GLUCOSE CATABOLISM BY
            <i>BACILLUS POPILLIAE</i>
            AND
            <i>BACILLUS LENTIMORBUS</i>

Pepper, Rollin E.; Costilow, Ralph N.

doi:10.1128/jb.87.2.303-310.1964

Cited by 32 publications

(15 citation statements)

References 15 publications

(6 reference statements)

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“…The addition of buffer, MnSO4, CaC12, and DLtryptophan to 4% Trypticase did not have a highly significant effect, but barbituric acid greatly increased the percentage of cells developing refractile bodies. With use of this medium, a 20% inoculum of a 24 hr B-4 culture of either ) 2 6 8 B. popilliae or B. lentimorbus, incubated on a 2 4 6 8 shaker for 3 days at 30 to 32 C and allowed to IYS stand at 32 C for 7 to 14 days, resulted in a high illiae and B. lentimorpercentage of the cells (50 to 100%) forming Figure 4 shows clearly the morphological disit 0.1% glucose was tinction of the sporelike bodies produced in this 7ulation. Plate counts medium as compared with viable vegetative cells (24-hr culture) and with cells in 14-day B-4 broth FIG.…”

Section: Resultsmentioning

confidence: 99%

“…The rationale of the present study was that sporulation might occur in vitro if artificial media and cultural conditions were adjusted to permit the attainment of high populations of viable cells of B. popilliae and B. lentimorbus, providing that such cells retained their viability for a reasonable period of time. The extensive nutritional and metabolic studies reported previously (6,7,13) served as the primary basis for this investigation.…”

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confidence: 99%

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Production and Stabilization of Cells of Bacillus popilliae and Bacillus lentimorbus1

Costilow¹,

Sylvester²,

Pepper³

1966

Applied Microbiology

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Bacillus popilliae and B. lentimorbus grew most rapidly and to the greatest extent in aerated cultures at 30 to 32 C with oxygen absorption rates of 1 mmole of 02 per min per liter, or above. The control of pH also increased the maximal populations attained. Media were developed which consistently produced cell populations of about 109 within 24 to 48 hr in aerated cultures of these two species. The acetic acid produced in highly aerated cultures was shown not to be responsible for the rapid loss of cell viability in stationary phase cultures. However, H202 was very lethal to cells of B. popilliae, and this species is known to have the capacity to produce it. Stationaryphase cells were partially stabilized by reducing the availability of oxygen after 24 hr of incubation on a shaker, and the addition of low levels of glucose further stabilized the cells. The most stable cells were those produced in a medium in which 4% Trypticase (BBL) and 0.1 % barbituric acid were incorporated. A high percentage of these cells contained refractile bodies visible under a phase microscope. Although these bodies were not heat-resistant and lacked other characteristics of endospores, cells in cultures containing them had reasonably high viability for extended periods, as compared with those in control cultures.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Production and Stabilization of Cells of Bacillus popilliae and Bacillus lentimorbus1

Costilow¹,

Sylvester²,

Pepper³

1966

Applied Microbiology

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“…No close relationship was found between acetate-oxidizing ability and the sporeforming frequencies. Cells grown at 32 C failed to oxidize acetate but produced an appreciable number of spores in GYE medium; the opposite effect was observed with cells grown at 7 C. Cultures with the ability to actively oxidize acetate and with low sporeforming capacity at 7 C are rare among sporeforming organisms, although such organisms have been reported (4,23).…”

Section: Discussionmentioning

confidence: 99%

Influence of growth temperature on glucose metabolism of a psychotrophic strain of Bacillus cereus

Chung¹,

Cannon²,

Smith³

1976

Appl Environ Microbiol

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The influence of temperature on glucose metabolism of a psychrotrophic strain of Bacillus cereus was investigated. The pH of the growth medium and sporeforming frequencies of B. cereus varied when grown at 32, 20, or 7 C. Radiorespirometric analyses revealed that vegetative cells of B. cereus metabolized glucose by simultaneous operation of the Embden-Meyerhof-Parnas pathway and the pentose phosphate pathway. As the growth temperature decreased, glucose was metabolized with increased participation of the pentose phosphate pathway. The shift of cells grown at a higher temperature to a lower temperature increased the relative participation of the pentose phosphate pathway, whereas the shift of cells grown at low temperatures to a higher temperature had the opposite effect. Cells of late logarithmic phase grown at 20 and 7 C oxidized acetate by the tricarboxylic acid cycle reaction. However, cells grown at 32 C failed to oxidize acetate to CO2 to any appreciable extent. The extracellular products resulting from the metabolism of glucose decreased as the growth temperature was lowered. Organic acids were the major extracellular products of cultures grown at 32 and 20 C. Acetic acid, lactic acid, and pyruvic acid together accounted for 86.1 and 78.9%o of extracellular radioactivity, respectively, at the two temperatures. The relative ratio of these three acids varied between the temperatures. Little or no acid accumulated at 7 C.

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“…Acetate oxidation following vegetative proliferation is characteristic of other aerobic spore formers and is associated with their sporogenic processes (4). Of the strains of B. popilliae studied by Pepper and Costilow (6), only the single variant strain B-2309A oxidized acetate. Oxidation of acetate by this strain occurred only above pH 7 in an oxygen atmosphere.…”

Section: Fig 4 Effect Of Buffer Concentration On the Growthmentioning

confidence: 99%

Characteristics of the Vegetative Growth of Bacillus popilliae

Rhodes¹,

Sharpe²,

Hall³

et al. 1966

Applied Microbiology

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Growth characteristics of the insect pathogen, Bacillus popilliae Dutky, were studied by propagation in shaken flasks and in 2-liter fermentors. Maximal populations between 5 X 108 and 2 X 109 viable cells per milliliter of culture medium routinely were obtained in incubation periods of 18 to 24 hr at 30 C in a medium composed of 1.5% yeast extract, 0.6% K2HPO4, and 0.2% glucose or trehalose. The carbohydrate required for growth in liquid media was fermented with the formation of 2 meq of acid per mmole of carbohydrate utilized; acid products ordinarily were not subsequently metabolized. B. popilliae is an aerobe, and the amount of growth obtained varied with aeration to an optimum at oxygen absorption rates of about 0.5. Maximal populations persist in a culture for periods of only 1 to 4 hr; cessation of growth was followed immediately by rapid death of cultures, so that less than 1% of the cells remained viable after 48 hr, and viability often was lost entirely by the end of 72 hr of incubation. No cytological evidence for spore formation was observed under any growth condition. Death was not associated with lysis of the cells, although extensive granulation ultimately occurred. Continuous neutralizaiton, augmented buffering, various techniques of dialysis, or slow feeding of the carbohydrate did not markedly alleviate the characteristic death of the cultures.

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GLUCOSE CATABOLISM BY BACILLUS POPILLIAE AND BACILLUS LENTIMORBUS

Cited by 32 publications

References 15 publications

Production and Stabilization of Cells of Bacillus popilliae and Bacillus lentimorbus1

Production and Stabilization of Cells of Bacillus popilliae and Bacillus lentimorbus1

Influence of growth temperature on glucose metabolism of a psychotrophic strain of Bacillus cereus

Characteristics of the Vegetative Growth of Bacillus popilliae

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