Glomerular filtration is reduced by high tidal volume ventilation in an in vivo healthy rat model

Abstract

Mechanical ventilation has been associated with organ failure in patients with acute respiratory distress syndrome. The present study examines the effects of tidal volume (V T) on renal function using two V T values (8 and 27 mL/kg) in anesthetized, paralyzed and mechanically ventilated male Wistar rats. Animals were randomized into two groups of 6 rats each: V T8 (V T, 8 mL/kg; 61.50 ± 0.92 breaths/min; positive end-expiratory pressure, 3.0 cmH2O; peak airway pressure (PAW), 11.8 ± 2.0 cmH2O), and V T27 (V T, 27 mL/kg; 33.60 ± 1.56 breaths/min; positive end-expiratory pressure, none, and PAW, 22.7 ± 4.0 cmH2O). Throughout the experiment, mean PAW remained comparable between the two groups (6.33 ± 0.21 vs 6.50 ± 0.22 cmH2O). For rats in the V T27 group, inulin clearance (mL·min-1·body weight-1) decreased acutely after 60 min of mechanical ventilation and even more significantly after 90 min, compared with baseline values (0.60 ± 0.05 and 0.45 ± 0.05 vs 0.95 ± 0.07; P < 0.001), although there were no differences between groups in mean arterial pressure or gasometric variables. In the V T8 group, inulin clearance at 120 min of mechanical ventilation remained unchanged in relation to baseline values (0.72 ± 0.03 vs 0.80 ± 0.05). The V T8 and V T27 groups did not differ in terms of serum thiobarbituric acid reactive substances (3.97 ± 0.27 vs 4.02 ± 0.45 nmol/mL) or endothelial nitric oxide synthase expression (94.25 ± 2.75 vs 96.25 ± 2.39%). Our results show that glomerular filtration is acutely affected by high tidal volume ventilation but do not provide information about the mechanism

N-acetylcysteine prevents pulmonary edema and acute kidney injury in rats with sepsis submitted to mechanical ventilation

“…In a recent study (16) conducted in our laboratory with healthy Wistar rats, we demonstrated that, after 120 min of mechanical ventilation at a low tidal volume (8 ml/kg BW), GFR remained unchanged in relation to baseline values. However, when we used a high tidal volume (27 ml/kg BW), GFR decreased after 60 min on mechanical ventilation, the reduction becoming more marked after 90 min (16). It is well established that mechanical ventilation can worsen renal hemodynamics and function (15), mainly when high volumes and high PEEP are used.…”

Effect of Triclosan on the Renal Cortex of Adult Male Albino Rats and the Possible Protective Role of Ellagic Acid: Histological and Biochemical Study

“…At the end of experiment, the rats were anesthetized with 50 mg/ kg.bw sodium pentobarbital intraperitoneally [19]. Blood samples were obtained directly from the heart by cardiac puncture.…”