Glucose 6 phosphatase dehydrogenase (G6PD) and neurodegenerative disorders: Mapping diagnostic and therapeutic opportunities

Tiwari, Manju

doi:10.1016/j.gendis.2017.09.001

Cited by 55 publications

(41 citation statements)

References 67 publications

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“…Three different markers, known to increase under stress conditions, were measured to estimate the stress response of skin ( Figure 3 A). G6PDH was used as a marker for cell metabolism [ 26 , 27 ], loricrin for cell differentiation [ 28 , 29 ], and γH2AX for DNA damage [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

A Rose Extract Protects the Skin against Stress Mediators: A Potential Role of Olfactory Receptors

et al. 2020

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Olfactory receptors (ORs) are expressed and active in various human tissues, including the skin. Although the sense of smell plays an important physiological role in the regulation of mood and stress, a link between olfactive compounds, ORs, and skin stress has yet to be established. This study aims to investigate the role of newly identified skin ORs and agonists in the modulation of skin stress. Screening for odorant molecules was done with cAMP functional assay to identify OR agonists. RT-qPCR and immunofluorescence microscopy were conducted to identify and quantify ORs in epidermal keratinocytes (NHEKs) and human skin explants, as well as to evaluate specific markers (G6PDH, loricrin, and γH2AX) of stress-induced skin alterations. A randomized double-blinded, split-face clinical study was performed on a panel of stressed women to measure the benefits of OR agonist treatment for skin. Three new ORs (OR10A6, OR2AG2, and OR11H4) were identified in skin. A specific Rose extract and its major constituent (phenylethyl alcohol) were found to activate these ORs. The extract composition was revealed by both GC/FID and GC/MS analyses simultaneously and showed the presence of 34 volatiles molecules. Moreover, epinephrine induces a skin stress response characterized by increased expression of G6PD, loricrin, and γH2AX biomarkers, and a decrease of OR expression. These effects were prevented in the presence of rose extract and its benefits were confirmed clinically by a decrease in the appearance of under-eye dark circles. Altogether, our findings suggest that ORs may represent a new, promising way to treat stress-associated skin disorders.

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Section: Resultsmentioning

confidence: 99%

A Rose Extract Protects the Skin against Stress Mediators: A Potential Role of Olfactory Receptors

et al. 2020

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“…G6PD, an enzyme of the pentose phosphate shunt, produces the reducing NADPH for GSH generation [54]. This enzyme activity is increased in the brain of experimental animals treated with Al [55,56]. Increased G6PD activity indicates oxidation of glucose through the hexose monophosphate pathway, thereby facilitating increased production of NADPH for detoxification process.…”

Section: Discussionmentioning

confidence: 99%

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

Karabulut-Bulan¹,

Bayrak²,

Sarikaya-Unal³

et al. 2019

jrp

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Aluminum (Al), an ubiquitous element in nature, enters the body primarily through gastrointestinal tract, respiratory system and skin. Being a powerful neurotoxin for human brain, Al was reported to be involved in the etiology of Alzheimer's disease due to its easy access and accumulation in the central nervous system. Melatonin (Mel) is a tryptophan-derived neurohormone in animals and plants, and produced in the pineal gland of all mammalian species. The present study examines the effects of Mel on Al-induced oxidative stress, inflammation, tissue factor production and brain damage in rat brain. Wistar albino rats were divided into four groups. Group I: control animals; Group II: rats injected with 10 mg/kg Mel; Group III: rats injected with 5 mg/kg Al2(SO4)3; and Group IV: rats injected combination of Al and Mel (5 mg/kg Al2(SO4)3 and 10 mg/kg Mel). Animals were injected three times a week for one month. At the end of the month, rats were sacrificed, their brains were removed. It was found that lipid peroxidation, protein carbonyl, advanced oxidation protein products, hydroxyproline levels, tissue factor, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, myeloperoxidase, acetylcholine esterase, alkaline phosphatase, acid phosphatase and glucose-6-phosphate dehydrogenase activities were increased, while paraoxonase, arylesterase, sodium potassium ATPase activities and glutathione levels were decreased in the Al-treated group. Mel treatment reversed these changes by demonstrating significant antioxidant effects. Results indicated that Mel has potential therapeutic value against Al-induced oxidative stress in the rat brain tissue and these effects may be related to its antioxidant activities.

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“…Authors' information 1 Oxidation in Red Cell Disorders Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Thailand.…”

Section: Fundingmentioning

confidence: 99%

Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

Sanephonasa

Cheepsunthorn

Khaminsou

et al. 2020

Preprint

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Background: The prevalence and genotypes of G6PD deficiency vary worldwide, with higher prevalence in malaria endemic areas. The first time assessment of G6PD deficiency prevalence and molecular characterization of G6PD mutations in the Lao Theung population were performed in this study.Methods: A total of 252 unrelated Lao Theung participants residing in the Lao People's Democratic Republic (PDR) were recruited. All participant samples were tested for G6PD enzyme activity and G6PD gene mutations. The amplification refractory mutation system (ARMS)-PCR for detecting G6PD Aures was developed.Results: The G6PD mutations were detected in 11.51% (29/252) of the participants. Eight G6PD mutations were detected. The G6PD Aures was the most common mutation identified in this cohort, which represented 58.62 % (17/29) of all mutation. The mutation pattern was homogenous, predominantly involving the G6PD Aures mutation (6.75%), followed by 1.19% G6PD Union and 0.79% each G6PD Jammu, G6PD Mahidol and G6PD Kaiping. One subject (0.4%) each carried G6PD Viangchan and G6PD Canton. Interestingly, one case of coinheritance of G6PD Aures and Quing Yan was detected in this cohort. Based on levels of G6PD enzyme activity, the prevalence of G6PD deficiency in the Lao Theung population was 9.52 % (24/252).Conclusion: The G6PD Aures mutation is highly prevalent in the Lao Theung ethnic group. The common G6PD variants in continental Southeast Asian populations, G6PD Viangchan, Canton, Kaiping, Union and Mahidol, were not prevalent in this ethnic group. The technical simplicity of the developed ARMS-PCR will facilitate the final diagnosis of the G6PD Aures.

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Glucose 6 phosphatase dehydrogenase (G6PD) and neurodegenerative disorders: Mapping diagnostic and therapeutic opportunities

Cited by 55 publications

References 67 publications

A Rose Extract Protects the Skin against Stress Mediators: A Potential Role of Olfactory Receptors

A Rose Extract Protects the Skin against Stress Mediators: A Potential Role of Olfactory Receptors

The influence of melatonin supplementation against aluminum-induced toxicity in brains of male rats

Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

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