Global Transcriptome Analysis of<i>Tropheryma whipplei</i>in Response to Temperature Stresses

Crapoulet, Nicolas; Barbry, Pascal; Raoult, Didier; Renesto, Patricia

doi:10.1128/jb.00507-06

Cited by 31 publications

(30 citation statements)

References 62 publications

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“…Homologues of enzymes involved in the utilization of carbohydrates and enzymes of the Entner Doudoroff and pentose phosphate pathways are present in the H. pylori genome, suggesting that glucose can be used as a source of energy. Our study revealed that most genes involved in energy metabolism of H. pylori were up-regulated, which was consistent to other studies (Crapoulet et al, 2006). The increased levels of energy generation indicate that the cells incubated at low temperature are stressed by energy limitation.…”

Section: Helicobacter Pylori Expression Microarray Temperaturesupporting

confidence: 94%

“…But recently, an increasing number of reports suggest that heat shock proteins can be induced by cold shock, and that the proper folding of proteins, as well as the refolding of cold-damaged proteins, is important for bacterial growth during cold shock (Phadtare et al, 2004;Crapoulet et al, 2006;Lee et al, 2008). Under physiological conditions, DnaK represents the intracellular stress sensor.…”

Section: Helicobacter Pylori Expression Microarray Temperaturementioning

confidence: 99%

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Gene expression profile of Helicobacter pylori in response to growth temperature variation

et al. 2009

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A Helicobacter pylori whole-genome DNA microarray was constructed to study expression profiles of H. pylori in response to a sudden temperature transfer from 37 degrees C to 20 degrees C. The expression level of the genome at each of four time points (15, 30, 60, and 120 min) after temperature downshift was compared with that just before cold treatment. Globally, 10.2 % (n=167) of the total predicted H. pylori genes (n=1636) represented on the microarray were significantly differentially expressed (p<0.05) over a 120 min period after shift to low temperature. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated by quantitative real-time PCR. Up-regulated genes mainly included genes involved in energy metabolism and substance metabolism, cellular processes, protein fate, ribosomal protein genes, and hypothetical protein genes, which indicate the compensational responses of H. pylori to temperature downshift. Those genes play important roles in adaption to temperature downshift of H. pylori. Down-regulation of DNA metabolism genes and cell envelope genes and cellular processes genes may reflect damaged functions under low temperature, which is unfavorable to bacterial infection and propagation. Overall, this time-course study provides new insights into the primary response of H. pylori to a sudden temperature downshift, which allow the bacteria to survive and adapt to the new host environment.

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Section: Helicobacter Pylori Expression Microarray Temperaturesupporting

confidence: 94%

Section: Helicobacter Pylori Expression Microarray Temperaturementioning

confidence: 99%

Gene expression profile of Helicobacter pylori in response to growth temperature variation

et al. 2009

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“…Vaccine development is unnecessary due to the low prevalence of WD, even taking into account its apparent underdiagnosis [1]. The research of specific markers of WD has been undertaken in the fields of genetics [27], proteomics [28,29] and immunology [30][31][32] (Table 2). …”

Section: Environmental Origin Of T Whipplei and Asymptomatic Carriersmentioning

confidence: 99%

Advances in Tropheryma Whipplei Research: The Rush to Find Biomarkers for Whipple‘s Disease

Kowalczewska¹,

Raoult

2007

Future Microbiol.

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Whipple's disease (WD) is a systemic chronic infection, caused by the Gram-positive bacterium Tropheryma whipplei. There are several clinical traits linked to WD: histological lesions in the GI tract in association with diverse clinical manifestations (classic WD), endocarditis with negative blood cultures, and isolated neurological infection. WD is rare, predominantly affects middle-aged men and is fatal without treatment. The most recent strategy for diagnosing WD uses the results of diastase-resistant periodic acid Schiff staining and PCR in parallel, both performed on involved organ/tissue biopsy (small intestine, cardiac valve and cerebrospinal fluid). The generation of rabbit polyclonal antibodies has enabled the detection of the bacterium in tissues by immunohistochemical staining. However, the diagnosis of WD remains an invasive procedure. The recent achievement of stable bacterial culture and sequencing of the T. whipplei genome has opened a framework for the development of a biomarker platform. Several studies in different fields have been performed, for example, transcriptomics, immunoproteomics and comparative proteomics. Biomarker candidates have been proposed for the development of less invasive procedures for diagnosing WD.

