Global transcriptome analysis of the E. coli O157 response to Agrimonia pilosa extract

Abstract

Antibiotics are certainly beneficial to treat the bacterial infection. However, inappropriate use puts patients at danger for avoidable adverse drug reactions. This inappropriate use accelerates the emergence of resistance and potentially increases overall health-care costs. In oriental pharmacology, herbal medicines had been used as an antibiotic drug, but its antibiotic mechanism has not yet been proved completely. Here, we studied the antibiotic effects of Agrimonia pilosa on E. coli O157:H7. Especially, it is noteworthy that the antibiotic efficacy of the herb extract on three proven targets for a main antibiotic drug: bacterial cell wall biosynthesis, protein synthesis and DNA replication and repair. The results show the antibiotic effects through the inhibition of bacterial cell wall synthesis, folic acid synthesis, and multidrug efflux.

The Multi‐Targeted Effects of Chrysanthemum Herb Extract Against Escherichia coli O157:H7

“…The results demonstrate that the gene regulation of the E. coli O157 was affected by the CL MC fraction. The functional categories were sourced from the NCBI genome database gene classification based on the categories of orthologous genes (Choi et al ., ; Kim et al ., ; Kim et al ., ; Yang et al ., ). A total of 824 genes (15.40% of all expressed genes) in the E. coli O157 genome were up‐ or down‐regulated by at least two‐fold ( P ≤ 0.07) under the CL MC fraction treatment.…”

“…A disk diffusion method was utilized to examine the antibiotic capabilities of the CL extracts (Choi et al ., ; Kim et al ., ; Kim et al ., ; Yang et al ., ). The prepared E. coli O157 cells within the LB broth were spread onto a LB agar plate, and a sterilized 6 mm filtered paper disk was placed on the plate tightly where the E. coli O157 was spread.…”

“…A microarray was performed according to the following procedure (Choi et al ., ; Kim et al ., ; Kim et al ., ; Yang et al ., ). cDNA was synthesized from 12 µg of the total RNA using random primers (Invitrogen, Carlsbad, CA) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA) following the Agilent E. coli 8 × 15 K arrays protocol (Agilent Technologies, Palo Alto, CA).…”