Glucocorticoid and Sex Hormones as Activating or Modulating Factors for Expression of Cyp2b-9 and Cyp2b-L0 in the Mouse Liver and Hepatocytes

Nemoto, Nobuo; Sakurai, Junko

doi:10.1006/abbi.1995.1294

Cited by 58 publications

(50 citation statements)

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“…The liver was perfused with collagenase, and viable hepatocytes were isolated by Percoll isodensity centrifugation as described. 17) Standard culture conditions were as follows: the cells were dispersed in Waymouth MB752/1 medium containing bovine serum albumin (2 g/l), insulin (0.5 mg/l), transferrin (0.5 mg/l), and selenium (0.5 mg/l), and seeded at a density of 5ϫ10 5 cells/1.5 ml/35-mm collagen-coated dish. The Waymouth medium did not contain phenol red, a pH indicator, to exclude the possibility of estrogen-like action.…”

Section: Methodsmentioning

confidence: 99%

Potent Modification of Inducible CYP1A1 Expression by Flavonoids

Chatuphonprasert

Kondo

Jarukamjorn

et al. 2010

Biological & Pharmaceutical Bulletin

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The present study examined modifications of b-naphthoflavone (b-NF)-induced cytochrome P450 1A1 (CYP1A1) expression by flavonoids in mouse hepatocytes in primary culture. Some flavonoids (apigenin, chrysin, flavone, flavanone, galangin, luteolin, and naringenin) by themselves induced CYP1A1 mRNA expression, especially flavone which was even more effective than b-NF. The effect on b-NF-induced CYP1A1 mRNA expression was varied, namely additive, suppressive, or both. An additive effect was observed after combined treatment with flavanone, naringenin, and chrysin, whereas kaempferol, myricetin, and quercetin decreased CYP1A1 levels. Apigenin, chrysin, galangin, luteolin, and morin synergistically enhanced b-NF-induced CYP1A1 expression at 24 h, but considerably suppressed it at 9 h. The structure-activity relationship of flavonoids affecting CYP1A1 expression as inducers or inhibitors is discussed. The present observations suggest the need to reveal the mechanism by which CYP1A1 expression is modified by flavonoids for risk assessment, since CYP1A1 activates environmental carcinogenic polycyclic hydrocarbons and flavonoids are major constituents in food.

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Section: Methodsmentioning

confidence: 99%

Potent Modification of Inducible CYP1A1 Expression by Flavonoids

Chatuphonprasert

Kondo

Jarukamjorn

et al. 2010

Biological & Pharmaceutical Bulletin

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“…Hepatic total RNA was prepared from hepatocytes as described previously (Nemoto and Sakurai, 1995). Semiquantitative RT-PCR of CYP3A44 and GAPDH with 32 P-radiolabeled primers was performed using a TaKaRa RNA PCR Kit (AMV) version 3.0 as described previously (Sakuma et al, 2000(Sakuma et al, , 2002.…”

Section: Methodsmentioning

confidence: 99%

“…Eight-week-old female ddY mice were purchased from Japan SLC, Inc. (Shizuoka, Japan). The livers were perfused with collagenase-containing Hanks' solution, and viable hepatocytes were isolated by Percoll isodensity centrifugation as described previously (Nemoto and Sakurai, 1995) and seeded in dishes at a density of 2 ϫ 10 6 cells/4 ml/60 mm. The Waymouth medium did not contain phenol red, a pH indicator, to exclude estrogen-like action.…”

Section: Methodsmentioning

confidence: 99%

Involvement of Glucocorticoid Receptor and Pregnane X Receptor in the Regulation of Mouse CYP3A44 Female-Predominant Expression by Glucocorticoid Hormone

Bhadhprasit¹,

Sakuma²,

Hatakeyama³

et al. 2007

Drug Metab Dispos

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“…For example, it was demonstrated that estradiol-one of the steroid hormones secreted during pregnancy by the placenta-induces CYP2B6 mRNA activity and expression in human hepatocytes (Choi et al, 2013;Dickmann and Isoherranen, 2013). Data obtained from rodent studies also demonstrated that estradiol can induce the expression of cytochrome P450 enzymes (Nemoto and Sakurai, 1995). Furthermore, the increased clearance (CL) during pregnancy as compared with the postpartum period of efavirenz and methadone (Pond et al, 1985;Wolff et al, 2005;Cressey et al, 2012), two drugs that are metabolized mainly by hepatic CYP2B6, also suggests increased activity of the enzyme.…”

Section: Introductionmentioning

confidence: 88%

Metabolism and Disposition of Bupropion in Pregnant Baboons (Papio cynocephalus)

et al. 2014

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Recent in vitro data obtained in our laboratory revealed similarities between baboons and humans in the biotransformation of bupropion (BUP) by both hepatic and placental microsomes. These data supported the use of baboons to study BUP biotransformation during pregnancy. The aim of this investigation was to determine the pharmacokinetics of BUP in baboons during pregnancy and postpartum, as well as fetal exposure to the drug after intravenous administration. Pregnant baboons (n = 5) received a single intravenous bolus dose of bupropion hydrochloride (1 mg/kg) at gestational ages 94-108 days (midpregnancy), 142-156 days (late pregnancy), and 6 weeks postpartum. Blood and urine samples were collected for 12 and 24 hours, respectively. The concentrations of BUP, hydroxybupropion (OH-BUP), threohydrobupropion, and erythrohydrobupropion in plasma were determined by liquid chromatography-tandem mass spectrometry. Relative to the postpartum period, the average midpregnancy clearance of BUP trended higher (3.6 6 0.15 versus 2.7 6 0.28 l/h per kg) and the average C max (294 6 91 versus 361 6 64 ng/ml) and the area under the curve (AUC) of BUP values (288 6 22 versus 382 6 42 h·ng/ml) trended lower. AUC OH-BUP also tended to be lower midpregnancy compared with postpartum (194 6 76 versus 353 6 165 h·ng/ml). Whereas the observed trend toward increased clearance of BUP during baboon pregnancy could be associated with a pregnancy-induced increase in its biotransformation, the trend toward increased renal elimination of OH-BUP may overshadow any corresponding change in the hydroxylation activity of CYP2B.

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Glucocorticoid and Sex Hormones as Activating or Modulating Factors for Expression of Cyp2b-9 and Cyp2b-L0 in the Mouse Liver and Hepatocytes

Cited by 58 publications

References 0 publications

Potent Modification of Inducible CYP1A1 Expression by Flavonoids

Potent Modification of Inducible CYP1A1 Expression by Flavonoids

Involvement of Glucocorticoid Receptor and Pregnane X Receptor in the Regulation of Mouse CYP3A44 Female-Predominant Expression by Glucocorticoid Hormone

Metabolism and Disposition of Bupropion in Pregnant Baboons (Papio cynocephalus)

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