Gluconic acid: An antifungal agent produced by Pseudomonas species in biological control of take-all

Kaur, Ravneet; MacLeod, John K.; Foley, William J.; Nayudu, M. V.

doi:10.1016/j.phytochem.2005.12.011

Cited by 97 publications

(57 citation statements)

References 45 publications

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“…The direct role of gluconic acid production in the biocontrol activity of CHA0 remains unclear at present. Gluconic acid itself has been proposed to be an antifungal agent and to be involved in the biocontrol ability of an Australian nonfluorescent, non-DAPG-producing pseudomonad (30). A transposon insertion mutant of strain AN5 which had lost its ability to produce gluconic acid was partially impaired in its ability to inhibit G. graminis var.…”

Section: Discussionmentioning

confidence: 99%

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Role of Gluconic Acid Production in the Regulation of Biocontrol Traits of Pseudomonas fluorescens CHA0

Werra

Péchy-Tarr

Keel

et al. 2009

Appl Environ Microbiol

189

131

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The rhizobacterium Pseudomonas fluorescens CHA0 promotes the growth of various crop plants and protects them against root diseases caused by pathogenic fungi. The main mechanism of disease suppression by this strain is the production of the antifungal compounds 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin (PLT). Direct plant growth promotion can be achieved through solubilization of inorganic phosphates by the production of organic acids, mainly gluconic acid, which is one of the principal acids produced by Pseudomonas spp. The aim of this study was to elucidate the role of gluconic acid production in CHA0. Therefore, mutants were created with deletions in the genes encoding glucose dehydrogenase (gcd) and gluconate dehydrogenase (gad), required for the conversion of glucose to gluconic acid and gluconic acid to 2-ketogluconate, respectively. These enzymes should be of predominant importance for rhizosphere-colonizing biocontrol bacteria, as major carbon sources provided by plant root exudates are made up of glucose. Our results show that the ability of strain CHA0 to acidify its environment and to solubilize mineral phosphate is strongly dependent on its ability to produce gluconic acid. Moreover, we provide evidence that the formation of gluconic acid by CHA0 completely inhibits the production of PLT and partially inhibits that of DAPG. In the ⌬gcd mutant, which does not produce gluconic acid, the enhanced production of antifungal compounds was associated with improved biocontrol activity against take-all disease of wheat, caused by Gaeumannomyces graminis var. tritici. This study provides new evidence for a close association of gluconic acid metabolism with antifungal compound production and biocontrol activity in P. fluorescens CHA0.

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Section: Discussionmentioning

confidence: 99%

“…Indeed, gluconic acid metabolism has already been linked to antifungal activity. Recently, Kaur et al (30) proposed that gluconic acid produced by a nonfluorescent Pseudomonas isolate may be important for the biological control of take-all disease.…”

mentioning

confidence: 99%

Role of Gluconic Acid Production in the Regulation of Biocontrol Traits of Pseudomonas fluorescens CHA0

Werra

Péchy-Tarr

Keel

et al. 2009

Appl Environ Microbiol

189

131

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“…The TLC-based bio-autographs were developed as described by Kaur et al (2006). The YME agar supplemented with 10 4 spores/mL of the respective test fungus was poured on TLC plates with resolved APB.…”

Section: Tlc Agar Overlay Assaymentioning

confidence: 99%

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Kaur

Saini

2015

Span. j. agric. res.