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“…Nowadays, the high-throughput transcriptome sequencing (RNA-seq) technique has dramatically accelerated the analytical capacity and has been widely used in constructing global networks of gene expression (16). In the past decades, there were several comparative transcriptome analyses focused on temperature-dependent genes in some important environmental bacteria, including Escherichia coli (12), Tropheryma whipplei (13), Pseudomonas aeruginosa (14), Streptococcus thermophilus (17), and Bacillus cereus (18). The expression patterns of virulence-related genes in Listeria monocytogenes, Salmonella enterica, Xanthomonas oryzae, and V. cholerae have also been revealed through transcriptome analysis (19)(20)(21)(22).…”

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confidence: 99%

“…Hence, temperature has been considered one of the crucial environmental factors that can regulate the metabolic process, growth, adhesion, and even pathogenicity of bacteria by influencing their gene expression (12)(13)(14). V. splendidus strain JZ6 was previously isolated and identified as a pathogenic agent for Yesso scallop (Patinopecten yessoensis) in low-temperature environments (5).…”

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confidence: 99%

Comparative Transcriptome Analysis of Vibrio splendidus JZ6 Reveals the Mechanism of Its Pathogenicity at Low Temperatures

Chen

Zhang

et al. 2016

Appl Environ Microbiol

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dYesso scallop-pathogenic Vibrio splendidus strain JZ6 was found to have the highest virulence at 10°C, while its pathogenicity was significantly reduced with increased temperature and completely incapacitated at 28°C. In the present study, comparative transcriptome analyses of JZ6 and another nonpathogenic V. splendidus strain, TZ19, were conducted at two crucial culture temperatures (10°C and 28°C) in order to determine the possible mechanism of temperature regulation of virulence. Comparisons among four libraries, constructed from JZ6 and TZ19 cultured at 10°C and 28°C (designated JZ6_10, JZ6_28, TZ19_10, and TZ19_28), revealed that 241 genes were possibly related to the increased virulence of JZ6 at 10°C. There were 10 genes, including 2 encoding Flp pilus assembly proteins (FlhG and VS_2437), 6 encoding proteins of the "Vibrio cholerae pathogenic cycle" (ToxS, CqsA, CqsS, RpoS, HapR, and Vsm), and 2 encoding proteins in the Sec-dependent pathway (SecE and FtsY), that were significantly upregulated in JZ6_10 (P < 0.05) compared to those in JZ6_28, TZ19_10, and TZ19_28, which were supposed to be responsible for adhesion, quorum sensing, virulence, and protein secretion of V. splendidus. When cultured at 10°C, JZ6 cells were larger and tended to aggregate more than those cultured at 28°C. The virulence factor (extracellular metalloprotease) was also found to be highly expressed in the extracellular product (ECP) of JZ6 at 10°C, and this ECP exhibited obvious cytotoxicity to oyster primary hemocytes, A549 cells, and L929 cells. These results indicated that low temperatures (10°C) could enhance adhesion, activate the quorum sensing systems, upregulate virulence factor synthesis and secretion, and, lastly, increase the pathogenicity of JZ6.T he Gram-negative pathogenic bacterium Vibrio splendidus is a ubiquitous and representative species of the Vibrio genus, a causal agent of vibriosis, which causes high rates of mortality in aquaculture animals, including turbot, scallop, clam, and oyster (1-5). Like most Vibrio strains, V. splendidus is an opportunistic pathogen that causes mortality of animals in an optimum environment, whereas it usually acts as a "normal bacterium" in the host or environment under adverse conditions (6-8). Therefore, environmental factors are important regulators of the pathogenicity of V. splendidus.In previous studies, most opportunistic pathogenic Vibrio bacteria, such as V. splendidus, V. cholerae, V. parahaemolyticus, V. alginolyticus, and V. harveyi, have been reported to cause high rates of mortality of cultured animals in the summer and enter a viable-but-nonculturable (VBNC) state when they are exposed to temperatures below 10°C (6, 8-11). Hence, temperature has been considered one of the crucial environmental factors that can regulate the metabolic process, growth, adhesion, and even pathogenicity of bacteria by influencing their gene expression (12-14). V. splendidus strain JZ6 was previously isolated and identified as a pathogenic agent for Yesso scallop (Patinopecten yessoensis...

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Global Transcriptome Analysis ofTropheryma whippleiin Response to Temperature Stresses

Cited by 31 publications

References 62 publications

Gene expression profile of Helicobacter pylori in response to growth temperature variation

Gene expression profile of Helicobacter pylori in response to growth temperature variation

Advances in Tropheryma Whipplei Research: The Rush to Find Biomarkers for Whipple‘s Disease

Comparative Transcriptome Analysis of Vibrio splendidus JZ6 Reveals the Mechanism of Its Pathogenicity at Low Temperatures

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