Global proteomic mapping of alkali stress regulated molecular networks in Helianthus tuberosus L.

Zhang, Aiqin; Zang, Wei; Zhang, Xiyang; Ma, Yangyang; Yan, Xiufeng; Pang, Qiuying

doi:10.1007/s11104-016-2945-7

Cited by 27 publications

(10 citation statements)

References 75 publications

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“…In this study, the RPs decreased in both non‐AM seedlings under AS (AS vs. CK analysis) and AMF seedlings under AS (AS‐AM vs. AM analysis), indicating that protein synthesis was affected by AS (Tables S2 and S3). Similar results were also found in Helianthus tuberosus by Zhang et al (). Interestingly, we also found that eight small‐subunit and 22 large‐subunit RPs were up‐regulated after AMF inoculation under AS (AS‐AM vs. AS analysis; Table S5 and Figure ).…”

Section: Discussionsupporting

confidence: 90%

“…Salt‐alkaline soil is one of the major abiotic constraints that limit plant distribution and yield, and this soil will cover >50% of arable lands throughout the world by 2050 (Yu, Chen, Wang, Sun, & Dai, ; Zhang et al, ). In the northeast of China, alkaline meadows cover >70% of the land, and the area continues to expand (Lin, Wang, Sun, Mu, & Yan, ).…”

Section: Introductionmentioning

confidence: 99%

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Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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“…The core of the OEC is the combination of Mn cluster in Ca 2+ and Cl -, so Clplays an important role in stabilizing OEC functions. In this study, the activity of OEC on the donor side of PSII did not decrease significantly under NaCl stress, which may be related to the role of Cl - (Pang et al, 2010;Zhang et al, 2016b). However, under NaHCO3 stress, except for the significant increase of PPD4 expression, the expressions of other related proteins in the PSII donor side were significantly decreased compared with CK.…”

Section: ．Discussion 31 Response Of Photosynthesis and Related Protcontrasting

confidence: 51%

Photosynthetic Function and the Photoprotective Mechanism of Leaves of <em>Morus alba L.</em> Seedlings under NaCl and NaHCO3 Stress Revealed by Proteomics

Zhang¹,

Xu²,

X³

et al. 2019

Preprint

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Photosynthetic function, photoprotection, and the response of related proteomics of mulberry (Morus alba L.) seedling leaves under NaCl and NaHCO3 stress with the same Na+ concentration (100 mmol•L-1) were studied by using photosynthetic gas exchange and chlorophyll fluorescence techniques combined with TMT proteomics. The results showed that NaCl stress had no significant effect on photosystem II (PSII) activity in mulberry seedling leaves, and the expressions of the related proteins, OEE3-1 and PPD4, of the PSII oxygen-evolving complex (OEC) and the antenna proteins, CP24 10A, CP26, and CP29, of LHCII in the leaves also increased to varying degrees. The photosystem I (PSI) activity in the leaves of mulberry seedling also increased, and the expressions of some proteins, PsaF, PsaG, PsaH, PsaL, PsaN, and Ycf4, in PSI increased significantly under NaCl stress. Under NaHCO3 stress, the activity of PSII and PSI and the expression of their protein complexes and the electron transfer-related proteins significantly decreased. NaCl stress had little effect on RuBP regeneration during dark reaction in the leaves and the expressions of glucose synthesis related proteins and net photosynthetic rate (Pn) did not decrease significantly. The leaves could adapt to NaCl stress by reducing stomatal conductance (Gs) to increase water use efficiency (WUE). Under NaHCO3 stress, the expression of dark reaction-related proteins was mostly down-regulated, and Gs was significantly reduced, which indicated that non-stomatal factors were important reasons for the significant inhibition of carbon assimilation. In the photoprotective mechanism under NaCl stress, the expression of cyclic electron flow (CEF) related proteins, ndhH, ndhI, ndhK, and ndhM, involved in NAD(P)H dehydrogenase (NDH) and the key enzyme of the xanthophyll cycle, violaxanthin de-epoxidase (VDE) were up-regulated. In addition, the ratio of xanthophyll cycle components (A+Z)/(V+A+Z) was increased. The expressions of proteins FTR and Fd-NiR, which are related to Fd-dependent ROS metabolism and nitrogen metabolism, were also significant up-regulated under NaCl stress, which can effectively reduce the electronic pressure on Fd. Under NaHCO3 stress, the expressions of CEF-related proteins, VDE, ZE, FTR, Fd-NiR, Fd-GOGAT, SGAT, and GGAT, were significant down-regulated, and the photoprotective mechanism, like the xanthophyll cycle, CEF, and photorespiration, might be damaged, resulting in the inhibition of PSII activity and carbon assimilation in leaves of mulberry seedling under NaHCO3 stress.

