Global identification and comparative analysis of SOCS genes in fish: Insights into the molecular evolution of SOCS family

Jin, Hong; Shao, Jian; Xiang, Li Xin; Wang, Hao; Sun, Li

doi:10.1016/j.molimm.2007.09.015

Cited by 73 publications

(85 citation statements)

References 35 publications

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“…CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Jin et al [19] reported that the expression levels of most SOCS genes, such as CISH, SOCS1-5 and SOCS9, were increased after LPS challenge, indicating that they are involved in inflammatory response. In the present study, Cish expression was highly increased (8.19-fold) by LPS treatment, but decreased in response to melatonin treatment.…”

Section: Discussionmentioning

confidence: 99%

Microarray Analysis of Gene Expression Profiles in Response to Treatment with Melatonin in Lipopolysaccharide Activated RAW 264.7 Cells

Ban

Kim

et al. 2011

Korean J Physiol Pharmacol

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Section: Discussionmentioning

confidence: 99%

Microarray Analysis of Gene Expression Profiles in Response to Treatment with Melatonin in Lipopolysaccharide Activated RAW 264.7 Cells

Ban

Kim

et al. 2011

Korean J Physiol Pharmacol

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“…Total RNA was isolated from kidney, spleen, and brain tissues by using an RNAiso Plus kit (Takara Bio), and DrTIM-1, DrTIM-4, and soluble (s)DrTIM-4 cDNAs were generated through RT-PCR by using primers (Supplemental Table I) and 39 full RACE core sets (Takara Bio) in accordance with the manufacturer's instructions (28). The PCR products were purified from agarose gel (1.2%) by using a gel extraction kit (Qiagen), inserted into the pGEM-T EASY vector (Promega), and then transformed into competent Escherichia coli TOP 10 (Invitrogen).…”

Section: Cloning Of Drtim-1 and Drtim-4 Cdnasmentioning

confidence: 99%

“…Gene organizations (intron/exon boundaries) were elucidated by comparing DrTIM-1 and DrTIM-4 cDNAs with genome sequences, and the figures were drawn using GeneMapper 2.5 (28). Full-length cDNAs of DrTIM-1 and DrTIM-4 were assembled using the CAP3 sequence assembly program (30).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Essential Roles of TIM-1 and TIM-4 Homologs in Adaptive Humoral Immunity in a Zebrafish Model

et al. 2016

The Journal of Immunology

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“…The mammalian CD154 and CD40 sequences were used as queries. After sequences similar to CD154 and CD40 were obtained by BLAST, they were further characterized using DNA and protein sequence analysis software with methods previously described (28). Genomic structures and organizations of fish CD154 and CD40 genes were predicted from genomic sequences using cDNAs or ESTs available from each species.…”

Section: Sequence Retrievalmentioning

confidence: 99%

CD154-CD40 Interactions Are Essential for Thymus-Dependent Antibody Production in Zebrafish: Insights into the Origin of Costimulatory Pathway in Helper T Cell-Regulated Adaptive Immunity in Early Vertebrates

Gong

Xiang²,

Shao³

2009

The Journal of Immunology

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The CD154-CD40-mediated costimulatory pathway is critical for T-B cell cooperation in thymus-dependent (TD) immune response in mammals. However, little is known about its existence and occurrence in lower vertebrates. Here, we report on the identification and functional characterization of CD154 and CD40 homologs from the zebrafish (Danio rerio) model. Zebrafish CD154 is a type II membrane-bound protein with a TNF homology domain in its extracellular C-terminal region, whose tertiary structure is a sandwich containing two stacked sheets with “jelly roll” topology, just as the human TNF members do. The zebrafish CD40 is a type I membrane-bound protein with a sequence pattern of four cysteine-rich domains in its extracellular N-terminal region. The consensus TNFR-associated factor (TRAF)2- and TRAF6-binding motifs in mammalian CD40 are found in the cytoplasmic tail of zebrafish CD40, which indicates similar signal transduction mechanisms to higher vertebrates. Zebrafish CD154 and CD40 are widely distributed and can be up-regulated by thymus-dependent Ag. The production of IgM was dramatically decreased by anti-CD154 or soluble CD40, and it was enhanced by soluble CD154 or CD154-encoding plasmid in vivo. Thymus-dependent Ag-induced CD154 expression was inhibited by cyclosporin A, suggesting that CD154 functionally associates with T cells. Double immunofluorescence staining showed that CD40 and membrane IgM colocalized in B cells. CD154-CD40 binding assays showed that CD154 specifically binds to CD40 at homodimeric form. Our results provide the first evidence for the existence of the functional CD154-CD40-mediated costimulatory pathway and helper T cell regulatory mechanism underlying adaptive immunity in a fish species.

show abstract

Global identification and comparative analysis of SOCS genes in fish: Insights into the molecular evolution of SOCS family

Cited by 73 publications

References 35 publications

Microarray Analysis of Gene Expression Profiles in Response to Treatment with Melatonin in Lipopolysaccharide Activated RAW 264.7 Cells

Microarray Analysis of Gene Expression Profiles in Response to Treatment with Melatonin in Lipopolysaccharide Activated RAW 264.7 Cells

Essential Roles of TIM-1 and TIM-4 Homologs in Adaptive Humoral Immunity in a Zebrafish Model

CD154-CD40 Interactions Are Essential for Thymus-Dependent Antibody Production in Zebrafish: Insights into the Origin of Costimulatory Pathway in Helper T Cell-Regulated Adaptive Immunity in Early Vertebrates

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