Glucose biosensor based on electrodeposition of platinum nanoparticles onto carbon nanotubes and immobilizing enzyme with chitosan-SiO2 sol–gel

Chitosan/siloxane hybrid polymer: synthesis, characterization and performance as a support for immobilizing enzyme

“…Incorporation of organic polymers, especially those with amino or amide groups, allows the formation of molecular hybrids often stabilized by strong hydrogen bonding. 15 Moreover, chemical additives can be used to improve the process and the attainment of materials with better mechanical properties, porosity control and hydrophilic/hydrophobic balance. 16 Several applications have been already developed for this kind of hybrid materials particularly in the biotechnological field.…”

“…15 This macromolecule, derived chemically by deacetylation of chitin, the second most abundant biopolymer in nature close to cellulose, has widely been assumed to be a cheaper and versatile sorbent for transition metal ions and organic substances through the coordination and/or reaction sites composed of the amino (-NH 2 ) and hydroxy (-OH) groups anchoring on chitosan chains. 20 As a matter of fact silica-chitosan possesses a highly porous microstructure and has been shown to be a superior support for immobilizing enzymes.…”

Fabrication of Pt Nanoparticles-decorated CVD Diamond Electrode for Biosensor Applications

“…In particular, platinum nanoparticles (Pt-NPs) exhibit excellent electrocatalytic properties for oxidation/reduction towards hydrogen peroxide (H2O2), a high surface-to-volume ratio, a high surface energy and efficient electron transfer between the redox proteins and electrode surface. 6 For these reasons, Pt-NPs modified electrodes are used frequently to detect a target analyte, H2O2, which is a product of the oxidation of substrates by the oxidoreductase enzyme in the presence of oxygen. 7 There are a few reports closely related with surface modification and functionalization of BDD electrodes for biosensors.…”

Influence of silane content on the optical properties of sol gel derived spin coated silica thin films

“…However, the usefulness of immobilized enzyme on electrodes depends on factors such as the immobilization method, the chemical and physical conditions (such as pH, temperature and contaminants), thickness and stability of the membrane used to couple the enzyme. Immobilization of enzyme in several matrices has been used for the fabrication of biosensors for estimation of glucose [3], lactate [4], cholesterol [5] etc. Various physical and chemical deposition techniques have been utilized to prepare thin films including chemical bath deposition, dip coating, Spray Pyrolysis Method and spin coating.…”