Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury

Lin, Yihan; Platt, Maryann P.; Fu, Haiyan; Gui, Yuan; Wang, Yanlin; Zhou, Dong; Yu, Yonghao

doi:10.1074/mcp.ra120.002235

Cited by 19 publications

(39 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the global proteome and phosphor-proteome of sepsis kidney injury also reveal that caspase-1 is significantly up-regulated in the cecal ligation and puncture-induced sepsis, which mediates gasdermin D cleavage and activation (33). Gasdermin D dependent pyroptosis cell death has been revealed for the first time in nonseptic acute kidney injury (34).…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus nt5 gene contributes to bacterial infection ability to form kidney abscess

Yang¹,

Liu²,

Xia

et al. 2022

Preprint

View full text Add to dashboard Cite

Staphylococcus aureus (S. aureus) is a commonly conditional infection pathogen, in which several key virulence genes are responsible for bacterial infection ability. The S. aureus nt5 gene, encoding 5’-nucleotidase, mediates bacterial nucleic acid pathway, yet it is nearly unknown of nt5 function for staphylococcal infection ability. Herein we have constructed S. aureus mutant with the gene nt5C166T silence (S. aureus Δnt5) by a CRISPR RNA-guided base editing system to investigate bacterial infection ability in vitro and in vivo. As expected, several nt5-related genes are disturbed in S. aureus Δnt5, in which gene transcription level of py is decreased compared with the wild-type S. aureus. Bacterial gene nt5 is downregulated and py/adk are upregulated when S. aureus is exposed to antibiotics daptomycin, which indicates nt5-mediated nucleic acid pathway is interfered upon with daptomycin treatment. Furthermore, the mutant Δnt5 displays about a 40-fold reduction of bacterial loading in mouse kidney on a mouse sepsis model, and the infection ability of Δnt5 is reduced than the wild-type bacteria. The gene nt5 contributes to S. aureus-infected abscess formation in mouse kidney, and the silence of nt5 gene promotes phagocytosis of S. aureus by mouse and human immunocytes. In general, our findings reveal nt5 silence impedes bacterial loading in kidney to form abscess but enhances S. aureus to be phagocytosed by host cell immune system in vitro and in vivo, which indicates that nt5 gene plays an important role in bacterial infection and immune evasion.

show abstract

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus nt5 gene contributes to bacterial infection ability to form kidney abscess

Yang¹,

Liu²,

Xia

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Sepsis-induced acute kidney injury (S-AKI) is often the cause of CKD progression to kidney failure. Lin et al [ 52 ] studied the chronological progression of S-AKI to CKD at a span of seven days in animal models at the kidney proteome and phosphoproteome levels. In addition, given earlier evidence supporting cell apoptosis and inflammatory cell infiltration as major cellular processes in the development and progression of AKI [ 53 ], Bcl2-associated agonist of cell death protein (Bad) and FAS-associated death domain protein (Fadd) were specifically analyzed.…”

Section: Proteomics In the Investigation Of Gut-kidney-axismentioning

confidence: 99%

Microbiome in Chronic Kidney Disease (CKD): An Omics Perspective

Lohia

Vlahou

Zoidakis

2022

Toxins

View full text Add to dashboard Cite

Chronic kidney disease (CKD) is predominant in 10% of the world’s adult population, and is increasingly considered a silent epidemic. Gut microbiota plays an essential role in maintaining host energy homeostasis and gut epithelial integrity. Alterations in gut microbiota composition, functions and, specifically, production of metabolites causing uremic toxicity are often associated with CKD onset and progression. Here, we present the latest omics (transcriptomics, proteomics and metabolomics) studies that explore the connection between CKD and gut microbiome. A review of the available literature using PubMed was performed using the keywords “microb*”, “kidney”, “proteom”, “metabolom” and “transcript” for the last 10 years, yielding a total of 155 publications. Following selection of the relevant studies (focusing on microbiome in CKD), a predominance of metabolomics (n = 12) over transcriptomics (n = 1) and proteomics (n = 6) analyses was observed. A consensus arises supporting the idea that the uremic toxins produced in the gut cause oxidative stress, inflammation and fibrosis in the kidney leading to CKD. Collectively, findings include an observed enrichment of Eggerthella lenta, Enterobacteriaceae and Clostridium spp., and a depletion in Bacteroides eggerthii, Roseburia faecis and Prevotella spp. occurring in CKD models. Bacterial species involved in butyrate production, indole synthesis and mucin degradation were also related to CKD. Consequently, strong links between CKD and gut microbial dysbiosis suggest potential therapeutic strategies to prevent CKD progression and portray the gut as a promising therapeutic target.

show abstract

“…SerpinA3 is also expressed by carcinomas regulating apoptosis and invasiveness [ 59 , 60 ]. SeprinA-3N was demonstrated by others to be upregulated in the kidney 60-fold 2 days after sepsis-induced renal hypoxia [ 61 ] in a proteomic study. Serpina3N produced in tubular epithelial cells was elevated in the urine of rats with early AKI-to-CKD transition [ 62 ].…”

Section: Known Roles Of Major Acute-phase Proteins Detected By Ngs In...mentioning

confidence: 99%

A New Role of Acute Phase Proteins: Local Production Is an Ancient, General Stress-Response System of Mammalian Cells

Hamar

2022

IJMS

View full text Add to dashboard Cite

The prevailing general view of acute-phase proteins (APPs) is that they are produced by the liver in response to the stress of the body as part of a systemic acute-phase response. We demonstrated a coordinated, local production of these proteins upon cell stress by the stressed cells. The local, stress-induced APP production has been demonstrated in different tissues (kidney, breast cancer) and with different stressors (hypoxia, fibrosis and electromagnetic heat). Thus, this local acute-phase response (APR) seems to be a universal mechanism. APP production is an ancient defense mechanism observed in nematodes and fruit flies as well. Local APP production at the tissue level is also supported by sporadic literature data for single proteins; however, the complex, coordinated, local appearance of this stress response has been first demonstrated only recently. Although a number of literature data are available for the local production of single acute-phase proteins, their interpretation as a local, coordinated stress response is new. A better understanding of the role of APPs in cellular stress response may also be of diagnostic/prognostic and therapeutic significance.

show abstract

Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury

Cited by 19 publications

References 107 publications

Staphylococcus aureus nt5 gene contributes to bacterial infection ability to form kidney abscess

Staphylococcus aureus nt5 gene contributes to bacterial infection ability to form kidney abscess

Microbiome in Chronic Kidney Disease (CKD): An Omics Perspective

A New Role of Acute Phase Proteins: Local Production Is an Ancient, General Stress-Response System of Mammalian Cells

Contact Info

Product

Resources

About