Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment.

Nishino, Tatsuya; Kodaira, Tsukasa; Shin, Seok-Hwan; Imagawa, Kenichi; Shima, Kenji; Kumahara, Yuichi; Yanaihara, Chizuko; Yanaihara, N

doi:10.1093/clinchem/27.10.1690

Cited by 95 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glucagon immunoreactivity in culture media was measured using OAL 123; Daiichi Radioisotope Institute (Tokyo, Japan) [22], the antibody specific for pancreatic glucagon.…”

Section: Assay and Statistical Analysismentioning

confidence: 99%

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

et al. 1995

View full text Add to dashboard Cite

In pancreatic alpha cells, the existence and function of the insulin receptor has not yet been fully established. In this study, to confirm the expression of functional insulin receptors in pancreatic alpha cells, we performed: 1) insulin receptor binding assay, 2) Northern blot analysis and RT-PCR (reverse transcription-polymerase chain reaction) amplification of insulin receptor mRNA, 3) immunocytochemical staining, 4) biosynthetic labelling of insulin receptor protein using [35S]methionine, 5) analysis of insulin-stimulated autophosphorylation of the insulin receptor in glucagon secreting cell lines, In-R1-G9 and alpha TC clone 6 cells. Glucagon secretion decreased with the addition of insulin in both cells. The receptor binding studies using [125I-Tyr-A14] insulin revealed that both cells possessed a significant number of insulin receptors (In-R1-G9:K1 = 2.1 x 10(9) mol/l-1, K2 = 6.2 x 10(7) mol/l-1, R1 = 0.27 x 10(4), R2 = 1.86 x 10(4) sites/cell; alpha TC clone 6: K1 = 2.1 x 10(9) mol/l-1, K2 = 7.3 x 10(7) mol/l-1, R1 = 0.27 x 10(4), R2 = 1.95 x 10(4) sites/cell). Northern blot analysis as well as RT-PCR amplification showed the mRNA specific for insulin receptor in both cells. By immunocytochemical staining using anti-insulin receptor alpha-subunit antibody, positive immunostaining for insulin receptor was observed in both cells. [35S]Methionine labelling of both cells followed by immunoprecipitation using anti-insulin receptor antibody showed the correct size of the insulin receptor protein. The insulin receptor expressed in these cells underwent autophosphorylation by insulin stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…Glucagon immunoreactivity in culture media was measured using OAL 123; Daiichi Radioisotope Institute (Tokyo, Japan) [22], the antibody specific for pancreatic glucagon.…”

Section: Assay and Statistical Analysismentioning

confidence: 99%

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Immunoreactive insulin was measured with a commercially available kit (Eiken Chemical Co., Tokyo, Japan) based on a radioimmunoassay using rat insulin (Novo Research Institute, Bagsvaerd, Denmark) as a standard. Immunoreactive glueagon was measured by a previously described method [16] using antiserum to synthetic glucagon 19-29 [17].…”

Section: Methodsmentioning

confidence: 99%

Neurotransmitters partially restore glucose sensitivity of insulin and glucagon secretion from perfused streptozotocin-induced diabetic rat pancreas

et al. 1995

View full text Add to dashboard Cite

To elucidate the mechanisms of insensitivity of hormone secretion to glucose in streptozotocin-induced diabetic rat islets, we investigated the effects of acetylcholine (ACh) and norepinephrine on insulin and glucagon secretion in response to changes in glucose concentration, using perfused pancreas preparations. Basal insulin secretion at a blood glucose level of 5.6 mmol/l was significantly higher and basal glucagon secretion significantly lower in streptozotocin-induced diabetic rats than in controls, and neither high (16.7 mmol/l) nor low (1.4 mmol/l) blood glucose concentrations influenced insulin or glucagon secretion. Addition of 10(-6) mol/l ACh to the perfusate increased glucose-stimulated insulin secretion. Also, 10(-6) mol/l ACh, 10(-7) mol/l norepinephrine, as well as a combination of both, induced marked glucagon secretion, this was suppressed by high blood glucose level. Although simultaneous addition of 10(-6) mol/l ACh and 10(-7) mol/l norepinephrine induced only a slight increase in glucagon secretion in response to glucopenia, there was a significant increase in glucagon secretion in conjunction with an ambient decrease in insulin. Histopathological examination revealed a marked decline in acetylcholinesterase and monoamine-oxidase activities in the islets of streptozotocin-induced diabetic rats. We speculate that reduction of the potentiating effects of ACh and norepinephrine lessens glucose sensitivity of islet beta and alpha cells in this rat model of diabetes.

show abstract

“…Glucagon concentration was measured by the method of Nishino et al [19] using a Rat/Human Glucagon EIA. Insulin concentration was measured by the sandwich technique, which uses a monoclonal antibody (Rebis R insulin kit).…”

Section: Hormone Assaysmentioning

confidence: 99%

Changes of glucose metabolism and skin-collagen neogenesis in vitamin B₆deficiency

et al. 2005

View full text Add to dashboard Cite

The mechanism of pellagrous changes in skin caused by a deficiency of vitamin B6 was studied in respect to neogenesis of proline in skin collagen and glucose metabolism. In vitamin B6 deficiency the insulin/glucagon coefficient in serum decreased significantly from 3.02 to 2.32, indicating a metabolic change towards gluconeogenesis. A deficiency of vitamin B6 caused a decrease in the levels of vitamin B6-dependent enzymes, such as ornithine aminotransferase, alanine aminotransferase, and aspartate aminotransferase, which also contribute to gluconeogenesis. Because the conversion of ornithine to proline via pyrroline-5-carboxylate was suppressed due to the decrease in ornithine aminotransferase activity, the amount of proline in the skin collagen fraction also decreased significantly in vitamin B6-deficient rats compared with the pair-fed control. These results suggest that the pellagrous lesions in vitamin B6-deficiency are caused by an impaired synthesis of proline from ornithine, which results in the suppression of collagen neogenesis in the skin.

show abstract

Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment.

Cited by 95 publications

References 0 publications

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

Neurotransmitters partially restore glucose sensitivity of insulin and glucagon secretion from perfused streptozotocin-induced diabetic rat pancreas

Changes of glucose metabolism and skin-collagen neogenesis in vitamin B₆deficiency

Contact Info

Product

Resources

About

Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment.

Cited by 95 publications

References 0 publications

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

Expression of insulin receptor on clonal pancreatic alpha cells and its possible role for insulin-stimulated negative regulation of glucagon secretion

Neurotransmitters partially restore glucose sensitivity of insulin and glucagon secretion from perfused streptozotocin-induced diabetic rat pancreas

Changes of glucose metabolism and skin-collagen neogenesis in vitamin B6deficiency

Contact Info

Product

Resources

About

Changes of glucose metabolism and skin-collagen neogenesis in vitamin B₆deficiency