Glucose Dehydrogenase of Bacterium anitratum: an Enzyme with a Novel Prosthetic Group

Hauge, Jens G.

doi:10.1016/s0021-9258(18)91183-x

Cited by 143 publications

(36 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PQQ is a small molecule aromatic tricyclic o-quinone compound that is ubiquitous in organisms. It is a new redox coenzyme and was first discovered as a dehydrogenase cofactor in 1964 (62). Although PQQ is widespread, there is still no evidence to prove that it can be synthesized by organisms themselves, and external intake seems to be the only source of mammals at present.…”

Section: Discussionmentioning

confidence: 99%

“…PQQ has a corresponding effect on an inflammatory response, apoptosis, and autophagy SCI model of rats. Some studies have shown that astrocytes will also undergo apoptosis after SCI (33,62). In order to observe whether PQQ affects these processes in primary cultured astrocytes, the purity of primary cultured astrocytes was identified.…”

Section: Effect Of Pqq On Primary Astrocytes Treated With Lpsmentioning

confidence: 99%

See 1 more Smart Citation

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Zhou¹,

Jin²,

Shi³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: Pyrroloquinoline quinone (PQQ) is a redox cofactor that can participate in a variety of physiological and biochemical processes, such as anti-inflammatory, cytoprotection, anti-aging, and antiapoptosis. PQQ plays an important protective role in the central nervous system (CNS). However, the effects of PQQ on astrocytes of the CNS and spinal cord injury (SCI) of rats is still unclear. The present study investigates the role of PQQ in inflammation, apoptosis, and autophagy after SCI in rats. And the effect of PQQ on lipopolysaccharide (LPS)-induced apoptosis and inflammation of astrocytes in vitro, to explore the neuroprotective mechanism of PQQ.Methods: 60 specific pathogen free (SPF) SD male rats (200-250 g) were randomly divided into Normal group, Sham group, SCI group, and SCI + PQQ group, with 15 rats in each group. BBB score, HE staining, Nissl staining, Western blot, immunofluorescence, and other methods were used for detection.Results: Our results showed that PQQ could upregulate BBB score in SCI rats. In the second place, PQQ can increase the number and improve the morphology of neurons after SCI. The expression of IL-1β, TNF-α, IL-6 was significantly decreased after PQQ treatment. And then, the ratio of B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) increased significantly, and the positive signal of NeuN increased obviously after PQQ treatment. There are a large number of co-localizations between Bcl-2 and NeuN.Meanwhile, PQQ could down-regulate the expression of Active-Caspase3, and PQQ treatment could reverse the transfer of Active-Caspase3/Caspase3 from the cytoplasm to the nucleus in neurons and astrocytes after SCI. At the same time, PQQ had no significant effect on the LC3b/a ratio. PQQ could decrease the LAMP2 expression in spinal cord after injury. The expression level of phospho-Akt (p-AKT) increased after SCI and decreased after PQQ treatment. In primary astrocytes, LPS could induce the expression levels of IL-1β, TNF-α, and IL-6, and which were inhibited by PQQ treatment at 12 hours. After treatment with LPS, the expression level of Active-Caspase3 increased, which could be reversed by PQQ treatment for 24 h.Conclusions: These results suggest that PQQ can ameliorate the motor function of hind limbs and the pathological changes of neurons and injured spinal cord after SCI, down-regulate the expressions of IL-1β, TNF-α, and IL-6, inhibit apoptosis after SCI, and inhibit LPS-induced apoptosis and inflammation of astrocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Pqq On Primary Astrocytes Treated With Lpsmentioning

confidence: 99%

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Zhou¹,

Jin²,

Shi³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…GDH was originatally investigated in Acinetobacter calcoaceticus in the early 1960s by Hauge [32][33][34][35] and subsequently in the late 1970s by Duine et al [36], who showed the enzyme to be a quinoprotein. A. calcoaceticus contains a soluble form of GDH (s-GDH) in addition to a membrane-bound form (m-GDH).…”