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The cash crops grown in an agro-climatic region are prone to infection by various fungal pathogens. The use of chemical fungicides over the years has resulted in emergence of resistant fungal strains, thereby necessitating the development of effective and environmental friendly alternatives. The natural antagonistic interactions among different microbial populations have been exploited as an eco-friendly approach for controlling fungal pathogens resistant to synthetic chemicals. Morphologically distinct bacterial cultures (150), isolated from rhizospheric soils of wheat, rice, onion and tomato plants were screened for their antifungal potential against seven phytopathogenic fungi prevalent in the State of Punjab (India). The bacterial isolate R2, identified as Bacillus vallismortis, supported more than 50% inhibition of different phytopathogenic fungi (Alternaria alternata, Rhizoctonia oryzae, Fusarium oxysporum, Fusarium moniliforme, Colletotrichum sp, Helminthosporium sp and Magnaporthe grisea) in dual culture plate assay. The thin layer chromatography based bio-autography of acid-precipitated biomolecules (APB) indicated the presence of more than one type of antifungal molecule, as evidenced from zones of inhibition against the respective fungal pathogen. The initial analytical studies indicated the presence of surfactin, iturin A and fengycin-like compounds in APB. The antifungal activity of whole cells and APB of isolate R2 was evaluated by light and scanning electron microscopy. The wheat grains treated with APB and exposed to spores of A. alternata showed resistance to the development of black point disease, thereby indicating the potential application of R2 and its biomolecules at field scale level.

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“…Antibiotics are low weight molecular compound that suppress the development of plants pathogenic microorganisms. Phloroglucinols (Phl), 2-hexyl-5-propyl resorcinol (HPR), D-gluconic acid, hydrogen cyanide (HCN) and 2-hydroxymethylchroman-4-one have successfully been utilized as biocontrol agent (Perneel et al 2008;Kang et al 2004;Kaur et al 2006;Cazorla et al 2006).Elsewhere, increase productivity as a result of biocontrol inoculant was reported in S. rochei inhibition of pepper root rot caused by phytopthora (Ezziyyani et al, 2007); S. platensis against R. solani leaf blight/seedling blight of rice (Wan et al, 2008); Fusarium root rot and tomato wilt caused by S. griseoviridis (Minuto et al, 2006); S. hygroscopicus infection ofColletotrichum gloeosprioides anthacnose and in wide range of crops (Prapagdee et al, 2008) …”

Section: Antibiotics Productionmentioning

confidence: 99%

Plant Growth Promoting Rhizobacteria (PGPR): A Bioprotectant bioinoculant for Sustainable Agrobiology. A Review

Odoh¹

2017

Int. J. Adv. Res. Biol. Sci

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Plant growth promoting rhizobacteria (PGPR) involves the utilization of large array of soil bacteria to improve yield and plant growth. As free living and symbiotic rhizobacteria, PGPR colonizes extracellular and/or intracellular rhizoenvironment in search for carbon source while indirectly aiding plant growth. In the past few decades, focus has been on developing a biosafety agro base approach void of continuous burden on soil micro flora as a result of agrochemical application. However, with clear understanding of PGPR mechanisms of action; (i) biofertilization (ii) biostimulation (iii) and biocontrol, it create more hope on the possibility of curbing food insecurity, clean environmental sustenance and lower public health risk. Seeds or soil application of PGPRs inoculant enhances directly phosphates solubilization, atmospheric nitrogen fixation and secretion of plant hormones (indole acetic acid, gibberellins, cytokinins and ethylene) needed for growth and adaptation in stressed environment. As soil pathogen consistently rival the roles of these organisms, they (PGPRs)have developed over time wide spectrum of strategies in the form of systemic resistance, iron, space and nutrient competition, antibiotics synthesis, lytic acid production and hydrogen cyanide for efficient food productivity. In view of this, the review widen our scope on the use of PGPR as bioinoculant in sustainable agro practice and to serve as a wakeup call for it reception and implementation in the tropics where paucity of data on it use has long prevailed.

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Gluconic acid: An antifungal agent produced by Pseudomonas species in biological control of take-all

Cited by 97 publications

References 45 publications

Role of Gluconic Acid Production in the Regulation of Biocontrol Traits of Pseudomonas fluorescens CHA0

Role of Gluconic Acid Production in the Regulation of Biocontrol Traits of Pseudomonas fluorescens CHA0

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Plant Growth Promoting Rhizobacteria (PGPR): A Bioprotectant bioinoculant for Sustainable Agrobiology. A Review

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