Global transcriptional characterization of SP and MP cells from the myogenic C2C12 cell line: effect of FGF6

Decraene, Charles; Benchaouir, Rachid; Dillies, Marie‐Agnès; Israeli, David; Bortoli, Sylvie; Rochon, Christelle; Rameau, Philippe; Pitaval, Amandine; Roux, Diana Tronik‐Le; Danos, Olivier; Gidrol, Xavier; Garcı́a, Luis; Piétu, Geneviève

doi:10.1152/physiolgenomics.00141.2004

Cited by 21 publications

(17 citation statements)

References 49 publications

(48 reference statements)

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“…These cells are refractory to Hoechst 33342 dye-staining due to the ABCG2 (BCRP1) transporter (9), and are resistant to certain drugs due to other ABC transporters (10). The SP phenotype was identified in each normal adult tissue, suggesting that SP is enriched in primitive and non-differentiated cells (11,12). On the other hand, concurrent studies have shown isolation of SP cells from various kinds of human solid cancers such as lung cancer, mesenchymal neoplasms, acute myelogenous leukemia, neuroblastoma, and glioma (13)(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Tumor initiating potential of side population cells in human gastric cancer

Saikawa¹

2009

Int J Oncol

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Abstract. Side population (SP) cells are a small subpopulation of cells with enriched source of gastric tumor-initiating cells (TICs) with stem-like cell property that are characterized by high efflux ability of Hoechst 33342 dye, reflecting high expression of several subtypes of the ATP-binding cassette transporter family that is characteristic of stem cells. The present study is the first to discover and characterize SP cells within gastric cancer (GC) tumors. In this study, human GC cell lines (MKN45, KATOIII, MKN74, MKN28 and MKN1) were analyzed using flow cytometry for SP cell isolation, and all GC cell lines showed a distinct fraction of SP cells, ranging from 0.02±0.001 to 1.93±0.16%. Among these cell lines, MKN45 cultures possessed the highest percentage of SP cells. Using MKN45 cells, we demonstrated stem cell-like characteristics of SP cells of the cell lines as a possible subpopulation with enriched TICs, as indicated by ABC transporter gene expression (MDR1 and BCPR1), chemo-resistance and tumorigenicity in vivo. In addition, we report the first identification and isolation of SP cells from clinical GC tissues as well as human GC cell lines. These SP cells demonstrate higher tumorigenicity in vivo than does the overall cell population in the parent tissue. In conclusion, we demonstrate that solid tumor tissue such as human GC contains TICs, with the existence of heterogeneity and distinct hierarchy in malignancy, suggesting the future possibility of a novel therapeutic tool targeting TICs for overcoming this malignant disease.

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Section: Introductionmentioning

confidence: 99%

Tumor initiating potential of side population cells in human gastric cancer

Saikawa¹

2009

Int J Oncol

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“…In a third methodology, fl ow cytometric isolation of "side population" (SP) cells, fi rst described by Goodell et al [42], permits the enrichment of cells with stem cell activity and distinctive expression of the ATP-binding cassette (ABC) transporters. SP cells are reportedly enriched in primitive, nondifferentiated cells [43,44], and recent studies have shown the existence of SP cells in human solid cancers [39,[45][46][47][48][49] (Fig. 5).…”

Section: Defi Nition Of Cancer Stem Cells (Cscs)mentioning

confidence: 95%

Gastric carcinogenesis and the cancer stem cell hypothesis

et al. 2010

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“…The characterization of cells within SP demonstrates that they are immature, poorly differentiated, and highly tumorigenic. Gene expression profiles of SP show that these cells are less differentiated than non-SP cells [100]. The ABC transporters may play three functions in CSCs.…”

Section: Cancer Stem Cells and Mdrmentioning

confidence: 98%