Glucose-6-phosphatase and age: biochemical and histochemical studies

Plewka, Andrzej; Kamiński, Marian; Plewka, Danuta; Nowaczyk, Grażyna

doi:10.1016/s0047-6374(99)00097-4

Cited by 7 publications

(1 citation statement)

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“…The number of positively stained epithelial cells for B2R and B1R in eight different areas (173 ϫ 130 m) of each tissue sample was determined using computer-assisted image analysis. In each enterocyte, the intensity of staining was measured as the optical density of the reaction product, as described previously (13), with the program KS 100 VIDAS video image analyzer served by IBAS 2.5 system and a Panasonic WV-CL700 digital camera (Kontron Elektronic, Warsaw, Poland) at a magnification of 25-fold (10,11,21). For each analyzed area, 173 ϫ 130 m average optical density per unit area was calculated.…”

Section: Methodsmentioning

confidence: 99%

Immunolocalization and expression of kinin B₁R and B₂R receptors in human inflammatory bowel disease

Stadnicki

Pastucha²,

Nowaczyk³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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Bradykinin is a mediator of inflammation, responsible for pain, vasodilation, and capillary permeability. Bradykinin receptor 1 (B1R) and bradykinin receptor 2 (B2R) are G protein-coupled receptors that mediate kinin effects. The latter is constitutive and rapidly desensitized; the former is induced by inflammatory cytokines and resistant to densensitization. The distribution of bradykinin receptors in human intestinal tissue was studied in patients with inflammatory bowel disease (IBD), namely ulcerative colitis (UC) and Crohn's disease (CD). Both B2R and B1R proteins are expressed in the epithelial cells of normal and IBD intestines. B1R protein is visualized in macrophages at the center of granulomas in CD. B2R protein is normally present in the apexes of enterocytes in the basal area and intracellularly in inflammatory tissue. In contrast, B1R protein is found in the basal area of enterocytes in normal intestine but in the apical portion of enterocytes in inflamed tissue. B1R protein is significantly increased in both active UC and CD intestines compared with controls. In patients with active UC, B1R mRNA is significantly higher than B2R mRNA. However, in inactive UC patients, the B1R and B2R mRNA did not differ significantly. Thus bradykinin receptors in IBD may reflect intestinal inflammation. Increased B1R gene and protein expression in active IBD provides a structural basis of the important role of bradykinin in chronic inflammation.

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Section: Methodsmentioning

confidence: 99%