Global prevalence of dengue and chikungunya coinfection: A systematic review and meta-analysis of 43,341 participants

Irekeola, Ahmad Adebayo; Syafirah, E.A.R. Engku Nur; Islam, Asiful; Shueb, Rafidah Hanim

doi:10.1016/j.actatropica.2022.106408

Cited by 13 publications

(14 citation statements)

References 112 publications

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“…Moreover, the co-circulation of both viruses and of the vectors facilitates the occurrence of concurrent infection in the human population. 24 An association between viral RNA in raw urban wastewater and clinical surveillance could not be established, given the absence of data, encompassing both autochthonous and travel-associated cases, of CHIKV and DENV in Portugal from August 2022 to July 2023 (Turismo de Portugal: https://travelbi.turismodeportugal.pt/turismo-em-portugal/turismo-em-numeros-2022/. Last accessed: October 2023).…”

Section: Discussionmentioning

confidence: 99%

Wastewater-based surveillance for tracing the circulation of Dengue and Chikungunya viruses

Monteiro,

Pimenta,

Nunes

et al. 2023

Preprint

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SummaryBackgroundArboviral diseases, transmitted by infected arthropods, pose significant economic and societal threats. Their global distribution and prevalence have increased in recent years, driven by factors such as climate change, biodiversity loss and urbanization. These diseases are often underestimated due to uneven surveillance and asymptomatic cases. Current surveillance relies on monitoring vector occurrence and spatial distribution, as well as syndromic monitoring. In this work, we aimed to explore the utility of wastewater-based surveillance as an additional, added-value tool for vector-borne viruses tracking.MethodsA retrospective wastewater-based surveillance survey was conducted at ten wastewater treatment plants covering a large part of Portugal mainland (North, Lisboa and Vale do Tejo and South regions). Using RT-qPCR, we quantified the RNA concentrations of Dengue (DENV) and Chikungunya (CHIKV) viruses for a period of 12 months (n= 273 raw wastewater samples), ranging May 2022 - April 2023.FindingsDENV was detected in 25% of the samples, with concentrations spanning from 3·5 to 6·8 log copies/L. CHIKV was detectable in 11% of the samples, with concentrations up to 6·3 log copies/L. Notably, the occurrence of DENV and CHIKV was rather similar between the three regions. The Lisboa and Vale do Tejo region exhibited in general the highest median concentration for DENV and CHIKV following normalization with crAssphage (1·5 × 10-4and 1·1 × 10-3, respectively).InterpretationWe demonstrate the efficacy of wastewater-based surveillance as a potent tool for gauging the epidemiological landscape of both DENV and CHIKV in mainland Portugal, where autochthonous cases have not been detected yet. Therefore, WBS should be integrated as a supplementary component to standard surveillance strategies.Research in contextEvidence before this studyWastewater serves as a valuable resource for wastewater-based surveillance (WBS). This surveillance method involves analyzing biomarkers of human metabolism, activities and lifestyle in wastewater to gain insights into public health trends. Previously, WBS has been employed to track chemical substances like illicit drugs, including cocaine, as well as oseltamivir during the 2009 influenza pandemic. It has also played a crucial role in the global polio eradication program by contributing to assess poliovirus circulation in populations and evaluating immunization effectiveness.During the SARS-CoV-2 pandemic, there was a significant boost in wastewater-based surveillance. To the best of our knowledge, our study represents the first extensive application of this approach to comprehensively investigate and identify the presence of vector-borne viruses RNA such as chikungunya and dengue.In December 2022, we conducted a search on PubMed and Google Scholar for publications using the search terms “wastewater” and “dengue virus” or “chikungunya virus” or “West Nile virus” or “Zika virus”, encompassing manuscripts in all languages. Our search yielded only one study that focused on detecting arboviruses in wastewater, specifically Dengue. Surprisingly, the authors were unable to detect viral RNA despite the reported cases of Dengue between 2017 and 2019, when the wastewater samples were collected. This period included a major outbreak in 2019, with more than 7,000 confirmed cases. Remarkably, no other study has shed light on the presence and concentration of Dengue and Chikungunya viral RNA in wastewater.Added value of this studyIn response to the gap identified in the literature review, this study makes a significant contribution by reporting for the first time the presence and concentration of arboviral RNA, specifically focusing on the Dengue and Chikungunya viruses. Notably, it has demonstrated the circulation of these viruses, even when only a few travel-associated cases have been reported throughout the years and where no autochthonous have been reported within the community, although the vector (Aedes albopictus) has been identified in several occasions in mainland Portugal. This research underscores the vital role of wastewater-based surveillance in gaining insights into the circulation trends of underreported pathogens within communities at the sewershed level.Implications of the available informationArboviral infections are significantly underreported, especially in non-endemic countries, and they can lead from mild to severe health issues and even mortality. The symptoms are unspecific and resemble those of various other illnesses, such as Influenza and COVID-19. Consequently, many infected individuals may not seek medical assistance, and when they do, their infections might be misdiagnosed. This results in surveillance for arboviral infections being biased toward individuals presenting with unusual symptoms or severe illness, or returning from endemic regions, leading to delayed reporting and limited temporal and spatial accuracy.In contrast, wastewater surveillance provides results within 24 hours of sample collection and offers a representation of the population served by the sewer system. This includes asymptomatic individuals and those with mild symptoms who may not seek medical care. This approach increases the likelihood of detecting viral circulation and thus prompt public health intervention, namely through vector control, limiting the potential transmission to new hosts, breaking the cycle of transmission.Wastewater surveillance data can play a crucial role in informing public health and environmental decision-making. It can reveal the presence of unaccounted-for infections, indicating the need for control and mitigation strategies, both at the population and mosquito vector levels. Although the arboviral activity is assumed residual in Portugal, wastewater monitoring can be used in complement to the efforts that are already done under the National Vector Surveillance Network. Moreover, this data can be used to communicate with the general public, encouraging them to take actions that can help control the mosquito population, such as reducing stagnant water sources where mosquitoes lay their eggs. This is especially important for vulnerable populations, enabling them to make more informed decisions to mitigate the underlying risks effectively.

