Glucose-6-Phosphatase Histochemistry of Osteoarthritis.

Inaba, Takashi; Kariya, Yutaka; Hoshino, Yuichi; Ooi, Yoshio; Yashiro, Takashi; Saito, Takuma

doi:10.1267/ahc.30.93

Cited by 3 publications

(5 citation statements)

References 22 publications

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“…The positive activity in the Golgi cisternae, which is in good accordance with the biochemical report, may indicate these regulatory mechanisms on the secretory and/or endocytotic activities, as the B cells active in the carbohydrate metabolism for the production of synovial fluid [19]. GCase localization was also different from ACase, showing rather weak activity on the plasma membranes of the A cells, and also strong activity on the pinocytotic vesicles in the B cells.…”

Section: Discussionsupporting

confidence: 86%

“…In this experimental osteoarthritis the surface synovial cells showed a thikening up to 5-6 cell layers and an increase in fibroblasts and capillaries such as are concomitantly observed in human osteoarthritis [19].…”

Section: Discussionsupporting

confidence: 70%

“…Investigations using cytochemical techniques are useful for the study of tissues consisting of many kinds of cells [16,26,33]. However, most past studies have been limited to light microscopy so that the ultrastructural cytochemistry is limited [19,25]. The purpose of this report is to describe the cyclic nucleotide metabolizing enzymes, such as adenylate cyclase (ACase) [42], guanylate cyclase (GCase) [43], and 5'-nucleotidase (5'-N) [47], by focusing on the function of the synovial membrane in osteoarthritis cytochemically.…”

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confidence: 99%

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Histochemistry of Cyclic Nucleotide Metabolizing Enzymes in Osteoarthritis.

Inaba

Kariya

Hoshino

et al. 1997

Acta Histochem. Cytochem

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 70%

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confidence: 99%

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Histochemistry of Cyclic Nucleotide Metabolizing Enzymes in Osteoarthritis.

Inaba

Kariya

Hoshino

et al. 1997

Acta Histochem. Cytochem

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“…For example, glucose-6-phosphatase enzymehistochemistry revealed distinct cell populations in rat synovial membrane cells (Inaba et al 1997). These cells (type III synovial cells) showed a characteristic staining pattern for this enzyme, which has never been shown by ordinary TEM morphological analysis without enzymehistochemistry.…”

Section: Discussionmentioning

confidence: 99%

Human amniotic epithelial cells are morphologically homogeneous: enzymehistochemical, tracer, and freeze-substitution fixation study

Iwasaki

Matsubara²,

Tajima³

et al. 2009

Eur J Histochem

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We examined the fine subcellular morphology of human amniotic epithelial cells and attempted to answer the question as to whether amniotic epithelial cells consist of heterogeneous or homogeneous cells, which has long been controversial. Study subjects were fetal membranes from pregnant women (n=18) who abdominally gave birth to healthy infants at term (37.9±0.7 weeks of gestation, mean±sd). The methods employed were transmission electron microscopy, enzymehistochemistry, tracer permeability analysis, and freeze-substitution fixation. The labelings for acid phosphatase, cytochrome c oxidase, and CA ++ ATPase were seen in the lysosomes, mitochondria, and lateral plasma membranes, respectively. The staining distribution pattern of these three enzymes and the morphology of the organelle highlighted by these enzymehistochemistry did not differ among cells. Freeze-substitution fixation revealed that intercellular spaces in the amniotic epithelial cells were narrower than previously thought, but the tracers (horse radish peroxidase and lanthanum nitrate) fully entered these spaces. There were no variations in the tracer permeability among cells. All cells from freeze-substitution fixation exhibited the same morphological features. From these morphological viewpoints, we conclude that human term amniotic epithelial cells consist of a homogeneous cell population.

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“…Quite a similar pattern of change in mitochondrial morphology was observed during the dif- ivs Intervillous space. For example, Inaba et al (1997) demonstrated that the ER number per unit area significantly differed between two different cell types in rat synovial membranes, which reflected different functional aspects of these two cells. Diaminobenzidine deposits indicative of cytochrome c oxidase activity were visible in the intermembrane spaces and intracristal spaces of the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Morphology of the mitochondria and endoplasmic reticula of chorion laeve cytotrophoblasts: their resemblance to villous syncytiotrophoblasts rather than villous cytotrophoblasts

Matsubara

Takayama

Iwasaki

et al. 2001

Histochem Cell Biol

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We examined the morphological features of the mitochondria and endoplasmic reticula of chorion laeve cytotrophoblasts from term human fetal membranes, and compared them with those of syncytiotrophoblasts and cytotrophoblasts from human placental villi. Ultrastructural enzyme histochemistry of cytochrome c oxidase and glucose-6-phosphatase were used as cytochemical markers for these intracellular organelles. Chorion laeve cytotrophoblasts possessed abundant endoplasmic reticula, and small mitochondria with a few cristae, which were characteristic of villous syncytiotrophoblasts rather than villous cytotrophoblasts. As for these organellar structures, statistical analysis confirmed similarities between chorion laeve cytotrophoblasts and villous syncytiotrophoblasts, but significant differences between laeve cytotrophoblasts and villous cytotrophoblasts. Though these two cytotrophoblasts originated from one common cell in early placental development, they exhibited quite different organellar morphology during placental/chorioamniotic differentiation. Considering previous data, we concluded that chorion laeve cytotrophoblasts were metabolically active cells, similar to villous syncytiotrophoblasts, performing many functions in fetal membrane physiology.

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Glucose-6-Phosphatase Histochemistry of Osteoarthritis.

Cited by 3 publications

References 22 publications

Histochemistry of Cyclic Nucleotide Metabolizing Enzymes in Osteoarthritis.

Histochemistry of Cyclic Nucleotide Metabolizing Enzymes in Osteoarthritis.

Human amniotic epithelial cells are morphologically homogeneous: enzymehistochemical, tracer, and freeze-substitution fixation study

Morphology of the mitochondria and endoplasmic reticula of chorion laeve cytotrophoblasts: their resemblance to villous syncytiotrophoblasts rather than villous cytotrophoblasts

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