Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system

Pumirat, Pornpan; Cuccui, Jon; Stabler, Richard A.; Stevens, Joanne M.; Muangsombut, Veerachat; Singsuksawat, Ekapot; Stevens, Mark P.; Wren, Brendan W.; Korbsrisate, Sunee

doi:10.1186/1471-2180-10-171

Cited by 30 publications

(55 citation statements)

References 38 publications

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“…We also found that high salinity and high proportion of gravel were associated with the presence of B. pseudomallei (see Supplementary Information Figure 5). The association between presence of B. pseudomallei and high soil salinity is consistent with previous laboratory studies 22 . Although our model did not find an association between the presence of B. pseudomallei and soil pH reported by previous environmental studies 23 , this could be because soil pH is generally associated with other soil factors, particularly soil salinity 21 , reducing the capacity of our model to identify this as a geographic risk factor.…”

supporting

confidence: 92%

Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis

et al. 2016

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Burkholderia pseudomallei, a highly pathogenic bacterium that causes melioidosis, is commonly found in soil in Southeast Asia and Northern Australia1,2. Melioidosis can be difficult to diagnose due to its diverse clinical manifestations and the inadequacy of conventional bacterial identification methods3. The bacterium is intrinsically resistant to a wide range of antimicrobials, and treatment with ineffective antimicrobials may result in case fatality rates (CFRs) exceeding 70%4,5. The importation of infected animals has, in the past, spread melioidosis to non-endemic areas6,7. The global distribution of B. pseudomallei and burden of melioidosis, however, remain poorly understood. Here, we map documented human and animal cases, and the presence of environmental B. pseudomallei, and combine this in a formal modelling framework8-10 to estimate the global burden of melioidosis. We estimate there to be 165,000 (95% credible interval 68,000-412,000) human melioidosis cases per year worldwide, of which 89,000 (36,000-227,000) die. Our estimates suggest that melioidosis is severely underreported in the 45 countries in which it is known to be endemic and that melioidosis is likely endemic in a further 34 countries which have never reported the disease. The large numbers of estimated cases and fatalities emphasise that the disease warrants renewed attention from public health officials and policy makers.

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confidence: 92%

Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis

et al. 2016

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“…Genes differentially regulated in Bp isolated from infected mouse lungs were significantly enriched in 9 condition-dependent clusters (, hypergeometric test). One upregulated cluster C030, comprised T3SS3 genes, likely reflecting a strong functional requirement for T3SS3 activity during lung colonization [42]. Among the upregulated genes, we identified five that might function as potential effector proteins - BPSS1498 ( tssD-5 ), BPSL3319 ( fliC ), BPSS1525 ( bopE ), BPSS1529 ( bipD ) and BPSS1532 ( bipB ).…”

Section: Resultsmentioning

confidence: 99%

The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei

et al. 2013

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Burkholderia pseudomallei (Bp), the causative agent of the often-deadly infectious disease melioidosis, contains one of the largest prokaryotic genomes sequenced to date, at 7.2 Mb with two large circular chromosomes (1 and 2). To comprehensively delineate the Bp transcriptome, we integrated whole-genome tiling array expression data of Bp exposed to >80 diverse physical, chemical, and biological conditions. Our results provide direct experimental support for the strand-specific expression of 5,467 Sanger protein-coding genes, 1,041 operons, and 766 non-coding RNAs. A large proportion of these transcripts displayed condition-dependent expression, consistent with them playing functional roles. The two Bp chromosomes exhibited dramatically different transcriptional landscapes — Chr 1 genes were highly and constitutively expressed, while Chr 2 genes exhibited mosaic expression where distinct subsets were expressed in a strongly condition-dependent manner. We identified dozens of cis-regulatory motifs associated with specific condition-dependent expression programs, and used the condition compendium to elucidate key biological processes associated with two complex pathogen phenotypes — quorum sensing and in vivo infection. Our results demonstrate the utility of a Bp condition-compendium as a community resource for biological discovery. Moreover, the observation that significant portions of the Bp virulence machinery can be activated by specific in vitro cues provides insights into Bp's capacity as an “accidental pathogen”, where genetic pathways used by the bacterium to survive in environmental niches may have also facilitated its ability to colonize human hosts.

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“…Our results supported the results of a previous study that demonstrated that saline soil and water were the preferred growth conditions for B. pseudomallei in northeastern Thailand. 21 Moreover, the growth-promoting effect of iron on B. pseudomallei reinforced the significant association between B. pseudomallei-positive sites and the soil being reddish gray or reddish brown, indicating its iron content and the presence of animals. 19 Red to reddishyellow loamy sand containing iron and iron-impregnated nodules has been reported in the melioidosis-endemic area of Nam Phong catena in northeastern Thailand.…”

Section: Discussionmentioning

confidence: 84%

“…17 The association of the presence of B. pseudomallei with that of iron and salt in water and soil has been demonstrated in endemic areas. 16,[18][19][20][21][22] These results may facilitate understanding of the ability of B. pseudomallei to adapt to soil in northeastern Thailand that has different environmental features, including high salinity and the presence of iron. The resilience exhibited by B. pseudomallei in endemic areas has resulted in a high risk of pathogen exposure.…”

Section: Introductionmentioning

confidence: 99%

Morphological Alteration and Survival of Burkholderia pseudomallei in Soil Microcosms

Kamjumphol¹,

Chareonsudjai²,

Taweechaisupapong³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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The resilience of Burkholderia pseudomallei, the causative agent of melioidosis, was evaluated in control soil microcosms and in soil microcosms containing NaCl or FeSO4 at 30°C. Iron (Fe(II)) promoted the growth of B. pseudomallei during the 30-day observation, contrary to the presence of 1.5% and 3% NaCl. Scanning electron micrographs of B. pseudomallei in soil revealed their morphological alteration from rod to coccoid and the formation of microcolonies. The smallest B. pseudomallei cells were found in soil with 100 μM FeSO4 compared with in the control soil or soil with 0.6% NaCl (P < 0.05). The colony count on Ashdown's agar and bacterial viability assay using the LIVE/DEAD® BacLight™ stain combined with flow cytometry showed that B. pseudomallei remained culturable and viable in the control soil microcosms for at least 120 days. In contrast, soil with 1.5% NaCl affected their culturability at day 90 and their viability at day 120. Our results suggested that a low salinity and iron may influence the survival of B. pseudomallei and its ability to change from a rod-like to coccoid form. The morphological changes of B. pseudomallei cells may be advantageous for their persistence in the environment and may increase the risk of their transmission to humans.

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Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system

Cited by 30 publications

References 38 publications

Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis

Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis

The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei

Morphological Alteration and Survival of Burkholderia pseudomallei in Soil Microcosms

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