Glucocorticoid metabolism in equine follicles and oocytes

Scarlet, D.; Ille, Natascha; Ertl, Reinhard; Alves, Bênner Geraldo; Gastal, G. D. A.; Paiva, S. O.; Gastal, M.O.; Aurich, Christine

doi:10.1016/j.domaniend.2016.10.004

Cited by 23 publications

(18 citation statements)

References 42 publications

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“…Although intrafollicular factors and steroids have been extensively studied during the process of deviation, knowledge is still lacking with respect to the role of 5-HT concerning dominance and pre-ovulatory phases of the oestrous cycle. Most studies on FF factors, as steroid hormones, in mares have categorized the follicles of mares during the period of follicular deviation (Donadeu & Ginther, 2002;Gastal, Gastal, Bergfelt, & Ginther, 1997;Gastal, Gastal, Wiltbank, & Ginther, 1999;Scarlet et al, 2017) and in puberty (Młodawska et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Intrafollicular and systemic serotonin, oestradiol and progesterone concentrations in cycling mares

Satué

Fazio

Ferlazzo

et al. 2019

Reprod Domestic Animals

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The hypothesis that a local serotonergic network might also exist in the follicle of mares remains poorly documented, with exception for humans and laboratory species. For this reason, the aim of the present study was to clarify this possibility, investigating intrafollicular serotonin concentrations of the cycling mare at ovulation time. Sixty ovaries collected from 30 clinically healthy mares of slaughterhouse meat production with clinically normal reproductive tracts after slaughtering were evaluated. Blood samples were taken prior to sacrifice. Follicles were classified in three categories in relation to size, as small (20–30 mm), medium (31–40 mm) and large (>41 mm), and the follicular fluid samples were extracted from each follicle. Intrafollicular and systemic serotonin (5‐HT), oestradiol‐17β (E2) and progesterone (P4) were determined by means of enzyme‐linked immunosorbent assay and RIA, respectively. Intrafollicular 5‐HT, E2 and P4 concentrations were higher than systemic ones (p < .05). 5‐HT concentrations increased in larger compared to medium follicles, without differences compared to small size follicles (p < .05). 5‐HT and E2 (r = .79) and 5‐HT and P4 (r = .79; p < .05) were positively correlated. 5‐HT and P4 concentrations in follicular fluid increased progressively with the increase in follicular size (p < .05). Follicle diameter and E2 (r = .85) and P4 (r = .68) were correlated (p < .05). Since serotonin interacts with steroids, its role on steroidogenesis during growth of the dominant follicle may be suggested.

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Section: Discussionmentioning

confidence: 99%

Intrafollicular and systemic serotonin, oestradiol and progesterone concentrations in cycling mares

Satué

Fazio

Ferlazzo

et al. 2019

Reprod Domestic Animals

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“…However, in the presence of 18β-glycyrrhetinic acid (GA), a specific inhibitor of HSD11B2, lower levels of cortisol suppressed the oocyte maturation in mice and pigs (32). On the other hand, high levels of cortisol did not affect oocyte maturation in horses (35). These authors demonstrated that equine COC expresses HSD11B2, but not HSD11B1.…”

Section: Discussionmentioning

confidence: 79%

Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF

Tetsuka

Tanakadate

2019

Endocrine Connections

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The bovine cumulus-oocyte complex (COC) is capable of converting cortisone, an inert glucocorticoid to active cortisol. This mechanism is mediated by 11β-hydroxysteroid oxidoreductase type 1 (HSD11B1), whose expression dramatically increases in the mature COC. In this study, we investigate the time course expression of HSD11B1 and the enzyme activity in the bovine COC undergoing maturation and fertilization in relation to key events taking place in the COC. Bovine COCs were subjected to in vitro maturation (IVM) and fertilization (IVF). The activities of HSD11B1 and HSD11B2, which mediates the opposite reaction, were measured using a radiometric conversion assay. In parallel studies, cumulus expansion, P4 production and the expression of genes associated with ovulation were measured. The reductive activity of HSD11B1 increased in the latter half of IVM and remained high during IVF, whereas the oxidative activity of HSD11B2 remained unchanged over both periods. Consequently, the net glucocorticoid metabolism in the bovine COC shifted from inactivation to activation around the time of ovulation and fertilization. The increase in HSD11B1 expression lagged behind that of P4 increase and cumulus expansion but ahead of the expressions of genes responsible for PGE2 synthesis. The reductive activity of HSD11B1 was well correlated with the cumulus expansion rate. This outcome indicates that the ability of the cumulus to activate glucocorticoids is related to its ability to synthesize hyaluronan. These results also indicate that the activation of HSD11B1 is an integral part of the sequential events taking place at the ovulation and fertilization in the bovine COC.

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“…Interestingly, in pigs, high 11β-HSD1-mediated cortisol production activity has been related to the inhibition of porcine oocyte maturation [41][42][43] in contrast to species such as the cow, where it appears to be promoted after ovulation [13,44,45] and is also beneficial for oocyte maturation and fertilization, at least in vitro [44,46,47]. Furthermore, in equine species, cortisol does not affect oocyte maturation in vitro [48]. The direct effect of GCs in mammalian species appears to vary greatly, implying that GC-sensitivity and regulation might be species-specific, and in part driven by diverse ratios of HSD11B2/1 [41].…”

Section: Discussionmentioning

confidence: 99%

Natural Mating Differentially Triggers Expression of Glucocorticoid Receptor (NR3C1)-Related Genes in the Preovulatory Porcine Female Reproductive Tract

Ruiz-Conca

Gardela

Martínez

et al. 2020

IJMS

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Mating initiates dynamic modifications of gene transcription in the female reproductive tract, preparing the female for fertilization and pregnancy. Glucocorticoid signaling is essential for the homeostasis of mammalian physiological functions. This complex glucocorticoid regulation is mediated through the glucocorticoid receptor, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1/GR) and related genes, like 11β-hydroxysteroid dehydrogenases (HSD11Bs) and the FK506-binding immunophilins, FKBP5 and FKBP4. This study tested the transcriptome changes in NR3C1/GR regulation in response to natural mating and/or cervical deposition of the sperm-peak ejaculate fraction collected using the gloved-hand method (semen or only its seminal plasma), in the preovulatory pig reproductive tract (cervix to infundibulum, 24 h after mating/insemination/infusion treatments). Porcine cDNA microarrays revealed 22 NR3C1-related transcripts, and changes in gene expression were triggered by all treatments, with natural mating showing the largest differences, including NR3C1, FKBP5, FKBP4, hydroxysteroid 11-beta dehydrogenase 1 and 2 (HSD11B1, HSD11B2), and the signal transducer and activator of transcription 5A (STAT5A). Our data suggest that natural mating induces expression changes that might promote a reduction of the cortisol action in the oviductal sperm reservoir. Together with the STAT-mediated downregulation of cytokine immune actions, this reduction may prevent harmful effects by promoting tolerance towards the spermatozoa stored in the oviduct and perhaps elicit spermatozoa activation and detachment after ovulation.

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Glucocorticoid metabolism in equine follicles and oocytes

Cited by 23 publications

References 42 publications

Intrafollicular and systemic serotonin, oestradiol and progesterone concentrations in cycling mares

Intrafollicular and systemic serotonin, oestradiol and progesterone concentrations in cycling mares

Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF

Natural Mating Differentially Triggers Expression of Glucocorticoid Receptor (NR3C1)-Related Genes in the Preovulatory Porcine Female Reproductive Tract

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