Glucocorticoid‐inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells

Abstract

Inducible, vector-based, expression systems that allow fine control of transgene expression are gaining more and more use in fundamental research as well as in therapeutic applications. In an effort to develop a tightly regulated heterologous expression system for Drosophila Schneider 2 cells, three different inducible reporter constructs were compared. These comprised six copies of the glucocorticoid response element fused to one of three distinct types of Drosophila gene promoters: (1) a TATA-box containing, (2) a TATA-less and (3) a bidirectional core sequence. These were fused to a luciferase reporter gene. The promoter constructs displayed different basal as well as agonist-induced activities. The implications of the observations made are discussed in the context of promoter properties and of induction of genes that may be studied in Drosophila.

The Cellular Chaperone Heat Shock Protein 90 Facilitates Flock House Virus RNA Replication in Drosophila Cells

“…4C). As a positive control, we used a luciferase reporter gene under the control of an Hsp90-dependent glucocorticoid receptor-responsive promoter (37) and found that 5 M geldanamycin reduced dexamethasone-induced luciferase activity by 90% (Fig. 4C, left bars).…”