Glucosamine-NISV delivers antibody across the blood-brain barrier: Optimization for treatment of encephalitic viruses

Woods, Stuart; O’Brien, Lyn M.; Butcher, Wendy; Preston, Jane E.; Georgian, Ana Ruxandra; Williamson, E. Diane; Salguero, Francisco J.; Modino, Francesca; Abbott, N. Joan; Roberts, Craig W.; D’Elia, Riccardo V.

doi:10.1016/j.jconrel.2020.05.048

Cited by 13 publications

(2 citation statements)

References 44 publications

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“…Thus, designing targeted NPs to increase the brain entry of drugs across the BBB, called as Trojan horse mechanism, was the main focus of NP research in the last decade . A wide variety of ligands or antibodies specific to BBB receptors − or carriers − were tested on cell culture models and in animals as promising targeting molecules to elevate the efficiency of BBB crossing. Among them, the tripeptide glutathione (GSH) is one of the most successful BBB-specific targeting ligands .…”

Section: Introductionmentioning

confidence: 99%

Optically Manipulated Microtools to Measure Adhesion of the Nanoparticle-Targeting Ligand Glutathione to Brain Endothelial Cells

Fekete

Mészáros

Szegletes

et al. 2021

ACS Appl. Mater. Interfaces

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Targeting nanoparticles as drug delivery platforms is crucial to facilitate their cellular entry. Docking of nanoparticles by targeting ligands on cell membranes is the first step for the initiation of cellular uptake. As a model system, we studied brain microvascular endothelial cells, which form the anatomical basis of the blood−brain barrier, and the tripeptide glutathione, one of the most effective targeting ligands of nanoparticles to cross the blood−brain barrier. To investigate this initial docking step between glutathione and the membrane of living brain endothelial cells, we applied our recently developed innovative optical method. We present a microtool, with a task-specific geometry used as a probe, actuated by multifocus optical tweezers to characterize the adhesion probability and strength of glutathione-coated surfaces to the cell membrane of endothelial cells. The binding probability of the glutathione-coated surface and the adhesion force between the microtool and cell membrane was measured in a novel arrangement: cells were cultured on a vertical polymer wall and the mechanical forces were generated laterally and at the same time, perpendicularly to the plasma membrane. The adhesion force values were also determined with more conventional atomic force microscopy (AFM) measurements using functionalized colloidal probes. The optical trapping-based method was found to be suitable to measure very low adhesion forces (≤ 20 pN) without a high level of noise, which is characteristic for AFM measurements in this range. The holographic optical tweezers-directed functionalized microtools may help characterize the adhesion step of nanoparticles initiating transcytosis and select ligands to target nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Optically Manipulated Microtools to Measure Adhesion of the Nanoparticle-Targeting Ligand Glutathione to Brain Endothelial Cells

Fekete

Mészáros

Szegletes

et al. 2021

ACS Appl. Mater. Interfaces

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“…В последние годы интерес к ресурсосберегающим процессам синтеза различных химических соединений, а в частности к реакции восстановительного аминирования сильно возрос [1-3]. Это связано с тем, что продуктом восстановительного аминирования D-глюкозы и метиламина является N-метилглюкамин, широко применяемый в медицинской химии для высокоэффективного повышения солюбилизации и стабилизации биологически активных соединений [4], используемых при лечении вирусных заболеваний [5], для лечения проблем опорно-двигательной системы человека [6], при терапии онкологических заболеваний [7], а так же используется как катализатор для [8,9]. Для увеличения скорости процесса синтеза, активности и селективности по отношению к целевому продукту, некоторые из образцов катализаторов приходится модифицировать [10,11].…”

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Modification of Nickel Catalysts for Reductive Amination Based on Polymer Supports

Михаилов¹,

Долуда²,

Sulman³

et al. 2022

Вестник Тверского Государственного Университета. Серия: Химия

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Процесс каталитической гидроконденсации является основным для синтеза огромного количества химических соединений. Примером таких соединений могут являться N-содержащие функциональные соединения, которые можно использовать в качестве исходного продукта для получения фармацевтических препаратов и агрохимикатов. За последние годы было проделано не мало исследований по поиску наиболее активного и селективного гетерогенного катализатора реакции восстановительного аминирования. Настоящая статья посвящена изучению Ni - содержащих катализаторов на основе полимерных носителей и результатам их модификации. The process of catalytic hydrocondensation is the main one for the synthesis of a huge number of chemical compounds. An example of such compounds can be N-containing functional compounds that can be used as a starting product for the production of pharmaceuticals and agrochemicals. In recent years, a lot of research has been done to find the most active and selective heterogeneous catalyst for the reductive amination reaction. This article is devoted to the study of Ni-containing polymer-supported catalysts and the results of their modification.

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Anatomy and Physiology of the Blood-Brain Barriers*

Abbott

2022

Drug Delivery to the Brain

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Glucosamine-NISV delivers antibody across the blood-brain barrier: Optimization for treatment of encephalitic viruses

Cited by 13 publications

References 44 publications

Optically Manipulated Microtools to Measure Adhesion of the Nanoparticle-Targeting Ligand Glutathione to Brain Endothelial Cells

Optically Manipulated Microtools to Measure Adhesion of the Nanoparticle-Targeting Ligand Glutathione to Brain Endothelial Cells

Modification of Nickel Catalysts for Reductive Amination Based on Polymer Supports

Anatomy and Physiology of the Blood-Brain Barriers*

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