Gluconacin from<i>Gluconacetobacter diazotrophicus</i>PAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria

Oliveira, Mona N.; Ramos, Edward; Drechsel, Marcela Motta; Vidal, Márcia Soares; Schwab, Stefan; Baldani, José Ivo

doi:10.1111/jam.14074

Cited by 16 publications

(13 citation statements)

References 47 publications

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“…Various antimicrobial compounds secreted by bacterial strains can exert bene cial effects in sustaining plant health. For example, the role of bacteriocin produced by endophytic bacteria as a source of new antibacterial compounds have synergistically been employed to upsurge antibiotic resistance (Lopes et al 2017;Oliveira et al 2018). Organic compounds synthesized by bacteria naturally play a vital role in the adaptation and survival of the host plant.…”

Section: Discussionmentioning

confidence: 99%

Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant

Adeleke

Babalola

2021

Curr Genet

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Plant growth-promotion screening and genome analysis of Stenotrophomonas indicatrix BOVIS40 were presented in this study. The genomic information reveals various genes underlining plant growth promotion and resistance to environmental stressors. The genome of S. indicatrix BOVIS40 harbors genes involved in the degradation and biotransformation of organic molecules. Also, other genes involved in bio lm production, chemotaxis, and agellation that facilitate bacterial colonization in the root endosphere and phytohormone genes that modulate root development and stress response in plants were detected in strain BOVIS40. IAA activity of the bacterial strain may be a factor responsible for root formation. Nevertheless, the results highlighted here provide insights into the genomic functions of S. indicatrix and which can be explored in agricultural management. Hence, a measurable approach to the S. indicatrix lifestyle can strategically provide several opportunities in their use as bioinoculants in developing environmentally friendly agriculture sustainably.

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Section: Discussionmentioning

confidence: 99%

Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant

Adeleke

Babalola

2021

Curr Genet

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“…The ICMP196 strain of X. albilineans, used as a model microorganism for the evaluation of bacteriocin activity, was obtained from the Collection of Phytopathogenic Bacteria of the Biological Institute of São Paulo. Culture media and growth conditions as well as the protocol used for recombinant Gluconacin expression have been described in our previous studies (Oliveira et al, 2018). Bacterial growth and recombinant Gluconacin expression were evaluated under the following incubation temperatures: 16, 25, 30 and 37 C. After the time of induction of protein expression (4 h), 3 ml of bacterial cultures were centrifuged (10,000g for 15 min), the supernatant discarded and the pellet resuspended in 300 μl of lysis buffer (50 mmol L À1 KH 2 PO 4 and K 2 HPO 4 , 400 mmol L À1 NaCl, 100 mmol L À1 KCl, 10% glycerol, 0.5% Triton X-100, pH 7.4).…”

Section: Microorganisms Growth Conditions and Gluconacin Expression In E Coli Bl21-aimentioning

confidence: 99%

“…Our team have cloned and demonstrated the heterologous expression of the GDI0415 gene, coding for Gluconacin, a bacteriocin produced by diazotrophic G. diazotrophicus PAL5. It has previously been demonstrated that this proteinaceous molecule has wide inhibitory activity against Gramnegative and Gram-positive bacteria, including the main causative agents of diseases of economic importance for large crops, such as sugarcane (Oliveira et al, 2018). However, this antimicrobial peptide has neither been characterised nor its mode of action elucidated, despite the fact that several bacteriocin-like compounds have been detected in G. diazotrophicus PAL5 (Blanco, Blanch, Piñ on, Legaz, & Vicente, 2005;Muñoz-Rojas, Fuentes-Ramirez, & Caballero-Mellado, 2005;Piñ on et al, 2002).…”

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Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

Ramos

Meneses

Vidal

et al. 2021

Annals of Applied Biology

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Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 αhelices and 11 β-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 μg μl À1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials.Scanning electron microscopy images showed that Gluconacin-treated X. albilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis.Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.

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“…Specific PCR primers were designed for the wzc gene (GDI2549) from G. diazotrophicus strain Pal5 and the MGD and MWzc mutants (Table 1). The E. coli expression system with Gateway ® technology (Thermo Fisher Scientific, Waltham, MA, USA) was applied to express the Wzc heterologous protein in E. coli strain BL21-AI™ using the suitable plasmids [34,35] and quantified [36]. The functionality of Wzc in different strains from G. diazotrophicus was tested by using heterologous expression in E. coli strain BL21-AI™.…”

Section: Cloning and Expression Of The Wzc Gene In E Coli Strain Bl21-aimentioning

confidence: 99%

Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in Gluconacetobacter diazotrophicus Strain Pal5

Wanderley

Sousa

Silva

et al. 2021

Life

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The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in Gluconacetobacter diazotrophicus. The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. G. diazotrophicus relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues. Here, we report that EPS from G. diazotrophicus strain Pal5 is a signal polymer that controls its own biosynthesis. EPS production depends on a bacterial tyrosine (BY) kinase (Wzc) that consists of a component that is able to phosphorylate a glycosyltranferase or to self-phosphorylate. EPS interacts with the extracellular domain of Wzc, which regulates kinase activity. In G. diazotrophicus strains that are deficient in EPS production, the Wzc is rendered inoperative by self-phosphorylation. The presence of EPS promotes the phosphorylation of a glycosyltransferase in the pathway, thus producing EPS. Wzc-mediated self-regulation is an attribute for the control of exopolysaccharide biosynthesis in G. diazotrophicus.

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Gluconacin fromGluconacetobacter diazotrophicusPAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria

Abstract: The production of a recombinant protein, named Gluconacin, opens new avenues for the agro-biotechnology application in agriculture, mainly with regard to the sugarcane crop.

Cited by 16 publications

References 47 publications

Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant

Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant

Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in Gluconacetobacter diazotrophicus Strain Pal5

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