Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis

Lee, Hsiang-Ping; Wang, Shih‐Wei; Wu, Yang‐Chang; Tsai, Chang‐Hai; Tsai, Fuu‐Jen; Chung, Jing‐Gung; Huang, Chih‐Yang; Yang, Jai Sing; Hsu, Yu-Ting; Yin, Mei‐chin; Li, Te‐Mao; Tang, Chih‐Hsin

doi:10.1080/09540105.2019.1660623

Cited by 75 publications

(57 citation statements)

References 50 publications

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“…Extracted proteins were resolved by SDS-PAGE and transferred to Immobilon ® PVDF membranes. Western blot analysis was performed according to our previous reports [22][23][24].…”

Section: Western Blot Analysismentioning

confidence: 99%

S1P promotes IL-6 expression in osteoblasts through the PI3K, MEK/ERK and NF-κB signaling pathways

Hu¹,

Huang²,

Tzeng³

et al. 2020

Int. J. Med. Sci.

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Rheumatoid arthritis (RA) is a systemic autoimmune inflammatory disease, in which the immune system attacks joint tissue. Interleukin (IL)-6 is a key proinflammatory cytokine in RA progression. Sphingosine-1-phosphate (S1P), a platelet-derived lysophospholipid mediator, reportedly regulates osteoimmunology. Here, we examined the effects of S1P on IL-6 expression in osteoblasts. Our results and records from the Gene Expression Omnibus (GEO) database demonstrate higher levels of IL-6 in patients with RA compared with those with osteoarthritis. Stimulation of osteoblasts with S1P increased mRNA and protein expression of IL-6. PI3K, MEK, ERK and NF-B inhibitors and their small interfering RNAs (siRNAs) reduced S1P-promoted IL-6 expression. S1P also facilitated PI3K, MEK/ERK and NF-B signaling cascades. Our results indicate that S1P promotes the expression of IL-6 in osteoblasts via the PI3K, MEK/ERK and NF-B signaling pathways.

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“…Extracted proteins were resolved by SDS-PAGE and transferred to Immobilon ® PVDF membranes. Western blot analysis was performed according to our previous reports [22][23][24].…”

Section: Western Blot Analysismentioning

confidence: 99%

S1P promotes IL-6 expression in osteoblasts through the PI3K, MEK/ERK and NF-κB signaling pathways

Hu¹,

Huang²,

Tzeng³

et al. 2020

Int. J. Med. Sci.

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“…The M-MLV RT kit (Invitrogen, Thermo Fisher Scientific; Waltham, MA, USA) and the Mir-X™ miRNA First-Strand Synthesis kit (Clontech; Mountain View, CA, USA) were used to perform reverse transcription of total RNA into complementary DNA (cDNA). PCR amplification was performed as previously described [32,38,39].…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qpcr)mentioning

confidence: 99%

WISP-1 Promotes Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells via the miR-153-3p/Snail Axis

Chang

Lien

Tsai

et al. 2019

Cancers

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Around half of all patients with oral squamous cell carcinoma (OSCC) present with lymphatic metastasis, a strong predictor of poor survival. Improving survival rates depends on preventing the first step in the “invasion-metastasis cascade,” epithelial-to-mesenchymal transition (EMT), and developing antilymphangiogenesis therapies that antagonize lymphatic metastasis. The extracellular matrix-related protein WISP-1 (WNT1-inducible signaling pathway protein-1) stimulates bone remodeling and tumor progression. We have previously reported that WISP-1 promotes OSCC cell migration and lymphangiogenesis induced by vascular endothelial growth factor C (VEGF-C). This investigation sought to determine the role of WISP-1 in regulating EMT in OSCC. Our analysis of oral cancer data from The Cancer Genome Atlas (TCGA) database revealed significant and positive associations between levels of WISP-1 expression and clinical disease stage, as well as regional lymph node metastasis. We also found higher levels of WISP-1 expression in serum samples obtained from patients with OSCC compared with samples from healthy controls. In a series of in vitro investigations, WISP-1 activated EMT signaling via the FAK/ILK/Akt and Snail signaling transduction pathways and downregulated miR-153-3p expression in OSCC cells. Our findings detail how WISP-1 promotes EMT via the miR-153-3p/Snail axis in OSCC cells.

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“…As with vascular endothelial growth factor (VEGF), the role of Angpt2 has been characterized as being pro-angiogenic or pro-inflammatory in cancer progression [21][22][23][24]. However, there are few reports regarding Angpt2 polymorphisms in various types of cancers.…”

Section: Discussionmentioning

confidence: 99%

Angiopoietin-2 gene polymorphisms are biomarkers for the development and progression of colorectal cancer in Han Chinese

Du¹,

Tang²,

Li³

et al. 2020

Int. J. Med. Sci.

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Colorectal cancer (CRC) is one of the most common cancers in Han Chinese and is characterized by low rates of early diagnosis and poor survival rates. Angiopoietin-2 (Ang2), an endothelial tyrosine kinase, is involved in CRC progression, but little is known about the association between single nucleotide polymorphisms (SNPs) and diagnosis or prognosis of CRC. This study reports on the association between 5 SNPs of the Angpt2 gene (rs2442598, rs734701, rs1823375, 11137037, and rs12674822) and CRC susceptibility as well as clinical outcomes in 379 patients with CRC and in 1,043 cancer-free healthy controls. Carriers of the CG allele at rs1823375 and those with the GT+TT allele of the variant rs12674822 were at greater risk of CRC than their respective wild-type counterparts. Moreover, carriers of the GT or GT+TT allele in rs12674822 were significantly more likely to have tumor involvement in both the colon and rectum compared with wild-type (GG) carriers, while 5-year progression-free survival was also significantly worse in those carrying the GT+TT allele in rs12674822 compared with wild-type carriers. Our study is the first to describe correlations between Angpt2 polymorphisms and CRC development and progression in people of Chinese Han ethnicity.

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Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis

Cited by 75 publications

References 50 publications

S1P promotes IL-6 expression in osteoblasts through the PI3K, MEK/ERK and NF-κB signaling pathways

S1P promotes IL-6 expression in osteoblasts through the PI3K, MEK/ERK and NF-κB signaling pathways

WISP-1 Promotes Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells via the miR-153-3p/Snail Axis

Angiopoietin-2 gene polymorphisms are biomarkers for the development and progression of colorectal cancer in Han Chinese

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