2021
DOI: 10.1016/j.xpro.2021.100991
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Global Run-On sequencing to measure nascent transcription in C. elegans

Abstract: Summary Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nematode Caenorhabditis elegans to measure transcription from embryos and adult worms. Here, we provide a detailed overview of the protocol highlighting the critical steps for gen… Show more

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Cited by 3 publications
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“…It has been demonstrated that CapSeq exhibits greater precision in identifying transcription start sites compared to GRO-seq specifically within the C. elegans model 48 . Both of these methods rely on nuclei isolation, which exhibits an efficiency of approximately 50% 50 . Consequently, they necessitate a substantial amount of initial material for analysis.…”
Section: Discussionmentioning
confidence: 99%
“…It has been demonstrated that CapSeq exhibits greater precision in identifying transcription start sites compared to GRO-seq specifically within the C. elegans model 48 . Both of these methods rely on nuclei isolation, which exhibits an efficiency of approximately 50% 50 . Consequently, they necessitate a substantial amount of initial material for analysis.…”
Section: Discussionmentioning
confidence: 99%