Glucose and lipid metabolic adaptations during postprandial starvation of Japanese flounder Paralichthys olivaceus previously fed different levels of dietary carbohydrates

Yang, Mingfang; Deng, Kailin; Pan, Mingzhu; Gu, Zhixiang; Liu, Dong; Zhang, Yue; Zhang, Wenbing; Mai, Kangsen

doi:10.1016/j.aquaculture.2018.12.003

Cited by 29 publications

(25 citation statements)

References 90 publications

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“…Table S2 (partially published) (Yang et al, ) shows the growth performance of Japanese flounder. After the 10‐week feeding trial, no significant difference was observed in the final body weight, survival rate (SR), feed efficiency ratio (FER), protein efficiency ratio (PER), specific growth rate (SGR) and feed intake (FI) among all the treatments ( p > .05).…”

Section: Resultsmentioning

confidence: 99%

“…The increase in dietary starch was concomitant with a decrease in carboxymethyl cellulose and bentonite clay. The formulation and proximate composition of the experimental diets referred to the previous study (Yang et al, ) and are shown in the Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…Total numbers were counted, and body weight of fish was measured. The growth performance of fish in C120 and C200 has been previously published (Yang et al, ). Before sampling, fish were anaesthetized with MS‐222 (Sigma, 50 mg/L water) and sacrificed by a sharp blow to the head.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma insulin was assayed by fish insulin ELISA kit (CSB‐E12123Fh) as described previously (Babaei, Abedian‐Kenari, Hedayati, & Yazdani‐Sadati, ). Glucagon level was measured using a commercially customized ELISA kit for fish according to the previous study (Yang et al, ). Plasma leptin was measured by fish leptin ELISA kit (CSB‐EL012870FI) (Kousoulaki et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

et al. 2019

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Japanese flounder juveniles (initial body weight: 7.12 ± 0.02 g) were fed three diets containing 0, 120 and 200 g/kg of carbohydrates (C0, C120 and C200, respectively) for 10 weeks. Results showed that higher dietary carbohydrate intake enabled further deposition of glycogen and lipids in liver and muscle. The mRNA levels of glucokinase (gck), phosphofructokinase (pfkl) and hexokinase (HK) activity involved in glycolysis were significantly up‐regulated in C120 (p < .05) but showed no further up‐regulation except for gck in C200. Besides, the gluconeogenic phosphoenolpyruvate carboxykinase 1 (pck1) mRNA levels were down‐regulated significantly in fish fed the C120 (p < .05). However, further increase in dietary carbohydrate levels increased the mRNA levels and activities of enzymes involved in gluconeogenesis and lipolysis (p < .05). Additionally, plasma glucose remained unchanged in C120 (p > .05) but significantly increased in C200 group (p < .05). In conclusion, Japanese flounder was able to use carbohydrates efficiently through regulation of glucose and lipid metabolism when dietary carbohydrate was not higher than 120 g/kg, while 200 g/kg of dietary carbohydrate caused the deregulation of glucose homoeostasis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

et al. 2019

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“…Previous studies found that high dietary carbohydrate level led to growth inhibition and inflammation in olive flounder. Companied with these changes, one of the main physiological indicators in the olive flounder fed with high carbohydrate diet is the significantly higher blood glucose level compared with the fish fed with lower carbohydrate diet (17,54,55) . In the present study, 33mM of medium glucose concentration led to a significant decrease in cell viability, which is a critical in vitro Accepted manuscript…”

Section: Discussionmentioning

confidence: 99%

High glucose induces apoptosis, glycogen accumulation and suppresses protein synthesis in muscle cells of olive flounder Paralichthys olivaceus

et al. 2021

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The effect and the mechanism of high glucose on fish muscle cells are not fully understood. In the present study, muscle cells of olive flounder (Paralichthys olivaceus) were treated with high glucose (33 mM) in vitro. Cells were incubated in three kinds of medium containing 5 mM glucose, 5 mM glucose and 28 mM mannitol (as an isotonic contrast) or 33 mM glucose named the Control group, the Mannitol group and the HG (high glucose) group, respectively. Results showed that high glucose increased the ADP/ATP ratio and the reactive oxygen species (ROS) level, decreased mitochondrial membrane potential (MMP), induced the release of cytochrome C (CytC) and cell apoptosis. High glucose also led to cell glycogen accumulation by increasing the glucose uptake ability and affecting the mRNA expressions of glycogen synthase and glycogen phosphorylase. Meanwhile, it activated AMP-activated protein kinase (AMPK), inhibited the activity of mammalian target of rapamycin (mTOR) signaling pathway and the expressions of myogenic regulatory factors (MRFs). The expressions of myostatin-1 (mstn-1) and E3 ubiquitin ligases including muscle RING-finger protein 1 (murf-1) and muscle atrophy F-box protein (mafbx) were also increased by the high glucose treatment. No difference was found between the Mannitol group and the Control group. These results demonstrate that high glucose has the effects of inducing apoptosis, increasing glycogen accumulation and inhibiting protein synthesis on muscle cells of olive flounder. The mitochondria mediated apoptotic signaling pathway, AMPK and mTOR pathways participated in these biological effects.

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Carbohydrate Preference and Metabolism—‘Life’s Little Luxury Digested’

Steinberg

2022

Aquatic Animal Nutrition

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Glucose and lipid metabolic adaptations during postprandial starvation of Japanese flounder Paralichthys olivaceus previously fed different levels of dietary carbohydrates

Cited by 29 publications

References 90 publications

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

High glucose induces apoptosis, glycogen accumulation and suppresses protein synthesis in muscle cells of olive flounder Paralichthys olivaceus

Carbohydrate Preference and Metabolism—‘Life’s Little Luxury Digested’

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