Global occurrence of deoxynivalenol in food commodities and exposure risk assessment in humans in the last decade: a survey

Mishra, Sakshi; Srivastava, Sonal; Dewangan, Jayant; Divakar, Aman; Rath, Srikanta Kumar

doi:10.1080/10408398.2019.1571479

Cited by 156 publications

(106 citation statements)

References 202 publications

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“…It has been reported that DON can cause animals to food refusal, organ damage, and increase the risk of disease [6][7][8]. It is generally believed that DON exerts many toxic effects such as neurotoxicity, immuno-toxicity, intestinal toxicity, and cytotoxicity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway

Wang

Zhang

Zhao

et al. 2019

Toxins

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The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of IL-1β and IL-6 were increased in the cells. The mRNA relative expression of NF-κB p65, IKKα, and IKKβ were upregulated with the increasing of DON concentration, while the relative expression of IκB-α mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.

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Section: Introductionmentioning

confidence: 99%

Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway

Wang

Zhang

Zhao

et al. 2019

Toxins

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“…To lower the amount or avoid CER formation in foods, proper storage, especially cooling, is essential (Delbrassinne et al 2012). Furthermore, DON is a known global food contaminant (Mishra et al 2019) and the co-occurrence of DON and CER was recently observed in Cameroonian maize fufu (Abia et al 2017). For evaluation of the risk assessment, further investigations are needed.…”

Section: Discussionmentioning

confidence: 99%

“…To mimic inflammatory conditions, cells were stimulated with 25 ng/mL IL-1β as previously described for Caco-2 monolayers (Van De Walle et al 2008, 2010b). Exposure levels are based on occurrence data (Abia et al 2017;Mishra et al 2019;Schothorst and van Egmond 2004) and are described in detail in the discussion section. All experiments were performed with at least three biological replicates.…”

Section: Incubation Conditions and Justification Of Exposurementioning

confidence: 99%

“…Inhibition of protein biosynthesis is considered the main mechanism of action of DON (Pinton and Oswald 2014). Mishra et al (2019) recently reviewed the worldwide occurrence of DON in foodstuffs and concluded that DON was present in a wide concentration range in grains like wheat, maize or oats and cereal-based products as well as infant food. Consequently, humans are generally exposed to at least low doses of this toxin through their diet, with the gastrointestinal tract being first affected by foodborne toxins.…”

Section: Introductionmentioning

confidence: 99%

“…The applied toxin concentrations were chosen based on occurrence data (Abia et al 2017;Bauer et al 2018;Delbrassinne et al 2012;Mishra et al 2019;Schothorst and van Egmond 2004) and under the previously reported assumption that the substances are diluted in the intestine in 1 L gastric fluid and are fully bio-accessible (Ling et al 2016;Sergent et al 2006). Doses up to 10 µg/mL DON have previously been considered realistic (Van De Walle et al 2008).…”

mentioning

confidence: 99%

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Combinatory effects of cereulide and deoxynivalenol on in vitro cell viability and inflammation of human Caco-2 cells

et al. 2020

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Deoxynivalenol (DON), one of the most abundant mycotoxins in cereal products, was recently detected with other mycotoxins and the emetic bacterial toxin cereulide (CER) in maize porridge. Within a cereal-based diet, co-exposure to these toxins is likely, hence raising the question of combinatory toxicological effects. While the toxicological evaluation of DON has quite progressed, consequences of chronic, low-dose CER exposure are still insufficiently explored. Information about the combinatory toxicological effects of these toxins is lacking. In the present study, we investigated how CER (0.1-100 ng/mL) and DON (0.01-10 µg/mL) alone and in a constant ratio of 1:100 (CER:DON) affect the cytotoxicity and immune response of differentiated human intestinal Caco-2 cells. While DON alone reduced cell viability only in the highest concentration (10 µg/mL), CER caused severe cytotoxicity upon prolonged incubation (starting from 10 ng/mL after 24 h and 48 h, 2.5 ng/ mL and higher after 72 h). After 72 h, synergistic effects were observed at 2.5 ng/mL CER and 0.25 µg/mL DON. Different endpoints of inflammation were investigated in interleukin-1β-stimulated Caco-2 cells. Notably, DON-induced interleukin-8 transcription and secretion were diminished by the presence of 10 and 25 ng/mL CER after short-term (5 h) incubation, indicating immunosuppressive properties. We hypothesise that habitual consumption of cereal-based foods co-contaminated with CER and DON may cause synergistic cytotoxic effects and an altered immune response in the human intestine. Therefore, further research concerning effects of co-occurring bacterial toxins and mycotoxins on the impairment of intestinal barrier integrity, intestinal inflammation and the promotion of malnutrition is needed.

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Decontamination and Detoxification of Deoxynivalenol – An Emetic Toxin of Food and Feed

Sumalatha,

Maheshwar

2023

Anti‐Mycotoxin Strategies for Food and Feed

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Global occurrence of deoxynivalenol in food commodities and exposure risk assessment in humans in the last decade: a survey

Cited by 156 publications

References 202 publications

Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway

Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway

Combinatory effects of cereulide and deoxynivalenol on in vitro cell viability and inflammation of human Caco-2 cells

Decontamination and Detoxification of Deoxynivalenol – An Emetic Toxin of Food and Feed

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