Glucocorticoid receptor expression in whole blood with preterm infants

Go, Hayato; Sato, Maki; Hashimoto, Koichi; Imamura, Takashi; Kaneko, M; Sato, Toshiko; Goto, Aya; Ishi‐i, Tsutomu; Ishibashi, Naohisa; Momoi, Nobuo; Fujimori, Keiya; Hosoya, Mitsuaki

doi:10.1515/jpem-2012-0170

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several polymorphisms in the glucocorticoid receptor gene (NR3C1) are linked to altered function of glucocorticoid receptor. SNPs may increase glucocorticoid sensitivity in vivo , and have been associated with birth weight in preterm neonates [62] , [63] .…”

Section: Discussionmentioning

confidence: 99%

Pathway Analysis of Genetic Factors Associated with Spontaneous Preterm Birth and Pre-Labor Preterm Rupture of Membranes

et al. 2014

View full text Add to dashboard Cite

BackgroundPre-term birth (PTB) remains the leading cause of infant mortality and morbidity. Its etiology is multifactorial, with a strong genetic component. Genetic predisposition for the two subtypes, spontaneous PTB with intact membranes (sPTB) and preterm prelabor rapture of membranes (PPROM), and differences between them, have not yet been systematically summarised.Methods and findingsOur literature search identified 15 association studies conducted in 3,600 women on 2175 SNPs in 274 genes. We used Ingenuity software to impute gene pathways and networks related to sPTB and PPROM. Detailed insight in the defined functional ontologies clearly separated integrated datasets for sPTB and PPROM. Our analysis of upstream regulators of genes suggests that glucocorticoid receptor (NR3C1), peroxisome proliferator activated receptor γ (PPARG) and interferon regulating factor 3 (IRF3) may be sPTB specific. PPROM-specific genes may be regulated by estrogen receptor2 (ESR2) and signal transducer and activator of transcription (STAT1). The inflammatory transcription factor NFκB is linked to both sPTB and PPROM, however, their inflammatory response is distinctly different.ConclusionsBased on our analyses, we propose an autoimmune/hormonal regulation axis for sPTB, whilst pathways implicated in the etiology of PPROM include hematologic/coagulation function disorder, collagen metabolism, matrix degradation and local inflammation. Our hypothesis generating study has identified new candidate genes in the pathogenesis of PPROM and sPTB, which should be validated in large cohorts.

show abstract

Section: Discussionmentioning

confidence: 99%

Pathway Analysis of Genetic Factors Associated with Spontaneous Preterm Birth and Pre-Labor Preterm Rupture of Membranes

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) was performed using the TaqMan method. The sequences of the primers and probes used for GRα were designed to span exons 8 and 9α, the specific sequence for GRα, as previously described [12, 22]: GRα-F 5′-TTGGATTCTATGCATGAAGTGGTT-3′, GRα-R 5′-CAATACTCATGGTCTTATCCAAAAATG-3′, and GRα-probe FAM-TCTCCTTAACTATTGCTTCCA-TAMRA. The primers and probes for GRβ and actin beta (ACTB) were purchased from ABI (Applied Biosystems, Foster city, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies reported a change in the GC receptor (GR) expression pattern, which is essential for regulating the transcription of GC-responsive genes in the perinatal period [12]. GC activity is mediated by GR, an intracellular receptor protein, which belongs to the nuclear receptor superfamily and regulates the expression of GC-responsive genes [13, 14].…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid Receptor Expression Pattern in Very Low Birth Weight Infants Changes Drastically within the First Week of Life

et al. 2022

View full text Add to dashboard Cite

Introduction We herein examined changes in expression levels of the glucocorticoid receptor subtypes GRα and GRβ in very low-birth weight (VLBW) and term infants to clarify time-dependent changes in glucocorticoid sensitivity after birth. Methods Whole blood samples were collected at birth and on postnatal days 4 to 7, and the mRNA expression levels of GRα and GRβ were measured using RT-qPCR. The relative gene expression levels of GRα and GRβ as the target genes normalized to actin beta as the endogenous control were calculated by the comparative cycle threshold method. Results The GRα/GRβ expression ratio at birth was significantly lower in 32 VLBW cesarean section (CS) infants than in term planned CS infants (median (IQR), 1.5 (1.1-1.8)- and 1.1 (0.7-1.6)-fold change, p <0.05). Furthermore, the GRα/GRβ expression ratio increased from day 0 to days 4 to 7 (1.0 (0.6-1.4)- and 1.7 (0.6-1.4)-fold change, p <0.01) in 43 VLBW infants. Discussion / Conclusion The present results suggests that glucocorticoid sensitivity in VLBW infants increases after birth and this rapid change may play a role in surviving critical postnatal events.

show abstract

Glucocorticoid receptor expression in whole blood with preterm infants

Abstract: Importantly, in preterm infants, both the expression of GRβ and the GRα/GRβ ratio were associated with birth weight. Further studies with larger populations are necessary to determine the relation between the expression of GR and the clinical relevance of preterm infants.

Cited by 2 publications

References 0 publications

Pathway Analysis of Genetic Factors Associated with Spontaneous Preterm Birth and Pre-Labor Preterm Rupture of Membranes

Pathway Analysis of Genetic Factors Associated with Spontaneous Preterm Birth and Pre-Labor Preterm Rupture of Membranes

Glucocorticoid Receptor Expression Pattern in Very Low Birth Weight Infants Changes Drastically within the First Week of Life

Contact Info

Product

Resources

About