Section: Membrane-bound D-glucose Dehydrogenase (M-gdh)mentioning

confidence: 99%

Biooxidation with PQQ‐ and FAD‐Dependent Dehydrogenases

Adachi

Ano

Toyama

et al. 2007

Modern Biooxidation

View full text Add to dashboard Cite

Among the obligate aerobic bacteria, acetic acid bacteria are well known for their powerful ability to oxidize alcohols, sugars, or sugar alcohols and to accumulate the corresponding oxidation products in the culture medium. These reactions are restricted to one-step incomplete oxidation (so-called oxidative fermentation) and are catalyzed by primary dehydrogenases located on the outer surface of the cytoplasmic membrane, the active sites of which face the periplasmic space. All enzyme activities are linked, without exception, to the terminal ubiquinol oxidase via ubiquinone in the respiratory chain of the organisms. The respective primary dehydrogenases working in the periplasmic sugar and alcohol respirations include many unique pyrroloquinoline quinone (PQQ)-dependent dehydrogenases (quinoproteins and quinoprotein-cytochrome c complexes) and fl avin adenine dinucleotide (FAD)-dependent dehydrogenases (fl avoprotein-cytochrome c complexes). Since this sugar and alcohol respiration does not seem to generate much energy, acetic acid bacteria use rapid oxidation to produce a large number of oxidation products, compensating for the necessary bioenergy required.We have learnt a lot about the biological activities of acetic acid bacteria in the past hundred years. Among them are classic but typically important microbial bioconversions for practical use, such as the production of vinegar, D-gluconate, and L-sorbose. However, our understanding of the molecular mechanisms remains to be clarifi ed. We have been trying to uncover the enzymatic and biochemical mechanisms of the respective enzymes in acetic acid bacteria since the 1970s. In this chapter, the properties and characteristics of the individual enzymes involved in oxidative fermentation are exemplifi ed.

show abstract

“…Pyrroloquinoline quinone (PQQ) was first reported as a cofactor for bacterial dehydrogenases in the late 1960s [1]. PQQ's recognition as a cofactor was important because, at the time, only nicotinamide cofactors and flavins were considered cofactors for bacterial dehydrogenases.…”

Section: Introductionmentioning

confidence: 99%

Pyrroloquinoline-Quinone Is More Than an Antioxidant: A Vitamin-like Accessory Factor Important in Health and Disease Prevention

2021

View full text Add to dashboard Cite

Pyrroloquinoline quinone (PQQ) is associated with biological processes such as mitochondriogenesis, reproduction, growth, and aging. In addition, PQQ attenuates clinically relevant dysfunctions (e.g., those associated with ischemia, inflammation and lipotoxicity). PQQ is novel among biofactors that are not currently accepted as vitamins or conditional vitamins. For example, the absence of PQQ in diets produces a response like a vitamin–related deficiency with recovery upon PQQ repletion in a dose-dependent manner. Moreover, potential health benefits, such as improved metabolic flexibility and immuno-and neuroprotection, are associated with PQQ supplementation. Here, we address PQQ’s role as an enzymatic cofactor or accessory factor and highlight mechanisms underlying PQQ’s actions. We review both large scale and targeted datasets demonstrating that a neonatal or perinatal PQQ deficiency reduces mitochondria content and mitochondrial-related gene expression. Data are reviewed that suggest PQQ’s modulation of lactate acid and perhaps other dehydrogenases enhance NAD+–dependent sirtuin activity, along with the sirtuin targets, such as PGC-1α, NRF-1, NRF-2 and TFAM; thus, mediating mitochondrial functions. Taken together, current observations suggest vitamin-like PQQ has strong potential as a potent therapeutic nutraceutical.

show abstract

Glucose Dehydrogenase of Bacterium anitratum: an Enzyme with a Novel Prosthetic Group

Cited by 143 publications

References 18 publications

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Biooxidation with PQQ‐ and FAD‐Dependent Dehydrogenases

Pyrroloquinoline-Quinone Is More Than an Antioxidant: A Vitamin-like Accessory Factor Important in Health and Disease Prevention

Contact Info

Product

Resources

About