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Section: Discussionmentioning

confidence: 99%

Wastewater-based surveillance for tracing the circulation of Dengue and Chikungunya viruses

Monteiro,

Pimenta,

Nunes

et al. 2023

Preprint

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“…Serology may be required to differentiate both of these infections. A meta‐analysis of data from 43,341 participants revealed a global prevalence of 2.5% of dengue‐chikungunya coinfection 47 . In a cross‐sectional study from Punjab, Kaur et al 46 .…”

Section: Differential Diagnosesmentioning

confidence: 99%

“…A meta-analysis of data from 43,341 participants revealed a global prevalence of 2.5% of dengue-chikungunya coinfection. 47 In a cross-sectional study from Punjab, Kaur et al 46 reported a coinfection rate of (virus-like particle). 10 The prevention of chikungunya fever epidemic is mainly aimed at vector control measures.…”

Section: Chikungunya Mimics Dengue Viral Infection Very Closely Andmentioning

confidence: 99%

Mucocutaneous manifestations of chikungunya fever: an update

Vinay,

Thind,

Mehta

et al. 2023

Int J Dermatology

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Chikungunya is a viral disease transmitted by female Aedes mosquitoes that has been increasingly reported in many parts of the world across the geographical borders. In addition to fever and joint pain, mucocutaneous manifestations of chikungunya have been reported in 40–75% of infected patients. Dermatological manifestations of chikungunya are often under‐recognized and misdiagnosed as clinicians are not sensitized or educated regarding these. The early‐onset cutaneous manifestations of chikungunya fever, occurring within 1 month of the fever, include maculopapular rashes, vesiculobullous eruptions, Steven‐Johnson syndrome/toxic epidermal necrolysis‐like eruptions, flagellate lesions, scrotal dermatitis, oro‐genital ulcers, and exacerbation of preexisting dermatoses like psoriasis. Hyperpigmentation, lichenoid eruptions, diffuse hair fall, and exacerbation of acne usually occur as a late manifestation. Diagnosis of these mucocutaneous manifestations can be challenging as they often resemble other common dermatoses. This review article elaborates on various mucocutaneous manifestations of chikungunya fever, based on literature review and authors’ clinical experience.

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“…The prevalence and spread of mosquito-borne diseases are rapidly increasing globally. [1][2][3] Aedes, Anopheles, and Culex mosquitoes are the primary vectors of diseases like malaria, dengue fever, zika, chikungunya, West Nile fever, yellow fever, and tularemia. [1][2][3][4][5][6] Climate change, socioeconomic factors, and inter-border travelling have all contributed to the expansion and proliferation of these diseases and their vectors, posing an existential threat to the health and well-being of over four billion people across over hundred countries.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Aedes, Anopheles, and Culex mosquitoes are the primary vectors of diseases like malaria, dengue fever, zika, chikungunya, West Nile fever, yellow fever, and tularemia. [1][2][3][4][5][6] Climate change, socioeconomic factors, and inter-border travelling have all contributed to the expansion and proliferation of these diseases and their vectors, posing an existential threat to the health and well-being of over four billion people across over hundred countries. 7 Despite the urgent need to curb the associated morbidity of mosquito-borne diseases and the proliferation of mosquitoes, mosquitoes have proved remarkably resilient, adapting to a wide range of habitats and developing resistance to existing insecticides.…”

Section: Introductionmentioning

confidence: 99%

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

2024

TJNPR

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An antibiotic-producing Streptomyces spp. was isolated from forest soil and assessed for its biolarvicidal and cytotoxic activity against various mosquito species (Aedes spp, Anopheles spp, Culex spp, and Mansonia spp) and Brime shrimps. Active biocides were extracted using a modified solid state fermentation process. Mosquito larvae were exposed to different concentrations of the ethanolic extract of the Streptomyces exudates for 24 hours. The extract's cytotoxicity was also evaluated using Brime shrimp. The results revealed a significant mortality rate among all four important vector mosquitoes following treatment with the extract. The LC50 and LC90 values of the extract were 0.40 mg/ml and 0.80 mg/ml for Anopheles spp, 0.20 mg/ml and 0.60 mg/ml for Culex spp, 0.40 mg/ml and 0.60 mg/ml for Aedes spp, and 0.20 mg/ml and 0.60 mg/ml for Mansonia spp, respectively. The Minimum larvicidal concentration values were 0.2 mg/ml for Aedes spp, Anopheles spp, Culex spp, and Mansonia spp. The extract showed a low degree of cytotoxicity, with an average mortality rate of only 20% at 0.8 mg/ml of the extract, upon testing with Brime shrimp. In conclusion, the aqueous extract of forest Streptomyces spp. exhibited high bio-larvicidal activity against Aedes spp, Anopheles spp, Culex spp, and Mansonia spp larvae, suggesting its potential as an environmentally friendly approach to mosquito control. This study represents an initial step toward supplementing eco-friendly nontoxic microbe-based bioinsecticides for synthetic insecticides against medically significant mosquitoes.

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Global prevalence of dengue and chikungunya coinfection: A systematic review and meta-analysis of 43,341 participants

Cited by 13 publications

References 112 publications

Wastewater-based surveillance for tracing the circulation of Dengue and Chikungunya viruses

Wastewater-based surveillance for tracing the circulation of Dengue and Chikungunya viruses

Mucocutaneous manifestations of chikungunya fever: an update

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

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