Global omics strategies to investigate the effect of cyclodextrin nanoparticles on MCF-7 breast cancer cells

Insight into oral amphiphilic cyclodextrin nanoparticles for colorectal cancer: comprehensive mathematical model of drug release kinetic studies and antitumoral efficacy in 3D spheroid colon tumors

“…Similar results were obtained in cell culture studies performed on different cancer cells by our group, and detailed studies were carried out to elucidate the mechanism. Both the results of our studies and the literature emphasize that cyclodextrins show high affinity for lipid-based molecules such as cholesterol and phospholipids in biological membranes [50,55,56]. Furthermore, it was reported that depletion of cholesterol by methyl-β-cyclodextrin could inhibit EGFR signals, induce apoptosis, and suppress tumor growth in colon tumor-induced mice [57].…”

Polycationic cyclodextrin nanoparticles induce apoptosis and affect antitumoral activity in HepG2 cell line: An evaluation at the molecular level

“…Authors thank reviewer for his/her critical evaluation. The shotgun proteomics approach that we used in our study is not like untargeted metabolomics approach that we used in our previous studies [15]. Therefore, volcano plots are not useful to present proteomics data since the statistical comparison is performed not only with LC-MS peak areas of the peptides but with the LC-MS/MS data.…”

Therapeutic Efficacy and Biodistribution of Paclitaxel-Bound Amphiphilic Cyclodextrin Nanoparticles: Analyses in 3D Tumor Culture and Tumor-Bearing Animals In Vivo

“…In our previous studies, it was observed that blank amphiphilic CD derivatives cause a selective antiproliferative effect in various cancer cells (MCF-7, HepG2, MDA-MB-231), although they do not have any toxic effects on healthy fibroblasts (L929) and healthy bladder cells (G/G1) [33]. With advanced cell culture studies performed with proteomic and metabolomic approaches, it was determined that this effect was caused by the cholesterol affinity of CDs [32]. It is known that amount of cholesterol in the membranes of cancer cells is higher than that in healthy cells.…”

“…In addition, the findings obtained by RT-PCR showed that the 6OCaproβCD nanoparticle, which can be used as a nanoparticular drug carrier, does not trigger multidrug resistance. The effect of the metabolomic pathways affected by blank 6OCaproβCD nanoparticles on breast cancer cells was elucidated with Human Metabolome Database (HMDB) as serine biosynthesis, transmembrane transport of small molecules, metabolism of steroid hormones, estrogen biosynthesis, and phospholipid biosynthesis [32].…”

“…Our formulation development studies revealed both CDs as optimal carriers for PCX in our previous studies through cell culture studies and detailed in vitro characterization data including particle size, drug-loading efficiency, drug release profile, stability, hemolysis, and cytotoxicity in healthy cells [15,31]. Moreover, in our previous papers, it was determined that these two amphiphilic CD derivatives even in blank form had a high affinity to cholesterol microdomains in the cell membrane in human breast cancer cells (MCF-7), as well as removing cholesterol from the membrane and inducing apoptosis in cells in a dose-dependent manner that can be beneficial in fighting multidrug resistance based on cell membrane rigidity [32,33]. In this study, the antitumoral activities of anionic and cationic amphiphilic CD nanoparticles dispersions, which are ideal carrier systems for PCX, were evaluated for tumoral penetration in 3D spheroid tumor culture and antitumoral and antimetastatic efficacy in tumor-induced mice studies.…”