Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Abstract

In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO(2)-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO(2)-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.

A sensitive glucose biosensor based on the abundant immobilization of glucose oxidase on hollow Pt nanospheres assembled on graphene oxide–Prussian Blue–PTC-NH2 nanocomposite film

“…In agreement with the previous studies [37,38], Fig. 2C showed both anodic and cathodic peak currents increased linearly with the square root of the scan rate over the range 30-250 mV/s, indicating that the biosensor exhibited diffusion-controlled behaviour.…”

“…5 shows that the current generated by interferents, such as ascorbic acid (AA), L-cysteine (L-cy), dopamine (DA) and ethanol (C 2 H 5 OH) could be ignored relative to the current attributed solely to glucose, which suggests that the prepared biosensor possessed an excellent anti-interference capacity. The good selectivity of the biosensor was ascribed to the low potential, which is similar to Zhang's report [37].…”

“…Reduction in the response at high pH was possibly due to the decrease of enzymatic activity. Therefore, the pH of the buffer solution was set to 6.0 in subsequent analyses, a similar result was obtained by Zhang et al [37].…”

Metal Oxide Nanoparticles in Electroanalysis

“…Metal oxide nanoparticles facilitate the third generation biosensors by assisting the immobilization of GOx onto the electrode for direct electron transfer between the electrode and GOx. Metal oxide nanoparticles that are most commonly used for the enzymatic detection of glucose are nickel oxide nanoparticles, iron oxide nanoparticles, and zinc oxide nanoparticles, even though other metal oxide nanoparticles have also been used to fabricate glucose biosensors, such as titanium oxide nanoparticles 10, 11, indium oxide nanoparticles 12, cobalt oxide nanoparticles 13, and even mixed oxide nanoparticles like cobalt ferrite nanoparticles 14.…”

Portable amperometric immunosensor for histamine detection using Prussian blue-chitosan-gold nanoparticle nanocomposite films

“…Prussian blue (PB) exhibits good electrocatalytic activity (W. Wang et al, 2014) and PB-based nanocomposites are primarily used for the immobilization of enzymes to fabricate an enzymatic biosensor (Zhang et al, 2011; Wang et al, 2012; B. Wang et al, 2014).…”