Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures

Virulence of serotype M3 Group AStreptococcusstrains in wax worms (Galleria mellonellalarvae)

“…27 Growth at either 29°C or 40°C significantly altered the expression level of multiple proven and putative virulence factors, including genes implicated in carbohydrate and fatty acid metabolism, iron homeostasis and oxidative stress response, and cell wall and envelope biosynthesis. 27 Similar temperature-dependent transcriptome changes have also been reported for Streptococcus agalactiae and Streptococcus pneumoniae, 44,45 emphasizing the importance of this trait. In contrast, alternative invertebrate model hosts such as Drosophila melanogaster and Caenorhabditis elegans cannot be maintained at 37°C.…”

Evaluation of microbial RNA extractions from Streptococcus pneumoniae

“…For example, one study reported that transcription of approximately 38% (658 genes) of the SP genome (1717 genes) was modified ( 1.5-fold) by varying temperature over a range from 21 to 40 o C (Pandya et al, 2005). Moreover, this gene expression cycling is extremely rapid with the phase delay between environmental signal and transcript expression measured in minutes and the subsequent silencing of gene expression being under constitutive control by processes that rapidly degrade "free" mRNA (Bernstein et al, 2002;Bernstein et al, 2004;Hambraeus et al, 2003;Patel et al, 1991).…”

Global transcription profiling and virulence potential of Streptococcus pneumoniae after serial passage

“…RNA quality was assessed spectrophotometrically and by gel electrophoresis. The florescence-labeled cDNA was generated through random priming and reverse transcription as described previously (Pandya et al, 2005).…”

“…To survive and progress to diverse niches in host, the microorganism has developed mechanisms to adapt to these diverse sites and the unique microenvironments that may exhibit differences in the availability of nutrients, changes in pH and osmolarity or growth temperature (Aguilar et al, 1997;Konkel and Tilly, 2000;Borah et al, 2002;Pucci et al, 2004;Pandya et al, 2005;Matsunaga et al, 2007). The mechanism(s) that S. pneumoniae uses to adapt to these different environments remains unknown.…”

“…The development of DNA microarray technology and the complete annotation of the S. pneumoniae genome (Dopazo et al, 2001;Hoskins et al, 2001;Tettelin et al, 2001;Lanie et al, 2007), have provided optimal tools to begin to understand the mechanism of pneumoccocal disease at the gene expression (Mascher et al, 2003;McDaniel et al, 2004;Martin-Galiano et al, 2005;Pandya et al, 2005;Marrer et al, 2006;Hendriksen et al, 2007;McKessar and Hakenbeck, 2007) and proteomic levels Lee et al, 2006;Kwon et al, 2007). Here we report the global transcriptional profiling of S. pneumoniae ATCC 6304 serotype 4 after extended serial passage of 50× (50P) and 100× (100P) on sheep blood agar (BA) plates.…”