Glucoamylase ausEndomycopsis bispora I. Zur Produktion des Enzyms in Schüttelkultur

Effect of medium composition on amylase production by some starch-degrading yeasts

“…This indicates that only part of the amylase activity is constitutive and that higher amylase production can be induced by a-l,4-glucans (maltose, dextrin, soluble starch). This phenomenon is wellknown for several amylolytic enzymes of bacterial, fungal [19] and yeast origin [6,8,9,10,17]. C tsukubaensis showed one of the highest amounts of amylase activity in the presence of soluble starch.…”

“…The highest extracellular amylase activities in the presence of different nitrogen sources and soluble starch as a carbon source, are compiled in table 2. The initial pH of the medium was adjusted Table 1 Effect of the initial pH and of the type of carbon source on the production of extracellular amylases by several yeast species growing in a buffered yeast nitrogen-base medium CSL is often used in fermentation media [19] and it has also been successfully applied for amylase and biomass production by some amylolytic yeast species [6,20,21] and for enhanced synthesis of inulinase by Kluyveromyces fragilis [22]. CSL in the medium resulted in a lowered extracellular amylase activity with the Candida strains and Cr.…”

“…During the last decade, amylolytic yeasts have been intensively investigated as promising microorganisms for the conversion of starchy materials into single-cell protein or ethanol [1][2][3][4][5][6][7][8][9]. The production and secretion of amylases is highly dependent on the cultural conditions.…”

“…The production and secretion of amylases is highly dependent on the cultural conditions. The influence of pH, the type of carbon or nitrogen source, and of the presence of salts has been investigated for several amylolytic yeast species [6,[10][11][12][13][14].…”