Glucocorticoids suppress goblet cell metaplasia in natural and experimental asthma*1

Kim, E.Y.; Kim, J.; Morton, J. D.; Swanson, Suzanne; Patel, Anand C.; Castro, Mario; Holtzman, M.J.

doi:10.1016/j.jaci.2003.12.407

Cited by 2 publications

(1 citation statement)

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“…Relative expression of Muc5ac, Muc5b, and SCGB1A1 was reduced in the absence of HC. Previous studies have reported suppression of goblet cell abundance in the presence of glucocorticoids (40,58). Although not necessarily indicative of surface cell-type abundance, our data suggest increased transcription of mucin proteins in the presence of HC.…”

Section: Discussioncontrasting

confidence: 58%

Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance

Zaidman

Panoskaltsis‐Mortari

O’Grady

2016

American Journal of Physiology-Cell Physiology

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Glucocorticoids strongly influence the mucosal-defense functions performed by the bronchial epithelium, and inhaled corticosteroids are critical in the treatment of patients with inflammatory airway diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis. A common pathology associated with these diseases is reduced mucociliary clearance, a defense mechanism involving the coordinated transport of salt, water, and mucus by the bronchial epithelium, ultimately leading to retention of pathogens and particles in the airways and to further disease progression. In the present study we investigated the role of hydrocortisone (HC) in differentiation and development of the ion transport phenotype of normal human bronchial epithelial cells under air-liquid interface conditions. Normal human bronchial epithelial cells differentiated in the absence of HC (HC0) showed significantly less benzamil-sensitive short-circuit current than controls, as well as a reduced response after stimulation with the selective β2-adrenergic receptor agonist salbutamol. Apical membrane localization of epithelial Na(+) channel α-subunits was similarly reduced in HC0 cells compared with controls, supporting a role of HC in the trafficking and density of Na(+) channels in the plasma membrane. Additionally, glucocorticoid exposure during differentiation regulated the transcription of cystic fibrosis transmembrane conductance regulator and β2-adrenergic receptor mRNAs and appeared to be necessary for the expression of cystic fibrosis transmembrane conductance regulator-dependent anion secretion in response to β2-agonists. HC had no significant effect on surface cell differentiation but did modulate the expression of mucin mRNAs. These findings indicate that glucocorticoids support mucosal defense by regulating critical transport pathways essential for effective mucociliary clearance.

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Section: Discussioncontrasting

confidence: 58%

Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance

Zaidman

Panoskaltsis‐Mortari

O’Grady

2016

American Journal of Physiology-Cell Physiology

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“Hit-and-Run” Effects of Paramyxoviruses as a Basis for Chronic Respiratory Disease

Holtzman¹,

Shornick²,

Grayson³

et al. 2004

Pediatric Infectious Disease Journal

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Our studies show that antiviral defense depends on a special network of epithelial immune response genes that signal to the adaptive immune system. Viruses ordinarily trigger this network, but it is also permanently activated in asthma, even in the absence of viral infection. In addition, we find that, in susceptible genetic backgrounds, respiratory viruses cause a "hit-and-run" phenomenon indicated by the development of an asthmatic phenotype long after the infection has cleared. On the basis of this information, we developed a new scheme for asthma pathogenesis that includes epithelial, viral and allergic components and allows viral reprogramming of host behavior.

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Glucocorticoids suppress goblet cell metaplasia in natural and experimental asthma*1

Cited by 2 publications

References 0 publications

Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance

Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance

“Hit-and-Run” Effects of Paramyxoviruses as a Basis for Chronic Respiratory Disease

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