (G5 or CIM), vasculature-associated pluripotent cells (VPCs) immediately start proliferation to form adventitious roots or callus, respectively, while other cell types of the organ explants basically play no part in the process. Hence, in contrast to the widely-held assumption that all plant cells have the ability to reproduce a complete organism, only few cell types are pluripotent in practice, raising the question how pluripotent stem-cells differ from differentiated cells. It is now clear that, in addition to gene regulatory networks of pluripotency factors and phytohormone signaling, epigenetics play a crucial role in initiation, maintenance and determination of plant stem-cells. Although, more and more epigenetic regulators have been shown to control plant stem-cell fate, only a few studies demonstrate how they are recruited and how they change the chromatin structure and transcriptional regulation of pluripotency factors. Here, we highlight recent breakthroughs but also revisited classical studies of epigenetic regulation and chromatin dynamics of plant stem-cells and their pluripotent precursor-cells, and point out open questions and future directions.

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“…In turn, JMJ705 targets and regulates the transcription of energy homeostasis‐associated transcription factor genes to maintain the energy homeostasis of rice plants under starvation conditions (Wang et al., 2021). Conversely, a more recent study has revealed that an energy‐sufficient state promotes H3K27me3 through the target of rapamycin (TOR) signaling that phosphorylates and targets the fertilization‐independent endosperm (FIE) protein (a component of polycomb repressive complex 2 (PRC2), in which the E(Z) homolog functions as the catalytic subunit) to the nucleus to enhance PRC2 activity to methylate H3K27me3 (Ye et al., 2022). Subsequently, the elevated H3K27me3 and gene silencing induced by glucose‐TOR‐PRC2 signaling modulate the vernalization‐induced floral transition (Ye et al., 2022).…”

Section: Metabolic Regulation Of Dna and Histone Methylationmentioning

confidence: 99%

“…Conversely, a more recent study has revealed that an energy‐sufficient state promotes H3K27me3 through the target of rapamycin (TOR) signaling that phosphorylates and targets the fertilization‐independent endosperm (FIE) protein (a component of polycomb repressive complex 2 (PRC2), in which the E(Z) homolog functions as the catalytic subunit) to the nucleus to enhance PRC2 activity to methylate H3K27me3 (Ye et al., 2022). Subsequently, the elevated H3K27me3 and gene silencing induced by glucose‐TOR‐PRC2 signaling modulate the vernalization‐induced floral transition (Ye et al., 2022). Therefore, direct targeting of chromatin modifiers by cellular nutrient/energy signaling provides an additional layer of metabolic control of the epigenome in plants.…”

Section: Metabolic Regulation Of Dna and Histone Methylationmentioning

confidence: 99%

Metabolic regulation of the plant epigenome

Chuan

et al. 2023

The Plant Journal

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SUMMARY Chromatin modifications shape the epigenome and are essential for gene expression reprogramming during plant development and adaptation to the changing environment. Chromatin modification enzymes require primary metabolic intermediates such as S‐adenosyl‐methionine, acetyl‐CoA, alpha‐ketoglutarate, and NAD+ as substrates or cofactors. The availability of the metabolites depends on cellular nutrients, energy and reduction/oxidation (redox) states, and affects the activity of chromatin regulators and the epigenomic landscape. The changes in the plant epigenome and the activity of epigenetic regulators in turn control cellular metabolism through transcriptional and post‐translational regulation of metabolic enzymes. The interplay between metabolism and the epigenome constitutes a basis for metabolic control of plant growth and response to environmental changes. This review summarizes recent advances regarding the metabolic control of plant chromatin regulators and epigenomes, which are involved in plant adaption to environmental stresses.

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Glucose-driven TOR–FIE–PRC2 signalling controls plant development

Cited by 52 publications

References 86 publications

Genome-wide identification and characterization of polycomb repressive complex 2 core components in upland cotton (Gossypium hirsutum L.)

Genome-wide identification and characterization of polycomb repressive complex 2 core components in upland cotton (Gossypium hirsutum L.)

The plant stem-cell niche and pluripotency: 15 years of an epigenetic perspective

Metabolic regulation of the plant epigenome

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