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“…The increase in relative variable fluorescence ( V K ) value at point K (0.3 ms) is considered as the specific marker for inhibition of activity at PSII electron donor side, especially the activity of oxygen evolution complex (OEC) (Jiang et al, 2008; Zhang et al, 2012). The increase in relative variable fluorescence ( V J ) at point J (2 ms) indicates that the electron transfer from Q A to Q B is blocked and there is accumulation of reduced Q A (Haldimann and Strasser, 1999, Zhang A.Q. et al, 2016; Zhang H.H.…”

Section: Discussionmentioning

confidence: 99%

Rootstock Alleviates Salt Stress in Grafted Mulberry Seedlings: Physiological and PSII Function Responses

Zhang

ShuBo

et al. 2018

Front. Plant Sci.

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This study investigated the effect of NaCl stress on Na+ and K+ absorption and transport by roots, nitrogen and phosphorus content in leaves, PSII photochemical activity and reactive oxygen species (ROS) in leaves of mulberry own-root seedlings and grafted seedlings. To determine the response, own-root seedlings of a high yielding mulberry cultivar, Tieba mulberry (Morus alba L.), and the grafted seedlings, obtained by using Qinglong mulberry with high salt tolerance as rootstock and Tieba mulberry as scion, were used. The Na+ content in roots and leaves of grafted seedlings was significantly lower than that in own-root seedlings under salt stress; while K+ content in roots and leaves of grafted seedlings was significantly higher than that in own-root seedlings. The root activity in grafted seedlings was significantly higher than that in own-root seedlings, as well as the content of nitrogen, phosphorous and water. PSII photochemical activity in leaves of grafted seedlings was less significantly affected by salt stress compared to own-root seedlings. The electron transport at the acceptor side of PSII from QA to QB was less affected by salt stress, which resulted in a significantly lower ROS content in leaves of grafted seedlings than that of own-root seedlings. Therefore, grafting high-yielding and good-quality Tieba mulberry with salt tolerant Qinglong mulberry as rootstock showed a relatively high salt tolerance. This may be because (1) the root system of rootstock presented high Na+ resistance and has selective absorption capacity for Na+ and K+ (2) the root system of rootstock prevented excess Na+ from being transported to aerial parts in order to reduce adverse effects of Na+ (3) the root system of rootstock had enhanced root activity under salt stress, which accelerated water and nutrient absorption (4) the leaves of grafted seedlings had higher PSII photochemical activity and electron transport rate compared with those of own-root seedlings under salt stress, which effectively reduced ROS burst mediated by photosynthesis and reduced oxidative damage.

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Global proteomic mapping of alkali stress regulated molecular networks in Helianthus tuberosus L.

Cited by 27 publications

References 75 publications

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Photosynthetic Function and the Photoprotective Mechanism of Leaves of <em>Morus alba L.</em> Seedlings under NaCl and NaHCO3 Stress Revealed by Proteomics

Rootstock Alleviates Salt Stress in Grafted Mulberry Seedlings: Physiological and PSII Function Responses

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