Globin RNA precursor molecules: Biosynthesis and processing in erythroid cells

Bastos, Roberto N.; Aviv, Haim

doi:10.1016/0092-8674(77)90081-2

Cited by 182 publications

(55 citation statements)

References 26 publications

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“…Considerable evidence also suggests that the mRNA in eukaryotic cells is generated by the post-transcriptional cleavage of larger nuclear RNA (2,3,6). Compelling results now indicate that viral mRNA from the DNA tumor viruses simian virus 40 (7) and adenovirus (8-11) and -the cellular mRNA for globin (12)(13)(14)(15) are processed from substantially larger nuclear RNA. However, all mRNA in eukaryotic cells may not be processed from large nuclear RNA.…”

Section: Introductionmentioning

confidence: 79%

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Gilmore-Hebert¹,

Wall²

1978

Proc. Natl. Acad. Sci. U.S.A.

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Recombinant DNA probes, produced by the molecular cloning of immunoglobulin K light chain mRNA, have been used to analyze heterogeneous nuclear RNA for presumptive precursors to cytoplasmic K light chain mRNA. Three discrete classes of nuclear RNA containing K mRNA sequences were detected after pulse-labeling of immunoglobulin-producing P3 myeloma cells. Two of these were substantially larger than K mRNA (approximately 10 and 4 times larger); the third was similar in size to K mRNA. Beginning with the largest, the sequential appearance of these three classes of nuclear RNA preceded the first a pearance of newly synthesized K light chain mRNA in the cytoplasm. The results presented here suggest that immunoglobulin K light chain mRNA is generated by the stepwise cleavage and processing of a large nuclear RNA transcript. Resolution of the events in the biogenesis of mRNA is essential to an understanding of the regulation of gene expression in eukaryotic cells. It is now well established that most mRNAs in eukaryotic cells are derived from nuclear RNA through post-transcriptional modifications including poly(A) addition (1-3), methylation, and the addition of 5'-terminal "caps" of the general structure m7G(5')pppN'-m-N"(m)p (4-6). Considerable evidence also suggests that the mRNA in eukaryotic cells is generated by the post-transcriptional cleavage of larger nuclear RNA (2,3,6). Compelling results now indicate that viral mRNA from the DNA tumor viruses simian virus 40 (7) and adenovirus (8-11) and -the cellular mRNA for globin (12)(13)(14)(15) are processed from substantially larger nuclear RNA. However, all mRNA in eukaryotic cells may not be processed from large nuclear RNA. RNA molecules larger than the respective cytoplasmic mRNA were not detected for ovalbumin mRNA (16) and murine RNA tumor virus RNA (17). However, short-lived nuclear RNAs, particularly those only marginally larger than their respective cytoplasmic mRNAs, may not be detected under the labeling and hybridization conditions generally used. In the case of silk fibroin mRNA, pulse-labeling at reduced temperature (presumably lowering the rate of RNA cleavage) was necessary to reveal a short-lived presumptive precursor only slightly larger than the silk fibroin mRNA (18)(19)(20) The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. K light chain mRNA* by procedures previously established for the preparation of recombinant DNA clones from rabbit globin mRNA (22). Using cloned immunoglobulin light chain recombinant plasmid DNA, we have detected discrete classes of rapidly labeled nuclear RNA containing K mRNA sequences which, after stringent denaturation (25, 26), remain substantially larger than the K light chain mRNA in immunoglobulin-producing mouse myeloma cells. Kinetic labeling studies indicate that immunoglobulin K light chain mRNA appears to be processed from a large nuc...

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Section: Introductionmentioning

confidence: 79%

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Gilmore-Hebert¹,

Wall²

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…At steady state it accounts for ~2000 molecules/cell. However, discrete populations of globin pre-mRNA of ~28 S have also been detected [10][11][12][13].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Precursors of distinct size for chicken α^A, α^D and β globin mRNAs

Therwath

Scherrer

1982

FEBS Letters

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“…Transcription has been detected both in a microlocus fragment containing HS1-HS4 linked together and in HS2 as part of a chimeric construct, raising the possibility that the ␤-globin LCR is transcribed (Collis et al 1990;Tuan et al 1992). In addition, the existence of large transcripts from the avian and murine ␤-globin clusters, up to seven times longer than the globin mRNAs, was described over two decades ago (Imaizumi et al 1973;Bastos and Aviv 1977). Although the human ␤-globin cluster represents one of the best characterized gene loci in eukaryotes, the extent of transcription throughout the locus has not been directly investigated.…”

mentioning

confidence: 99%

Intergenic transcription and transinduction of the human β-globin locus

Ashe¹,

Monks²,

Wijgerde³

et al. 1997

Genes Dev.

319

238

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We have identified novel nuclear transcripts in the human ␤-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surprisingly, transient transfection of a ␤-globin gene (⑀, ␥, or ␤) induces transcription of the LCR and intergenic regions from the chromosomal ␤-globin locus in nonerythroid cell lines. The ␤-globin genes themselves, however, remain transcriptionally silent. Induction is dependent on transcription of the globin gene in the transfected plasmid but does not require protein expression. Using in situ hybridization analysis, we show that the plasmid colocalizes with the endogenous ␤-globin locus providing insight into the mechanism of transinduction.

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Globin RNA precursor molecules: Biosynthesis and processing in erythroid cells

Cited by 182 publications

References 26 publications

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Precursors of distinct size for chicken α^A, α^D and β globin mRNAs

Intergenic transcription and transinduction of the human β-globin locus

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Globin RNA precursor molecules: Biosynthesis and processing in erythroid cells

Cited by 182 publications

References 26 publications

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Immunoglobulin light chain mRNA is processed from large nuclear RNA.

Precursors of distinct size for chicken αA, αD and β globin mRNAs

Intergenic transcription and transinduction of the human β-globin locus

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Product

Resources

About

Precursors of distinct size for chicken α^A, α^D and β globin mRNAs