2004
DOI: 10.1128/mcb.24.15.6773-6787.2004
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Global mRNA Stabilization Preferentially Linked to Translational Repression during the Endoplasmic Reticulum Stress Response

Abstract: The stability of mRNAs undergoing translation has long been a controversial question. Here, we systematically investigate links between mRNA turnover and translation during the endoplasmic reticulum (ER) stress response, a process during which protein synthesis is potently regulated. cDNA array-based approaches to assess the stability and translational status of each mRNA were devised. First, ER stress-triggered changes in mRNA stability were studied by comparing differences in steady-state mRNA levels with di… Show more

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Cited by 75 publications
(89 citation statements)
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References 51 publications
(50 reference statements)
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“…Our previous studies had uncovered global links between mRNA stabilization and translational control during ER stress (22). We obtained systematic evidence that altered mRNA turnover strongly influenced changes in steady-state mRNA levels during the ER stress response and that translation was widely repressed.…”
mentioning
confidence: 86%
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“…Our previous studies had uncovered global links between mRNA stabilization and translational control during ER stress (22). We obtained systematic evidence that altered mRNA turnover strongly influenced changes in steady-state mRNA levels during the ER stress response and that translation was widely repressed.…”
mentioning
confidence: 86%
“…For Western blot analysis, whole-cell (10 g), cytoplasmic (20 g), and nuclear (10 g) lysates were prepared (22), size fractionated by electrophoresis through Tris-HCl gels (Bio-Rad, Life Science), and transferred onto polyvinylidene difluoride membranes. Monoclonal antibodies recognizing HuR and ␣-tubulin (a control cytoplasmic protein) as well as goat polyclonal anti-TIA-1 and anti-TIAR antibodies and a rabbit polyclonal antibody recognizing hnRNP C1/C2 (a control nuclear protein) were from Santa Cruz Biotechnology.…”
Section: Methodsmentioning
confidence: 99%
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“…Recent findings indicate that ER stress might alter gene expression beyond protein folding (34). The ER stress inducers TG and TM are able to enhance the expression of differentiation genes in addition to genes encoding secretory polypeptides, membrane proteins, and growth factors (18 -20).…”
Section: Discussionmentioning
confidence: 99%
“…stress can active a number of signaling pathways (22,33,34). Previous studies reported that the TGF-␤/Smad, Wnt/␤-catenin, and FGF signaling pathways can regulate the formation of the definitive endoderm (2,4,7).…”
Section: Tgf-␤/smad Signaling In the Definitive Endodermal Specificatmentioning
confidence: 99%