Glomerular Alpha-1-Microglobulin in IgA Nephropathies

Murakami, T.; Kawakami, Hatsumi; Kobayashi, Kunihiko; Itoh, Yoshio

doi:10.1159/000168007

Cited by 2 publications

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Section: Table III the Identified Proteins Of Representative Spots Onmentioning

confidence: 97%

“…Itoh and coworkers (15) have reported that ␣ 1 -microglobulin was stained by immunofluorescence in the mesangial regions in IgA nephropathy renal biopsy. The most characteristic pathological finding in IgA nephropathy is the mesangial region where IgA deposits are predominantly found (1,15). To elucidate the pathology of IgA nephropathy, much attention has been paid to IgA itself, such as the abnormality of the IgA carbohydrate chain (1), but the importance of ␣ 1 -microglobulin may have been overlooked.…”

Section: Table III the Identified Proteins Of Representative Spots Onmentioning

confidence: 97%

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Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Hiramoto

Okada

Kanno

et al. 2007

Molecular & Cellular Proteomics

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To search for biomarkers of IgA nephropathy, protein profiles of urine samples from patients with IgA nephropathy and normal volunteers were compared using twodimensional DIGE. Most of the 172 spots identified in the urine were serum proteins, and their amounts in IgA nephropathy urine were much higher than those in normal urine; this can be explained as proteinuria caused by glomerular dysfunction. However, only ␣ 1 -microglobulin, also one of the major serum proteins, in IgA nephropathy urine was not higher in amount than that in normal urine. We confirmed using ELISA analysis that the amounts of transferrin and albumin in IgA nephropathy and diabetic nephropathy urine were much higher than those in normal urine, whereas the amount of ␣ 1 -microglobulin in IgA nephropathy urine was not higher than that in normal urine and was much lower than that in diabetic nephropathy urine. Approximately 50% of ␣ 1 -microglobulin forms a complex with IgA in serum. These results suggest that ␣ 1 -microglobulin in IgA nephropathy urine is a characteristic protein and might be a biomarker for IgA nephropathy and that ␣ 1 -microglobulin might have a relationship with IgA nephropathy pathology. Molecular & Cellular Proteomics 6:738 -744, 2007.

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Section: Table III the Identified Proteins Of Representative Spots Onmentioning

confidence: 97%

Section: Table III the Identified Proteins Of Representative Spots Onmentioning

confidence: 97%

Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Hiramoto

Okada

Kanno

et al. 2007

Molecular & Cellular Proteomics

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Human α₁‐microglobulin: Its measurement and clinical significance

Itoh

Kawai

1990

Clinical Laboratory Analysis

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alpha 1-Microglobulin (alpha 1-M), also called protein HC, is a low-molecular-weight (LMW) glycoprotein (about 30 kDa) with unique physicochemical properties. Using purified urinary alpha 1-M a standard and specific antibody against alpha 1-M, an assay system for alpha 1-M was developed, and the clinical significance of this protein was investigated by measuring total levels of alpha 1-M under physiological and pathological conditions. alpha 1-M is distributed in various body fluids: in serum, it consists mainly of free LMW alpha 1-M and monomeric IgA-alpha 1-M complex. The total alpha 1-M level in serum and urine usually reflects LMW alpha 1-M variation sensitively, and its determination is quite useful as an indicator of renal glomerulotubular dysfunction and hepatic dysfunction. Serum levels can vary, depending on IgA-alpha 1-M complex level, in parallel with the IgA concentration. The heterogeneity of alpha 1-M purified from different sources of urine by different procedures and underestimation of IgA-alpha 1-M complex by solid-phase antibody assays can be important causes for the discrepancy of serum levels between assays.

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Glomerular Alpha-1-Microglobulin in IgA Nephropathies

Cited by 2 publications

References 4 publications

Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Human α₁‐microglobulin: Its measurement and clinical significance

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Glomerular Alpha-1-Microglobulin in IgA Nephropathies

Cited by 2 publications

References 4 publications

Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Absence of Increased α1-Microglobulin in IgA Nephropathy Proteinuria

Human α1‐microglobulin: Its measurement and clinical significance

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Human α₁‐microglobulin: Its measurement and clinical significance