Glucose detection based on the photothermal effect of OxTMB using a thermometer

NIR II Light-Response Au Nanoframes: Amplification of a Pressure- and Temperature-Sensing Strategy for Portable Detection and Photothermal Therapy of Cancer Cells

“…Recently, studies have found that the oxidation product of 3,3′,5,5′-tetramethylbenzidine (TMB), oxTMB, has excellent photothermal performance. 57,58 Generally, Au nanostructures have excellent peroxidase-like activity and could catalyze the oxidation of TMB to oxTMB. 69,70 To further confirm that the Au NFs exhibit peroxidase-like activity, 5 μg/mL Au NFs was added to the reaction system of TMB and H 2 O 2 .…”

Integration and Quantitative Visualization of 3,3′,5,5′-Tetramethylbenzidine-Probed Enzyme-Linked Immunosorbent Assay-like Signals in a Photothermal Bar-Chart Microfluidic Chip for Multiplexed Immunosensing

“…In comparison with the volumetric bar-chart chips, the photothermal bar-chart chips offer (1) simpler and lower cost chip manufacturing (e.g., without complicated microfluidic patterning on glass matrixes using cleanroom facilities); (2) easier controllability toward the bar-chart pumping performance by remotely manipulating the irradiation parameters; and (3) higher compatibility with a variety of commercial ELISA kits using TMB-based chromogenic substrates (e.g., no need to prepare additional specific materials, such as catalase- or platinum NP-conjugated antibodies). Given that TMB is one of the most popular chromogenic substrates in various bioanalytical settings, ,− a wide range of these conventional bioassays can be flexibly introduced in the platform to implement diverse lab-on-a-chip biosensing tasks.…”

“…Upon laser irradiation at required wavelengths, the photothermal agents can absorb the light energy and convert it into heat. The exploitation of photothermal agents as the specific biosensing probes offers an alternative way to quantitatively output the assay signals by means of portable temperature readers, such as thermometers. − A number of photothermal immunoassays have been developed based on implementing the photothermal tests in traditional vessels, such as microplates, polymerase chain reaction (PCR) tubes, and cuvettes. ,− Significantly, we for the first time discovered the near-infrared (NIR) laser-driven photothermal effect of the oxidation product of TMB and had developed a photothermal immunoassay using oxidized TMB as the photothermal probe in a conventional ELISA-like system . Although simple quantitative readout of the assay signals using digital thermometers is enabled in these photothermal immunoassays, their applicability for POC detection is still challenged in aspects of insufficient integrability and deliverability of the photothermal testing settings, due to the complex tube-by-tube or well-by-well operations of laser irradiation and temperature measurement in these traditional vessels .…”

Sweetsop-like α-Fe2O3@CoNi catalyst with superior peroxidase-like activity for sensitive and selective detection of hydroquinone

“…S4 †) were observed and they were from the oxidation of TMB to oxTMB. 44,45 The changes in solution from colorless to blue suggests the formation of oxTMB. In contrast, negligible changes of absorption spectra were observed aer reacting of TMB with commercial Fe 2 O 3 , a-Fe 2 O 3 @Co and a-Fe 2 O 3 @Ni.…”