Global sperm deoxyribonucleic acid methylation is unaffected in protamine-deficient infertile males

Aoki, Vincent W.; Emery, Benjamin R.; Carrell, Douglas T.

doi:10.1016/j.fertnstert.2006.04.023

Cited by 39 publications

(30 citation statements)

References 22 publications

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“…Although differences in DNA methylation for normal and protamine deficient sperm cells was found not to be significant, such a methylation pattern has not been as thoroughly studied in the male pronucleus [Aoki et al 2006]. Therefore, initially, we compared the methylation level of pronuclei derived from normal and protamine deficient sperm cells, highlighting the importance of methylation during early stages of development.…”

Section: Discussionmentioning

confidence: 98%

“…We also observed an inverse relationship between sperm protamine deficiency and sperm motility parameters as well as morphology. Aoki et al [2006] showed that sperm count and motility decrease, and normal head morphology is impaired as the amount of protamine 1 and 2 decrease. It is well documented that the protamine deficiency can lead to a decrease in fertilization rate [Araki et al 2005].…”

Section: Discussionmentioning

confidence: 99%

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Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes

Rajabi

Mohseni-Kouchesfehani

Mohammadi‐Sangcheshmeh

et al. 2016

Systems Biology in Reproductive Medicine

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Epigenetic abnormalities and abnormal chromatin structure in sperm may lead to male infertility. Protamine deficiency is among the disorders of chromatin structure in sperm. The study of epigenetic changes in male pronuclei is necessary since abnormal sperm is sometimes used to create embryos using assisted reproductive techniques. The present study was carried out to compare epigenetic global marks in male pronuclei derived from normal and protamine deficient sperm cells. To do so, interspecies fertilization was used to obtain the male pronucleus. Normal and protamine deficient sperm cells, which were identified by chromomycin A3 staining, were injected into mouse oocytes. Oocytes were cultured until pronuclear formation and were then labeled with different antibodies (anti 5-methylcytosine, anti 5-hydroxymethylcytosine, and anti acetyl H4K12). Based on the fluorescence intensity, the level of each of these epigenetic factors was determined and they revealed a significant relationship between the level of sperm protamine deficiency and sperm epigenetic factors. Protamine deficiency was found to be associated with an increased methylation (p=0) and decreased hydroxymethylation rate (p=0.015) of the male pronucleus chromatin. However, no association was found between protamine deficiency and the level of H4K12 acetylation (p=0.548). Also, the efficiency of fertilization in protamine deficient sperm cells was less than normal. These results suggest that protamine deficient sperm cells lead to the formation of epigenetically altered pronuclei.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes

Rajabi

Mohseni-Kouchesfehani

Mohammadi‐Sangcheshmeh

et al. 2016

Systems Biology in Reproductive Medicine

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show abstract

“…Men with unequal protamine ratios have decreased semen quality, decreased fertilization ability, and DNA damage (112,120). Abnormal protamine ratios may also be associated with problems in epigenetic reprogramming and gene imprinting (111) and have been correlated with IVF success and embryo quality (121). An SNP, G197 T, was found in the gene that codes for P1.…”

Section: Epigenetic Errorsmentioning

confidence: 99%

“…Thus far, studies examining the global methylation patterns of sperm from infertile men have not found significant differences in comparison with normal men, but additional research is necessary to definitively confirm the role of imprinting and epigenetic information in infertility (121). The correlation between the incidence of imprinting disorders and ART in men with abnormal sperm is a controversial topic.…”

Section: Epigenetic Errorsmentioning

confidence: 99%

The genetic causes of male factor infertility: A review

O’Brien

Varghese²,

Agarwal

2010

Fertility and Sterility

400

258

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“…Imprinting errors have been identified in the sperm of oligozoospermic, teratozoospermic, and oligoasthenoteratozoospermic (OAT) men and in men with altered protamine ratios (12)(13)(14)(15)(16)(17)(18)(19)(20). Some studies have reported that global sperm DNA methylation levels in infertile men are similar to those in controls (16,18,21), whereas others have reported abnormal global DNA methylation in some classes of male infertility (22)(23)(24).…”

mentioning

confidence: 99%

Genome-wide sperm deoxyribonucleic acid methylation is altered in some men with abnormal chromatin packaging or poor in vitro fertilization embryogenesis

Aston

Punj

Liu

et al. 2012

Fertility and Sterility

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Global sperm deoxyribonucleic acid methylation is unaffected in protamine-deficient infertile males

Cited by 39 publications

References 22 publications

Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes

Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes

The genetic causes of male factor infertility: A review

Genome-wide sperm deoxyribonucleic acid methylation is altered in some men with abnormal chromatin packaging or poor in vitro fertilization embryogenesis

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