Global Methylation Pattern of Genes in Androgen-Sensitive and Androgen-Independent Prostate Cancer Cells

Mishra, Dhruva K.; Chen, Zujian; Wu, Yanyuan; Sarkissyan, Marianna; Koeffler, H. Phillip; Vadgama, Jaydutt V.

doi:10.1158/1535-7163.mct-09-0486

Cited by 70 publications

(65 citation statements)

References 26 publications

(29 reference statements)

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“…2, B-D, showed that there are significant differences in methylation in CpG islands of AR promoter in the various types of stem/progenitor cells, which may explain the differential AR expression in the various types of stem/progenitor cells (41,42).…”

Section: Differential Ar Expression In Different Types Of Stem/progenmentioning

confidence: 98%

“…tion, and tested whether AR expression profiles in the three types of stem/progenitor cell are changed but found no significant differences (data not shown). We next investigated whether the AR expression difference is due to the methylation difference in the AR promoter-associated CpG island region because numerous studies demonstrated that the methylation of these CpG islands interferes with transcription, resulting in an absence of mRNA and protein expression (41,42).…”

Section: Differential Ar Expression In Different Types Of Stem/progenmentioning

confidence: 99%

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Targeting the Unique Methylation Pattern of Androgen Receptor (AR) Promoter in Prostate Stem/Progenitor Cells with 5-Aza-2′-deoxycytidine (5-AZA) Leads to Suppressed Prostate Tumorigenesis

Tian

Lee

Liang

et al. 2012

Journal of Biological Chemistry

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Background: There is a specific silencing of AR gene expression in prostate stem cells. Results:The induction of AR gene expression inhibited the self-renewal of the prostate stem cells. Conclusion:The induction of AR expression suppressed PCa stem cell-mediated tumorigenicity. Significance: This is the first attempt to suppress prostate cancer growth via epigenetic modification of AR genes in PCa stem cells.

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Section: Differential Ar Expression In Different Types Of Stem/progenmentioning

confidence: 98%

Section: Differential Ar Expression In Different Types Of Stem/progenmentioning

confidence: 99%

Targeting the Unique Methylation Pattern of Androgen Receptor (AR) Promoter in Prostate Stem/Progenitor Cells with 5-Aza-2′-deoxycytidine (5-AZA) Leads to Suppressed Prostate Tumorigenesis

Tian

Lee

Liang

et al. 2012

Journal of Biological Chemistry

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“…It should be noted however that the process of immortalization itself can result in genetic alterations and/or mutation (Stepanenko & Kavsan 2012). To date, these cell lines have predominantly been used to compare gene expression levels and drug efficacy with PCa cell lines (Kim et al 2007, Deep et al 2008, Mishra et al 2010.…”

Section: Benign Prostate Epithelial Cell Linesmentioning

confidence: 99%

In vitro model systems to study androgen receptor signaling in prostate cancer

Sampson

Neuwirt

Puhr

et al. 2013

Endocrine-Related Cancer

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Prostate cancer (PCa) is one of the most common causes of male cancer-related death in Western nations. The cellular response to androgens is mediated via the androgen receptor (AR), a ligand-inducible transcription factor whose dysregulation plays a key role during PCa development and progression following androgen deprivation therapy, the current mainstay systemic treatment for advanced PCa. Thus, a better understanding of AR signaling and new strategies to abrogate AR activity are essential for improved therapeutic intervention. Consequently, a large number of experimental cell culture models have been established to facilitate in vitro investigations into the role of AR signaling in PCa development and progression. These different model systems mimic distinct stages of this heterogeneous disease and exhibit differences with respect to AR expression/status and androgen responsiveness. Technological advances have facilitated the development of in vitro systems that more closely reflect the physiological setting, for example via the use of three-dimensional coculture to study the interaction of prostate epithelial cells with the stroma, endothelium, immune system and tissue matrix environment. This review provides an overview of the most commonly used in vitro cell models currently available to study AR signaling with particular focus on their use in addressing key questions relating to the development and progression of PCa. It is hoped that the continued development of in vitro models will provide more biologically relevant platforms for mechanistic studies, drug discovery and design ensuring a more rapid transfer of knowledge from the laboratory to the clinic.

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“…Both the long reaction time and the labor of this modification make this process hard. Thus, we used kit for modification and there are many studies which use these modification kits for their studies (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

Promoter hypermethylation of p16 and APC in gastrointestinal cancer patients

Erdem¹,

Küçükyıldırım²,

Saglar³

et al. 2014

Turk J Gastroenterol

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Background/Aims: Cancer is a consequence of the disruption of cellular regulation. Epigenetic is one of the reasons of this disruption. Epigenetic factors play a role in the carcinogenesis by affecting proto-oncogenes and tumor suppressor genes and it is one of the most popular research areas in recent years. DNA methylation, which is an epigenetic mechanism, occurs in the early stages of tumorigenesis. Promoter methylation which causes the silence of tumor suppressor genes have been studied extensively in various tumor types. The aim of this study was to investigate promoter methylation of certain tumor suppressor genes, Cyclin-dependent kinase inhibitor 2A (p16) and Adenomatous polyposis coli (APC), which take part in gastrointestinal tumorigenesis. Materials and Methods: To detect the promoter methylation of p16 and APC genes, tissue samples from 20 gastrointestinal cancer patients and peripheral blood samples from 15 healthy individuals were collected for Methylation-Specific Polymerase Chain Reaction (MSP) analysis. Results: According to the statistical analysis, in tumor tissue, positive methylation ratio of p16 and APC genes was found respectively 30% (6/20) and 50% (10/20). The difference of promoter methylation of these genes between tumor tissues and control group was significantly observed (p=0.02 and 0.001, respectively). An alteration of promoter methylation of APC gene according to tumor localization was found (p=0.007), but there was no significant difference observed in p16. Conclusion: In our study, promoter methylation which was considered to be occurred as an early event in gastrointestinal carcinogenesis was observed in p16 and APC genes.

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Global Methylation Pattern of Genes in Androgen-Sensitive and Androgen-Independent Prostate Cancer Cells

Cited by 70 publications

References 26 publications

Targeting the Unique Methylation Pattern of Androgen Receptor (AR) Promoter in Prostate Stem/Progenitor Cells with 5-Aza-2′-deoxycytidine (5-AZA) Leads to Suppressed Prostate Tumorigenesis

Targeting the Unique Methylation Pattern of Androgen Receptor (AR) Promoter in Prostate Stem/Progenitor Cells with 5-Aza-2′-deoxycytidine (5-AZA) Leads to Suppressed Prostate Tumorigenesis

In vitro model systems to study androgen receptor signaling in prostate cancer

Promoter hypermethylation of p16 and APC in gastrointestinal cancer patients

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