Globally disseminated human pathogenic Escherichia coli of O25b‐ST131 clone, harbouring bla_CTX‐M‐15, found in Glaucous‐winged gull at remote Commander Islands, Russia

Abstract

With focus on environmental dissemination of antibiotic resistance among clinically relevant bacteria, such as the rising ESBL type of resistance among Escherichia coli, we investigated antibiotic resistance levels in wild birds in the Commander Islands and Kamchatka, Russia. Despite overall low resistance levels in randomly selected E. coli (one from each sample), we found multi-resistant ESBL-producing E. coli harbouring blaCTX-M-14 and blaCTX-M-15 using selective screening. Among these multi-resistant ESBL-producing E. coli we found one blaCTX-M-15 harbouring strain belonging to the O25b-ST131 clone, recognized for its clonal disseminated worldwide as a human pathogen. The potential in acquiring resistant bacteria of human origin, especially highly pathogenic clones, as well as downstream consequences of that, should not be underestimated but further investigated.

Dogs of Nomadic Pastoralists in Northern Kenya Are Reservoirs of Plasmid-Mediated Cephalosporin- and Quinolone-Resistant Escherichia coli, Including Pandemic Clone B2-O25-ST131

“…Overall, 36 (70%) of the ESBL-producing isolates belonged to either cluster K or L. Isolates with identical PFGE profiles were detected from 8 dogs and 2 humans, and closely related (Ͼ95% similarity) isolates were found in 1 human and 1 cat sampled in the same town. Three dogs were found to harbor isolates of The findings of this study corroborate the recognized worldwide spread of CTX-M-15-producing isolates, including the O25-ST131 clone, even in very remote areas where extended spectrum cephalosporins are used very rarely (9,13,16). The bla CTX-M-15 gene was found on large conjugative plasmids together with genes encoding resistance to other groups of antibiotics.…”

Human-Associated Extended-Spectrum β-Lactamase in the Antarctic

“…Genotyping by MLST corroborated the association with human gastrointestinal biota, as the majority of the STs belonged to widespread diseaseassociated genotypes, including E. coli ST131 carrying the bla CTX-M-15 gene, a worldwide disseminated human clinical clone (11).These findings constitute the first records of ESBLproducing bacteria in Antarctica, and given that they were isolated from all the sampled bases it seems that humanassociated bacteria are discharged into the environment more than occasionally. The emergence of CTX-M has been referred to as one of the most striking examples of rapid, global dissemination of plasmid-mediated resistance determinants among bacterial pathogens (16).…”

Temporal variations in patterns ofEscherichia colistrain diversity and antimicrobial resistance in the migrant Egyptian vulture

“…The near-absence of studies of the of E. coli in wild vertebrates in densely-populated countries is worrisome especially given the increased risk of wild species interacting with humans and domestic animals, and are urgently required [4]. In particular, documentation of the temporal and spatial patterns of antimicrobial drug resistance in different habitats and species in developing countries is essential to developing a cogent understanding of wildlife as a source of antimicrobial resistance [16,17]…”