Glucose-6P dehydrogenase in Chlorella sorokiniana (211/8k): an enzyme with unusual characteristics

Esposito, Sergio; Guerriero, Gea; Vona, Vincenza; Rigano, Vittoria Di Martino; Carfagna, Simona; Rigano, Carmelo

doi:10.1007/s00425-005-0110-2

Cited by 13 publications

(6 citation statements)

References 33 publications

(69 reference statements)

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“…This result may be due to the lower concentration of interfering compounds (e.g., proteins) present in microalgae grown under nitrogen starvation conditions. It has been reported (Esposito et al 2006) that the protein content in green microalgae is about threefold higher in nitrogensufficient cells with respect to nitrogen-starved cells. According to Chow and Landhäusser (2004), watersoluble compounds such as proteins react with the concentrated sulfuric acid in the glucose assay and, therefore, significantly interfere with the absorbance reading.…”

Section: Resultsmentioning

confidence: 99%

Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods

et al. 2011

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Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher than those estimated by using hydrochloric acid as solubilizing and hydrolyzing agent. The enzymatic method (EM1) proved to be the most rapid and precise method for microalgal starch quantification. Furthermore, the evaluation of resistant starch by enzymatic methods assayed in nitrogen-sufficient and nitrogen-starved cells showed that no formation of this type of starch occurred in microalgae, meaning that this should not interfere with starch content determinations.

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Section: Resultsmentioning

confidence: 99%

Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods

et al. 2011

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“…Esposito et al. have shown that in nitrate fed barley seedlings, GOGAT activity was at the maximum after 3 days, whereas in ammonium supplied plants, the highest rates of GOGAT activity were observed after 1 day.…”

Section: Discussionmentioning

confidence: 99%

Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen‐starved wheat seedlings

Balotf

Kavoosi

Kholdebarin

2015

Biotech and App Biochem

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The objective of this study was to examine the expression and activity of nitrate reductase (NR, EC 1.7.1.1), nitrite reductase (NiR, EC 1.7.2.2), glutamine synthetase (GS, EC 6.3.1.2), and glutamate synthase (GOGAT, EC 1.4.7.1) in response to potassium nitrate, ammonium chloride, and ammonium nitrate in nitrogen-starved wheat seedlings. Plants were grown in standard nutrient solution for 17 days and then subjected to nitrogen starvation for 7 days. The starved plants were supplied with potassium nitrate ammonium nitrate and ammonium chloride (50 mM) for 4 days and the leaves were harvested. The relative expression of NR, NiR, GS, and GOGAT as well as the enzyme activities were investigated. Nitrogen starvation caused a significant decrease both in transcript levels and in NR, NiR, GS, and GOGAT activities. Potassium nitrate and ammonium nitrate treatments restored NR, NiR, GS, and GOGAT expressions and activities. Ammonium chloride increased only the expressions and activities of GS and GOGAT in a dose-dependent manner. The results of our study highlight the differential effects between the type and the amount of nitrogen salts on NR, NiR, GS, and GOGAT activities in wheat seedlings while potassium nitrate being more effective.

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“…The NADH-GOGAT (EC. 1.4.1.14) and Fd-GOGAT (EC 1.4.7.1) activities were detected, as described by Esposito et al [52].…”

Section: Enzymatic Activities Assaymentioning

confidence: 55%

Nitrogen Metabolism at Tillering Stage Differently Affects the Grain Yield and Grain Protein Content in Two Durum Wheat Cultivars

et al. 2019

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Soil nitrogen abundance, as well as nitrogen use efficiency (NUE), significantly affect the crop yield and grain protein content (GPC). Depending on the genotype, a negative correlation between the yield and GPC can occur. The aim of the study was to assess the agronomic performance, and to explore physiological pathways for the efficient use of N fertilizer for two durum wheat cultivars, “Aureo” and “Vespucci”. After fertilization, the nitrogen content and values of some of the agronomic parameters and yield-related traits increased in both cultivars; nevertheless, a simultaneous rise in both the yield and GPC occurred only in Aureo. The biochemical parameters, analyzed at tillering, confirm the genotypic specificity of nitrogen use. In Vespucci’s roots, the nitrogen supply did not affect the nitrate reductase (NR), but greatly increased the amino acids and proteins, suggesting that ammonium is preferentially assimilated. In Aureo, nitrate is in part assimilated by the roots, as suggested by the ammonium increase and NR enhancement. In the leaves of both cultivars, organic nitrogen significantly increased after fertilization; however, the rise in amino acids, as well as in NR activity, was higher in Aureo than in Vespucci. These results indicate that the different nitrogen use, and in particular the diverse NR behavior, at tillering, are in part responsible of the cultivar differences in grain yield and GPC.

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Glucose-6P dehydrogenase in Chlorella sorokiniana (211/8k): an enzyme with unusual characteristics

Cited by 13 publications

References 33 publications

Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods

Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods

Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen‐starved wheat seedlings

Nitrogen Metabolism at Tillering Stage Differently Affects the Grain Yield and Grain Protein Content in Two Durum Wheat Cultivars

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