Gluconobacter sp. cells for manufacturing of effective electrochemical biosensors and biofuel cells

Abstract

Gluconobacter oxydans bacteria exhibit a unique metabolism for quick and incomplete oxidation of a wide range of different compounds (aldoses, ketoses, mono- and poly-alcohols, etc.). Such biotransformation efficiency with simple biomass production led to the industrial applications of these bacteria in the production of several important commodities. Their respiratory activity can also be successfully studied and used in the field of bioelectrochemistry. The main aim of this review is to present various strategies to improve selectivity of assays using intact/treated cells of G. oxydans, to introduce the application of G. oxydans-based biosensors in selective monitoring of analytes during biotransformation processes and to provide information about utilizable sugars in fermentation media or in biological oxygen demand value determination. The final part of the review describes potential application of G. oxydans cells in the generation of electricity from complex fuels within microbial fuel cells by advanced direct electron transfer route between bacterial cells and electrodes.

Development of a Bioanode for Microbial Fuel Cells Based on the Combination of a MWCNT‐Au‐Pt Hybrid Nanomaterial, an Osmium Redox Polymer and Gluconobacter oxydans DSM 2343 Cells

“…The advantage of using G. oxydans cells is the adaptability to various substrates like glucose and glycerol ,,. Previous reports claimed that G. oxydans cells showed an improved electrochemical response to ethanol when fed with glycerol instead of glucose , . G. oxydans is often isolated from its natural (saccharide‐rich) medium prior to the application process, because these cells are able to use less than 10% of the D‐glucose present in the growth medium and this low consumption results in low bioconversion yields of the substrate .…”

“…In a previous study, a significant electrocatalytic effect of MWCNT‐Au‐Pt hybrid nanostructure has been demonstrated in comparison with non‐hybrid nanostructures which lead us to use this structure in our system . Because of the bioconversion capability of Gluconobacter oxydans DSM 2343 (G. oxydans) cells on a variety of organic compounds, these cells have been used for decades for both biosensor and MFC applications . It is possible to grow G. oxydans DSM 2343 cells specifically on a certain substrate like ethanol.…”

“…It is possible to grow G. oxydans DSM 2343 cells specifically on a certain substrate like ethanol. During the growth process, it has been reported that G. oxydans DSM 2343 cells show better electrochemical response to ethanol when they are fed with glycerol instead of glucose ,…”

“…[17] Because of the bioconversion capability of Gluconobacter oxydans DSM 2343 (G. oxydans) cells on a variety of organic compounds, these cells have been used for decades for both biosensor and MFC applications. [18][19][20][21][22][23][24] It is possible to grow G. oxydans DSM 2343 cells specifically on a certain substrate like ethanol. During the growth process, it has been reported that G. oxydans DSM 2343 cells show better electrochemical response to ethanol when they are fed with glycerol instead of glucose.…”

Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cellGluconobacter oxydansbiocatalyst immobilized in polyelectrolyte complex capsules

“…Membrane-bound dehydrogenases identified in Gluconobacter oxydans do not require the presence of cofactors which makes these cells highly efficient whole-cell biocatalysts for many biotechnological applications (Deppenmeier & ehrenreich 2009). the high biotechnological potential of G. oxydans for production of valuable chemicals was demonstrated recently by genome-scale reconstruction of the cell network (Wu et al 2014) and, moreover, application of cells is attractive also for other applications including construction of novel biosensors (schenkmayerová et al 2015) and biofuel cells (Bertokova et al 2015).…”