Global Regulation of the
            <i>Salmonella enterica</i>
            Serovar Typhimurium Major Porin, OmpD

Santiviago, Carlos A.; Toro, Carlos A.; Hidalgo, Alejandro A.; Youderian, Philip; Mora, Gabriela

doi:10.1128/jb.185.19.5901-5905.2003

Cited by 49 publications

(47 citation statements)

References 28 publications

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“…Salmonella OMP fraction was purifed by the method of Santiviago et al (2003) and analyzed by polyacrylamide gel electrophoresis as described previosuly (Bossi and Figueroa-Bossi 2007).…”

Section: Omp Preparationmentioning

confidence: 99%

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Figueroa‐Bossi¹,

Valentini²,

Malleret³

et al. 2009

Genes Dev.

206

254

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A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and characterized in the present study, encodes a conserved enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. In the absence of inducer, chiP is kept silent by the action of a constitutively made small RNA, ChiX (formerly SroB, RybC), which pairs with a sequence at the 59 end of chiP mRNA. Silencing is relieved in the presence of chitooligosaccharides due to the accumulation of an RNA that pairs with ChiX and promotes its degradation. Anti-ChiX RNA originates from an intercistronic region of the chb operon, which comprises genes for chitooligosaccharide metabolism and whose transcription is activated in the presence of these sugars. We present evidence suggesting that the chb RNA destabilizes ChiX sRNA by invading the stem of its transcription terminator hairpin. Overall, our findings blur the distinction between effector and target in sRNA regulation, raising the possibility that some of the currently defined targets could actually be inhibitors of sRNA function.[Keywords: Antisense regulation; chitoporin; chitobiose; Hfq; small RNA] Supplemental material is available at http://www.genesdev.org.

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“…Salmonella OMP fraction was purifed by the method of Santiviago et al (2003) and analyzed by polyacrylamide gel electrophoresis as described previosuly (Bossi and Figueroa-Bossi 2007).…”

Section: Omp Preparationmentioning

confidence: 99%

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Figueroa‐Bossi¹,

Valentini²,

Malleret³

et al. 2009

Genes Dev.

206

254

View full text Add to dashboard Cite

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“…OmpD is a trimeric porin with homology to OmpF and OmpC (9, 15) whose expression is enhanced by anaerobiosis and suppressed by low pH, whereas OmpF and OmpC expression is sensitive to changes in osmolarity (16). OmpD has an unclear role in infection whereas loss of OmpR is attenuating (17,18).…”

Section: Discussionmentioning

confidence: 99%

The porin OmpD from nontyphoidal Salmonella is a key target for a protective B1b cell antibody response

Gil‐Cruz

Bobat

Marshall

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

155

172

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“…OmpD is one of the most abundant outer membrane proteins of S. enterica serovar Typhimurium and is regulated by a multiplicity of environmental factors, including anaerobiosis, lower pH, and catabolite repression (33). As shown in showed a 4-fold elevation in resistance to ceftriaxone compared to strain 01-4; however, there was no difference in MIC value when ompC was deleted to give strain 01-4(⌬ompC).…”

Section: Discussionmentioning

confidence: 99%

The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

Hu¹,

Chen²,

Zhang³

et al. 2011

Antimicrob Agents Chemother

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Significant increases in STM3031, STM1530, and AcrD protein levels and significant decreases in OmpC and OmpD protein levels are present when the ceftriaxone-resistant Salmonella enterica serovar Typhimurium R200 strain is compared with the ceftriaxone-susceptible strain 01-4. AcrD is known to be involved in drug export, and STM3031 seems to play a key role in ceftriaxone resistance. Here, we examine the roles of STM1530, OmpC, and OmpD in ceftriaxone resistance. An ompD gene deletion mutant showed 4-fold higher ceftriaxone resistance than 01-4. An ompC gene deletion mutant showed 4-fold higher cephalothin and erythromycin resistance than 01-4, but there was no effect on ceftriaxone resistance. However, a stm1530 deletion mutant did show >64-fold lower ceftriaxone resistance than R200. Moreover, the STM3031 protein was significantly decreased in R200(⌬stm1530) compared to R200. STM3031 expression has been shown to be influenced by the two-component system regulator gene baeR. CpxR seems to modulate BaeR. A cpxA-cpxR gene deletion mutant showed >2,048-fold lower ceftriaxone resistance than R200. The outer membrane protein profile of R200(⌬cpxAR) showed significant decreases in STM3031 and STM1530 compared to R200, while OmpD had returned to the level found in 01-4. Furthermore, the stm3031, stm1530, and ompD mRNA levels were correlated with their protein expression levels in these strains, while decreases in the mRNA levels of the efflux pump acrB, acrD, and acrF genes were found in R200(⌬cpxAR). Findings similar to those for R200(⌬cpxAR) were found for R200(⌬baeSR). These results, together with those for STM3031 and the fact that STM1530 is an outer membrane protein, suggest that STM1530 and OmpD are influenced by the CpxAR and BaeSR two-component systems and that this contributes to S. enterica serovar Typhimurium ceftriaxone resistance.

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Global Regulation of the Salmonella enterica Serovar Typhimurium Major Porin, OmpD

Cited by 49 publications

References 28 publications

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

The porin OmpD from nontyphoidal Salmonella is a key target for a protective B1b cell antibody response

The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

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