Global transcriptome analysis of AtPAP2 - overexpressing Arabidopsisthalianawith elevated ATP

Abstract

BackgroundAtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes.ResultsATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription.ConclusionsAtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.

Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development

“…Increased adenylate pools have previously been demonstrated to increase the potato tuber yield in experiments in which the activity of the plastidial adenylate kinase was altered [26]. ATP and adenylate pools were shown to be significantly increased in the rosette leaves of AtPAP2 OE Arabidopsis lines [9]. Meanwhile, the expression levels and enzyme activity of SPS were also significantly elevated in the AtPAP2 OE lines, which could be expected to supply more sucrose for export and anabolism.…”

“…Primers (Table S1) were designed according to published papers [8] and produce 50‐ to 150‐bp amplicon using Primer5 software. Quantitative RT‐PCR data were corrected by calculation of the PCR efficiency individually using the LinReg PCR software [9].…”

Exaggerated root respiration accounts for growth retardation in a starchless mutant of Arabidopsis thaliana

“…We assume a similar decoupling of respiratory activity and ADP phosphorylation to occur in pgm roots, as we did not observe phenotypic alterations of roots displaying an elevated ATP/ADP ratio, e.g. increased root/shoot ratio (Carrari et al ., ) or enhanced nitrate uptake (Sun et al ., ).…”

Two pivotal RNA editing sites in the mitochondrial atp1mRNA are required for ATP synthase to produce sufficient ATP for cotton fiber cell elongation

“…Sun et al . () reported that overexpression lines of AtPAP2 contained higher levels of ATP, and the overexpression lines grew faster, produced more seeds, and contained higher concentrations of leaf sucrose and glucose. The higher energy supply may affect the regulation of many transcripts and metabolites which enhance plant growth.…”