Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens

Hutchison, Victor H.; Turney, L. Douglas

doi:10.1007/bf00710369

Cited by 45 publications

(13 citation statements)

References 19 publications

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“…The sampled structure had a milky appearance that was consistent with earlier descriptions of the endolymphatic system. Both exercise and anoxia increased lactate concentrations in all tissues examined to levels observed in previous studies (Andersen and Wang, 2003;Armentrout and Rose, 1971;Bennett and Licht, 1974;D'Eon et al, 1978;Donohoe and Boutilier, 1999;Hutchison and Turney, 1975;Warburton et al, 1989;Wasser et al, 1993Wasser et al, , 1991Wegener and Krause, 1993). With the exception of gastrocnemius and heart, plasma had a higher lactate concentration than the other tissues examined, as has also been observed in cold hypoxic frogs (Donohoe and Boutilier, 1999) and cold anoxic turtles (Jackson et al, 1996).…”

Section: Bone Incubationssupporting

confidence: 67%

The role of mineralized tissue in the buffering of lactic acid during anoxia and exercise in the leopard frogRana pipiens

Warren

Jackson

2005

Journal of Experimental Biology

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SUMMARY To evaluate the role of mineralized tissues of the leopard frog in buffering acid, we analyzed the composition of femur and auditory capsule, the latter of which encloses a portion of the endolymphatic lime sacs, and investigated the extent to which these tissues are involved in buffering lactic acid after 2.5 h of anoxia and 10-19 min of strenuous exercise at 15°C. We analyzed the following tissues for lactate: plasma, heart, liver,gastrocnemius muscle, femur, auditory capsule and carcass. Plasma[Ca2+], [Mg2+], [inorganic phosphate (Pi)],[Na+] and [K+] were also measured. Femur Ca2+, Pi and CO32- compositions were similar to bone in other vertebrates. Auditory capsule had significantly more CaCO3 than femur. Lactate was significantly elevated in all tissues after anoxia and exercise, including femur and auditory capsule. Anoxia increased plasma [Ca2+], [Mg2+], [Pi]and [K+] and had no effect on plasma [Na+]. Exercise increased plasma [Mg2+], [Pi] and [K+] and had no effect on plasma [Ca2+] or [Na+]. The skeleton and endolymphatic lime sacs buffered 21% of the total lactate load during anoxia, and 9% after exercise. The exact contribution of the entire endolymphatic sac system to lactate buffering could not be determined in the present study. We conclude that the mineralized tissues function as buffers during anoxia and exercised induced lactic acidosis in amphibians.

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Section: Bone Incubationssupporting

confidence: 67%

The role of mineralized tissue in the buffering of lactic acid during anoxia and exercise in the leopard frogRana pipiens

Warren

Jackson

2005

Journal of Experimental Biology

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“…Wang & Chang (1994) found glucose levels varying between 20 and 64mg/dL, and Stefani (1996) values from 48.97 to 55.86mg%. Hutchison & Turkey (1975) determined mean blood glucose levels of 24.5±3.0mg% for Rana pipiens, while Smith (1954) observed in Rana temporaria mean values of 38±1.4mg%. Baseline levels of blood glucose found in the present study varied between 49.33 and 104.33mg/dL (76.07±18.50mg/dL).…”

Section: Resultsmentioning

confidence: 91%

Physiological response of American bullfrog tadpoles to stressor conditions of capture and hypoxia

Rocha¹,

Ferreira²,

Teixeira

et al. 2010

Pesq. Vet. Bras.

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RESUMO.-[Resposta fisiológica de girinos de rã-touro americana submetidos aos mecanismos estressores de captura e hipóxia.] A rã-touro americana (Rana catesbeiana) recentemente denominada Lithobates catesbeianus é criada com propósito comercial em várias regiões do Brasil. Situações estressantes tais como problemas de manejo, criação inadequada e alterações ambientais com consequente redução da imunidade são comuns em produções intensivas. A avaliação destas situações de estresse permitenos detectar estes probemas e diminuir as injurias causadas pelo confinamento. O principal objetivo deste estudo foi utilizar os marcadores biológicos de cortisol, glicemia e dados hematológicos para avaliar a resposta de girinos de rã-touro submetidos aos mecanismos estressores de captura e hipóxia. Os animais foram distribuídosem três tratamentos: estresse por captura individual com puçá; estresse por captura em massa com puçá e estresse por captura por escoamento. Os resultados obtidos demostraram não haver diferenças estatisticamente significativas entre os parametros avaliados quando comparou-se os grupos com e sem exposição ao ar (normoxia e hipoxia). Com base nes- Stressful situations such as problems of management, inadequate facilities and environmental changes with consequent reduction of immunity are common in intensive production. The assessments of these situations of stress allow us detect these problems decreasing the injuries causes by confinement. The main objective of this study was to use the biological markers of plasma cortisol and glucose level and hematological parameters to evaluate the response of bullfrog tadpoles submitted to stressed mechanisms of capture and hypoxia. The animals were subjected to three treatments: stress due to individual capture with a hand net; stress due to batch capture with a hand net; and stress due to capture by emptying. The results obtained demonstrated that there were no statistically significant differences in the parameters tested when comparing the treatments with and without exposure to air (normoxia and hypoxia). Based on these results we can conclude that the stressful stimuli tested were not adequate to alter the biomarker tested. For the cortisol, probably this should have occurred due to the synergistic action between this hormone and thyroxin, which induces metamorphosis in these animals. tes resultados pode-se concluir que os estímulos estressores avaliados não foram adequados para alterar os valores plasmáticos dos marcadores biológicos testados. Para o cortisol, isto ocorreu provavelmente em virtude da ação sinérgica deste hormônio e a tiroxina, que induz a metamorfose nestes animais.

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“…Establishing reference intervals in reptiles and amphibians is complicated by difficulties associated with specimen collection and variations in analytes related to age, sex, nutritional status, health status, temperature, season, handling, and analytical methods . Several biochemical analytes, including plasma protein, sodium, calcium, glucose, and uric acid concentrations, have been reported to vary widely depending on intrinsic and extrinsic factors . Plasma potassium concentration was relatively stable throughout the year in one anuran species …”

Section: Introductionmentioning

confidence: 99%

Hematologic and plasma biochemical reference intervals for health monitoring of wild Australian tree frogs

Young¹,

Warner

Speare³

et al. 2012

Veterinary Clinical Pathol

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Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens

Cited by 45 publications

References 19 publications

The role of mineralized tissue in the buffering of lactic acid during anoxia and exercise in the leopard frogRana pipiens

The role of mineralized tissue in the buffering of lactic acid during anoxia and exercise in the leopard frogRana pipiens

Physiological response of American bullfrog tadpoles to stressor conditions of capture and hypoxia

Hematologic and plasma biochemical reference intervals for health monitoring of wild Australian tree frogs

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