Glucose determination in fish plasma by two different moderate methods

Abstract

Teleost fish include many different species, having a large economic and environmental impact. Glucose is a key source of energy for most vertebrate organisms. Fish as well as mammals maintain a specific level of blood sugar -glycaemia, which is influenced by multiple factors such as hormone production, diet, and temperature. Therefore, it is a useful indicator for scientists who need to check the immediate state of an organism. The most frequently used method of measuring glycaemia in farmed fish is spectrophotometry; however, this method is often inconvenient for field experiments. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. The possibility of use outside the laboratory and its small sample volume demands make this method very useful. Field studies often require fast sample processing and conditions of measurement are limited. Using the glucometer is a simple and elegant option. We tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using the human glucometer. Our results show a significant linear correlation (P < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.

Effects of unionized ammonia and suspended solids on rainbow trout (Oncorhynchus mykiss) in recirculating aquaculture systems

“…shown that devices for measuring human glucose level are also suitable for use with fish blood (Bartoňková et al, 2017;Eames et al, 2010 Bacterial species were then determined by using bacteriological standard methods and confirmed by MALDI-TOF MS (Lay, 2001).…”

The effect of temperature on the physiological condition and growth performance of freshwater eel elver Anguilla bicolor bicolor McClelland, 1844

“…SR= survival rate; SGR= specific growth rate; CV: a weight coefficient of variance; FCR= feed conversion ratio. and the blood glucose concentration can express the health status of fish (Bartoňková et al, 2016). During the elver rearing, the blood glucose was 38.54-65.45 mg/dL.…”

Improved performance of botia fish Chromobotia macracanthus with the utilization of blood clam shell in the recirculation system

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